Optimized antibody immobilization on natural silica-based nanostructures for the selective detection of <i>E. coli</i>

Widyasari, Diaz Ayu; Kristiani, Anis; Randy, Ahmad; Manurung, Robeth Viktoria; Dewi, Rizna Triana; Andreani, Agustina Sus; Yuliarto, Brian; Jenie, S. N. Aisyiyah

doi:10.1039/d2ra03143d

Cited by 6 publications

(7 citation statements)

References 50 publications

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“…The FTIR spectra obtained after the IgG antibodies were immobilized showed two bands corresponding to amide II and amide I associated with the immobilized antibodies. The following bands attributed to EDC/NHS were also observed: one at 1712 cm –1 assigned to CO bonds and one at 1780 cm –1 assigned to the vibrational mode generated by the formation of a −COO-NHS ester bond, as observed in Figure b. , …”

Section: Resultsmentioning

confidence: 84%

“…The following bands attributed to EDC/NHS were also observed: one at 1712 cm −1 assigned to C�O bonds and one at 1780 cm −1 assigned to the vibrational mode generated by the formation of a −COO-NHS ester bond, as observed in Figure 5b. 64,65 FTIR Detection Spectral Analysis. An interaction between a specific bioreceptor and an analyte produces a measurable signal that we can detect, as contact and binding depend on adaptive stability; however, stability can be affected by several factors (pH, temperature, ionic strength, incubation time, and concentration).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Specific Detection of Uropathogenic Escherichia coli via Fourier Transform Infrared Spectroscopy Using an Optical Biosensor

Vazquez-Gutierrez,

Reyes-López,

Ochoa

et al. 2024

ACS Omega

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Section: Resultsmentioning

confidence: 84%

Section: ■ Results and Discussionmentioning

confidence: 99%

Specific Detection of Uropathogenic Escherichia coli via Fourier Transform Infrared Spectroscopy Using an Optical Biosensor

Vazquez-Gutierrez,

Reyes-López,

Ochoa

et al. 2024

ACS Omega

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“…25 Peaks at 1560, 1486, and 1632 cm À1 correspond to scissor vibration of NH 2 groups and symmetric and asymmetric deformation modes of -NH 3 + groups. 24,43 Additionally, a peak at 1021 cm À1 was attributed to the stretching vibrations of Si-O bonds. 44 These absorbance peaks confirmed the modification of APTES on the Al-A surface.…”

Section: Molecular Dynamics Simulationsmentioning

confidence: 99%

Experimental and molecular dynamics simulation study on antifouling performance of antimicrobial peptide‐modified aluminum alloy surfaces

Liu,

Lou,

Bai

et al. 2024

Surface & Interface Analysis

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Marine biofouling poses a major challenge to ship navigation and hinders the development of the shipping industry. Urgent action is required to tackle this problem through the implementation of innovative strategies. Antimicrobial peptides have garnered considerable attention due to their outstanding effectiveness, wide range of activity, and eco‐friendly characteristics. This study involved grafting the antibacterial peptide andricin 01 (AIGHCLGATL) onto the surface of an aluminum alloy, thereby creating a modified surface with antibacterial properties. In summary, amino groups were introduced onto the surface of aluminum alloys through the silanization process using (3‐aminopropyl) triethoxysilane (APTES), and then the peptides were covalently immobilized on the treated surface using glutaraldehyde as a cross‐linking agent. The successful modification of the peptide was confirmed by Fourier transform‐infrared spectroscopy (FT‐IR) and X‐ray photoelectron spectroscopy (XPS) analysis. The antimicrobial peptide‐modified aluminum alloy surfaces exhibited significant bactericidal activity, killing 75.3% of Bacillus sp. and 85.5% of Escherichia coli, while achieving antifouling efficiencies of 88.6% and 90.7% against Bacillus sp. and E. coli, respectively. Furthermore, molecular dynamics simulations showed that the inserted of the peptides into the phospholipid membrane caused a change in the local membrane curvature, which eventually led to membrane rupture. These results provide valuable information for the application of antimicrobial peptides in the field of antifouling and the elucidation of antifouling mechanisms.

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“…The functionalized working electrode was then rinsed with the DI. functional groups that may enhance selectivity, increase biocompatibility, or make it simpler to immobilize biomolecules [17], [18].…”

Section: Experiments Setupmentioning

confidence: 99%

The optimization of self-assembled monolayer of thiols on screen-printed gold electrode

Rahim,

Harun,

Abdullah

et al. 2023

IJAAS

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<span>The activated gold-modified electrode surface for self-assembled monolayer (SAM) is suitable for N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), 16-mercaptohexadecanoic acid (16-MDA), and N-hydroxysuccinimide (NHS). The various substrates could potentially be used for surface functionalization that binds to one another. Using modified screen-printed gold electrodes, 16-MDA, EDC, and NHS, we developed a self-assembling monolayer. Different 16-MDA concentrations were applied to the surface of a screen-printed electrode surface to enhance the sensitivity of the working electrode. The impact of different EDC, NHS, and 16-MDA concentrations (0.4 M, 0.8 M, 1 M, 1.2 M, 1.4 M, and 1.8 M) and incubation times between 5 and 30 minutes were examined and compared. The binding surface of the screen-printed gold electrode was characterized using the differential pulse voltammetry (DPV) technique. It has been demonstrated that the substrate concentrations at 5 minutes and 30 minutes of incubation time used to have the highest surface coverage and electron transfer rates. This concentration would be utilized in the subsequent experiment to evaluate the binding of various bacterial species. These findings suggest that the SAM in a modified screen-printed gold electrode may be functionalized to detect microorganisms.</span>

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Optimized antibody immobilization on natural silica-based nanostructures for the selective detection of E. coli

Abstract: This work describes for the first time the surface modification of natural-based fluorescent nanoparticles with antibodies as optical biosensing platforms.

Cited by 6 publications

References 50 publications

Specific Detection of Uropathogenic Escherichia coli via Fourier Transform Infrared Spectroscopy Using an Optical Biosensor

Specific Detection of Uropathogenic Escherichia coli via Fourier Transform Infrared Spectroscopy Using an Optical Biosensor

Experimental and molecular dynamics simulation study on antifouling performance of antimicrobial peptide‐modified aluminum alloy surfaces

The optimization of self-assembled monolayer of thiols on screen-printed gold electrode

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