Optimization studies on production of a salt-tolerant protease from Pseudomonas aeruginosa strain BC1 and its application on tannery saline wastewater treatment

Sivaprakasam, Senthilkumar; Dhandapani, Balaji; Surianarayanan, M.

doi:10.1590/s1517-83822011000400038

Cited by 26 publications

(13 citation statements)

References 11 publications

(11 reference statements)

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“…In addition, a salt tolerant protease from P. aeruginosa isolated from tannery saline effl uent was also used for wastewater treatment. Protease activity was observed at salt concentrations from 4 to 7 % (Sivaprakasam et al 2011 ).…”

Section: Treatment Of Saline Wastewatersmentioning

confidence: 99%

Halophilic Bacteria: Potentials and Applications in Biotechnology

Mohammadipanah

Hamedi

Dehhaghi

2015

Sustainable Development and Biodiversity

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Halophilic bacteria are categorized based on their requirements to salt. They apply some strategies in adaptation to the high saline environment. Their low nutritional requirements and resistance to high concentrations of salt, introduce as potent agents in wide range of biotechnological applications. Halophilic bacteria are very divergent and more than 150 species introduce in 70 genera of halophilic bacteria are reported. Use of resistant microorganisms and their metabolites under harsh industrially conditions is a strikingly important subject. Various halophilic enzymes in different enzymatically processes, compatible solutes as macromolecule stabilizers, biopolymers, biofertilizers and pharmaceutically active molecules from halophilic bacteria are among the important applications of these group. Additionally, they have many potential in bioremediation of various organic and inorganic pollutants from environment. This chapter covers the different aspects of potential applications of halophilic bacteria in biotechnology. Keywords IntroductionMicrobial life exists in hypersaline habitat refl ects the adaption of microorganisms to high concentration of salts. Halophilic and halotolerant organisms are found in all three domains of life: Archaea , Bacteria , and Eukarya . According to the requirement

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Section: Treatment Of Saline Wastewatersmentioning

confidence: 99%

Halophilic Bacteria: Potentials and Applications in Biotechnology

Mohammadipanah

Hamedi

Dehhaghi

2015

Sustainable Development and Biodiversity

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“…The ANFL was dispersed in water byusing Ultra sonicator at 23 KHz. The optimization studies were carried out with minimal media of volume 50 ml and culture broth, 2 ml at different incubation time (12-72 h), pH (3)(4)(5)(6)(7)(8)(9)(10), temperature (20-60°C), ANFL (0.5-5% w/v) and the salinity (0.5-5%) for the production of amylase [29][30][31]. Biomass in the reaction mixture was separated using refrigerated centrifuge followed by acetone precipitation.…”

Section: Isolation Of Halophilic Organism and The Amylase Productionmentioning

confidence: 99%

“…Soaking process is commonly carried out in a soaking pit or paddle drum with agitation. The soak wastewater also known as soak liquor is characterised predominantly by dissolved bioorganic compounds and inorganic compounds, high concentration of suspended solids and halophilic and halotolerant microorganisms [7][8][9][10]. The soak liquor contains bioorganic compounds (majorly proteins, lipids, and glycosaminoglycans) besides chlorides in the range 40000-80000 mg/L.…”

Section: Introductionmentioning

confidence: 99%

Removal of glycosaminoglycans present in tannery saline soak wastewater using integrated biological reactor and amylase immobilised reactor

Maharaja¹,

Priyadharshini²,

Akshaya³

et al. 2019

DWT

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The present investigation offers the scope for the elimination of glycosaminoglycans (GAGs), the amino sugars, present in tannery saline wastewater (also known as soak liquor) generated during the soaking of raw materials for the removal of common salt. The extracted salt tolerant amylase from Providencia Rettgerri sp., had very high amylase activity, 1320 U/ml. The modified NPAC(m-NPAC) was effectively produced by covalent incorporation method using ethylenediamine/glutaraldehyde for the immobilisation of amylase. The salt tolerant amylase immobilised nanoporous activated carbon (Am-NPAC) was used for the elimination of GAGs from soak liquor having chlorides of 4-5% (w/v). Various factors such as time, 120 min; pH, 6; temperature, 30°C; amylase concentration, 250 Units/g of m-NPAC and salinity, 3-5% of the medium that favour the maximum immobilisation of amylase onto m-NPAC were optimized. The instrumental techniques such as FT-IR spectroscopy, TGA-DSC, SEM and XRD analyses have confirmed the immobilisation of amylase. The optimum conditions such as time, 1 h; pH, 6; and temperature, 40°C for the elimination of GAGs from soak liquor were investigated. The amylase immobilised nanoporous carbon matrix (Am-NPAC) was very effective for the cleavage of GAGs into glucuronic acid and glucosamine bio-molecules. The removal of GAGs was confirmed through FT-IR, UV-visible and fluorescence spectroscopies.

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“…The protease retained * 50% activity in the presence of 1-2 mol/L NaCl (Table 4). A halotolerant protease from Pseudomonas aeruginosa BC1 has optimum activity at 2% (w/ v) of NaCl [32]. Also, Bacillus sp.…”

Section: Effect Of Salts On Protease Activity and Stabilitymentioning

confidence: 99%

Detergent‐Compatible Robust Alkaline Protease from Newly Isolated Halotolerant Salinicoccus sp. UN‐12

Mokashe

Chaudhari

Patil

2017

J Surfact & Detergents

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Currently, enzyme‐containing blended detergent preparations are favored in the detergent industry. The detergent compatibility of an enzyme depends on its robustness to withstand harsh operating conditions and maintain efficient functioning in the presence of various surfactants and detergents. Alkaline proteases from halotolerant microbes having the capability to work in demanding environments are desirable for their application in the detergent industry. The protease secreted by a halotolerant soil isolate Salinicoccus sp. UN‐12 was evaluated for its suitability in detergent formulations. The studied enzyme is an alkaline serine protease (63 kDa) with an optimum temperature of 55 °C and optimum pH of 8.5. The protease was purified 17‐fold with a 4% yield. The protease was active in NaCl (up to 4 mol) and showed enhanced activity in the presence of CaCl2, KCl, and MnCl2. In addition, the proteolytic activity was sustained in the presence of various organic solvents, cyclodextrin, disodium cocoamphodiacetate, ionic liquids, hydrotropes, and ultrasound. This report is the first to document the multifarious robustness and novel attributes of a protease secreted by the newly isolated Salinicoccus sp. UN‐12. The protease‐detergent formulation efficiently removes blood, egg, grass, and tomato sauce stains. This protease is now available for efficient and eco‐friendly applications in the detergent industry.

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Optimization studies on production of a salt-tolerant protease from Pseudomonas aeruginosa strain BC1 and its application on tannery saline wastewater treatment

Cited by 26 publications

References 11 publications

Halophilic Bacteria: Potentials and Applications in Biotechnology

Halophilic Bacteria: Potentials and Applications in Biotechnology

Removal of glycosaminoglycans present in tannery saline soak wastewater using integrated biological reactor and amylase immobilised reactor

Detergent‐Compatible Robust Alkaline Protease from Newly Isolated Halotolerant Salinicoccus sp. UN‐12

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