Optimization, Validation and Application of HPLC-PDA Methods for Quantification of Triterpenoids in Vaccinium vitis-idaea L.

Abstract

Triterpenoids have regained much attention as promising multi-targeting bioactive agents of natural origin in the treatment of numerous disorders. Due to the high potential for phytopharmaceutical development, accurate qualitative and quantitative analysis of triterpenoids for screening and quality control is required. Vaccinium vitis-idaea L. (lingonberry) raw materials have aroused interest as a rich source of triterpenoids. However, currently, no validated, rapid, and easy-to-perform quantification method is available for the routine control of these compounds in lingonberries. This research aimed at developing and validating HPLC-PDA methods for the determination and screening of triterpenoids in extracts of lingonberry leaves, fruits, and flowers. The developed methods were deemed satisfactory by validation, which revealed acceptable analytical specificity, linearity (r2 > 0.9999), precision (RSD < 2%), trueness (94.70–105.81%), and sensitivity (LOD: 0.08–0.65 µg/mL). The real sample analysis demonstrated established methods applicability for quantification of 13 triterpenoids in lingonberries and emphasized differences between raw materials. Lingonberry fruits were distinguished by the richness of ursolic acid; lingonberry flowers by similar profile to fruits, but low content of neutral triterpenoids; whereas lingonberry leaves by the particularly high level of α-amyrin. Thus, the proposed methods proved to be reliable and applicable for quantification and routine analysis of triterpenoids in lingonberry samples.

Saponin‐rich fractions from Codonopsis javanica root extract and their in vitro antioxidant and anti‐enzymatic efficacy

“…In this study, by using HPLC‐UV, a packed C18 column, and a mixture of acetonitrile and methanol, one triterpenoid saponin, namely OA ( t R = 13.876 min), was identified from the four fractions and CE from C. javanica roots (Figure 1). OA was also found in some previous studies (Lee et al., 2018; Vilkickyte & Raudone, 2021; Xu et al., 2014; Zhang, Xue, et al., 2013). The concentration of OA was calculated based on the standard calibration curves at a detection wavelength of 210 nm, that is, y = 1.0065 x + 10.25 ( R 2 = 0.9855).…”

Sea Buckthorn Leaf Powders: The Impact of Cultivar and Drying Mode on Antioxidant, Phytochemical, and Chromatic Profile of Valuable Resource

“…Triterpenic compounds were analyzed using the Waters e2695 Alliance system, (Waters, Milford, MA, USA), applying the methods of Vilkickyte et al [ 60 ]. ACE C18 (150 × 4.6 mm, 3 µm) column (ACT, Aberdeen, UK) column was used and the injection volume was 10 µL.…”

Vaccinium vitis-idaea L. Fruits: Chromatographic Analysis of Seasonal and Geographical Variation in Bioactive Compounds

“…Identification was performed at a wavelength of 205 nm by comparing retention times to those of standard compounds and linear regression models were obtained for quantification purposes. Validation data of chromatographic methods presented above are given in our previous report [29].…”