Optimized cationic lipid‐based gene delivery reagents for use in developing vertebrate embryos

deCastro, Michael J.; Saijoh, Yukio; Schoenwolf, Gary C.

doi:10.1002/dvdy.20873

Cited by 29 publications

(26 citation statements)

References 24 publications

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“…In comparison to other liposomal reagents published for chicken embryo work (Dickinson et al, 2002;Bollérot et al, 2006;Decastro et al, 2006), DODAC/DOPE (18 mM) appears to result in a qualitatively higher level of transfection, especially in the normally difficult to transfect mesenchyme. The lipoplexes also appear to give equivalent levels of limb mesenchyme transfection compared to electroporation of stage-15 lateral plate mesoderm (Sato et al, 2007;Boehm et al, 2010;Gros et al, 2010;Casanova et al, 2011).…”

Section: Adenovirusmentioning

confidence: 66%

“…Our first aim was to find a cationic/ helper lipid combination that would work better in vivo than off-the-shelf commercial reagents or customized reagents published by others for use in the chicken embryos (Muramatsu et al, 1997;Dickinson et al, 2002;Bollérot et al, 2006;Decastro et al, 2006). To start with, we tested three cationic lipids, DOTAP (Bollérot et al, 2006), DOTMA, and DODAC.…”

Section: Resultsmentioning

confidence: 99%

“…DODAC was developed by our group (Hafez et al, 2000) and had been shown to be a good transfection reagent in vitro (Mok and Cullis, 1997;Hope et al, 1998) Each of these cationic lipids was combined with the most commonly used helper lipid, DOPE . Furthermore, DOPE has been used by others for in vivo transfections (Bollérot et al, 2006;Decastro et al, 2006) and it is a component of the original Lipofectamine TM from Invitrogen (Carlsbad, CA).…”

Section: Adenovirusmentioning

confidence: 99%

“…Although several groups have published technical papers showing that liposomes can work in vivo, they are still not commonly used. In avian embryos, the results are variable depending on the tissue and stage being transfected (Muramatsu et al, 1997;Dickinson et al, 2002;Bollérot et al, 2006;Decastro et al, 2006;Albazerchi et al, 2007). In addition, some of the carrier systems are complicated to prepare, which can pose a barrier to widespread adoption by developmental biologists (Dickinson et al, 2002;Decastro et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

et al. 2011

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Here we report that highly concentrated cationic lipid/helper lipid‐nucleic acid complexes (lipoplexes) can facilitate reproducible delivery of a variety of oligonucleotides and plasmids to chicken embryos or to mouse embryonic mesenchyme. Specifically, liposomes composed of N,N‐dioleyl‐N,N‐dimethylammonium chloride (DODAC)/1,2 dioleoyl glycero‐3‐phosphorylethanolamine (DOPE) prepared at 18‐mM concentrations produced high levels of transfection of exogenous genes in vivo and in vitro. Furthermore, we report sufficient uptake of plasmids expressing interference RNA to decrease expression of both exogenous and endogenous genes. The simplicity of preparation, implementation, and relatively low toxicity of this transfection reagent make it an attractive alternative for developmental studies in post‐gastrulation vertebrate embryos. Developmental Dynamics 240:2108–2119, 2011. © 2011 Wiley‐Liss, Inc.

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Section: Adenovirusmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Adenovirusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

et al. 2011

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“…Since the first published report by Felgner and coworkers [1] almost two decades ago on the advent of DOTMA as a carrier of nucleic acids in gene delivery, continuing work on this synthetic lipid [2] and other cationic amphiphiles [3][4][5][6][7] as non-viral vectors is ongoing. To produce efficient gene expression, the cationic lipid must possess suitable structural features that promote effective transfection.…”

Section: Introductionmentioning

confidence: 99%

Effect of spacer attachment sites and pH-sensitive headgroup expansion on cationic lipid-mediated gene delivery of three novel myristoyl derivatives

Spelios

Nedd

Matsunaga

et al. 2007

Biophysical Chemistry

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The transfection activity and physicochemical properties of the dimyristoyl derivatives from three novel series of double-chained tertiary cationic lipids were compared. Two of the derivatives were constructed as isomers with different linkages of the same bis-(2-dimethylaminoethane) polar headgroup and hydrophobic chains to the diaminopropanol backbone, while the third was designed with a hydrophilic region containing only a single ionizable amine group. Such systematic molecular changes offer a great opportunity to delineate factors critical for transfection activity, which in this work include the intramolecular distance between the hydrophobic chains and pH-expandability of the polar headgroup. The physical studies comprised a variety of techniques, including pK a determination, Langmuir monolayer studies, fluorescence anisotropy, gel electrophoresis mobility shift assay, ethidium bromide displacement assay, particle size distribution, and zeta potential. These studies are crucial in the development of lipid-based gene delivery systems with improved efficacy. Physicochemical characterization revealed that a symmetric bivalent pH-expandable polar headgroup in combination with greater intramolecular space between the hydrophobic chains provide for high transfection activity through efficient binding and compaction of pDNA, increased acyl chain fluidity, and high molecular elasticity.

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Lipid nanoparticle‐mediated RNA delivery for immune cell modulation

Kim,

Teerdhala,

Padilla

et al. 2024

Eur J Immunol

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Lipid nanoparticles (LNPs) have emerged as the preeminent nonviral drug delivery vehicles for nucleic acid therapeutics, as exemplified by their usage in the mRNA COVID‐19 vaccines. As a safe and highly modular delivery platform, LNPs are attractive for a wide range of applications. In addition to vaccines, LNPs are being utilized as platforms for other immunoengineering efforts, especially as cancer immunotherapies by modulating immune cells and their functionality via nucleic acid delivery. In this review, we focus on the methods and applications of LNP‐based immunotherapy in five cell types: T cells, NK cells, macrophages, stem cells, and dendritic cells. Each of these cell types has wide‐reaching applications in immunotherapy but comes with unique challenges and delivery barriers. By combining knowledge of immunology and nanotechnology, LNPs can be developed for improved immune cell targeting and transfection, ultimately working toward novel clinical therapeutics.

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Optimized cationic lipid‐based gene delivery reagents for use in developing vertebrate embryos

Cited by 29 publications

References 24 publications

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

Effect of spacer attachment sites and pH-sensitive headgroup expansion on cationic lipid-mediated gene delivery of three novel myristoyl derivatives

Lipid nanoparticle‐mediated RNA delivery for immune cell modulation

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