In this paper, a 1:21 model experiment was conducted to discuss the dust dispersion efficiency and liner trolley obstruction effect inside the tunnel at -9° to 9°, the effect of different initial dust concentrations on dust dispersion and liner trolley obstruction effect at 6° slope, and the effect of different return air velocity on dust dispersion at 6° slope. The results show that as the slope of the tunnel changes from 0° to -9°, the average dust dispersion time decreases by 3.7% at the working face and the dust concentration difference between the front and rear of the trolley is improved by 2.7%. When the slope of the tunnel changes from 0° to -9°, the average dust dispersion time increases by 7.2% at the working face and the dust concentration difference between the front and rear of the trolley is improved by 17.9%. With each 100mg/m3 increase in the initial dust concentration, the dust dispersion time at the working face and the tunnel exit increases by 9.15% and 8.17% on average, and the lining trolley obstruction time increases by 23.33s on average. The dust dispersion times take an average reduction rate of 15.7%, with the increase of return air velocity. The recommended return air velocity is greater than 1m/s for large slope tunnels. When the slope changes from 0°to 9°, the hindrance rate of slope on dust dispersion is 2.88462%, 8.65385%, and 16.34615% respectively. Dust dispersion efficiency will be reduced as the tunnel slope changes from 0°to 9°, The growth rate of slope on dust dispersion is -0.96154%, -2.88462%, and -6.73077% respectively.