Optimization, purification, characterization and antioxidant activity of an extracellular polysaccharide produced by Paenibacillus polymyxa SQR-21

Raza, Waseem; Makeen, Kousar; Wang, Yang; Xu, Yangchun; Shen, Qirong

doi:10.1016/j.biortech.2011.02.033

Cited by 99 publications

(49 citation statements)

References 32 publications

(47 reference statements)

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“…It was previously reported that the production of EPS was associated with cell growth (Raza et al, 2011;Wang et al, 2011;Liang et al, 2014). In the present study the bacterial growth (2.093 OD at 600 nm) was maximum after 36 h of incubation and EPS production (1.9 mg/ml) was high after 72 h incubation at 37 °C.…”

Section: Resultssupporting

confidence: 64%

“…the physical factors and nutrient components were optimized. Many researchers have evaluated the effects of process parameters on the maximal production of EPSs to optimize the process conditions, such as temperature, pH and medium composition (Rafigh et al, 2014;Raza et al, 2011). Studies of EPS have demonstrated that the composition of the culture medium plays an important role in EPS production (Liu et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…The genus Paenibacillus consists of more than 89 species of neutrophilic, facultative anaerobes, periflagellated heterotrophic, endospore forming, and low G + C content, Gram +ve bacilli, which were previously included within the genus Bacillus and further reclassified as a genus Paenibacillus (Ash et al, 1993). Bacteria belonging to the genus Paenibacillus have been isolated from variety of environments such as soil, water, insect larvae, food, vegetable matter, rhizospheres as well as clinical samples (Raza et al, 2011;Vijayaraghavan et al, 2016). Paenibacillus species produced a range of exopolysaccharides with diverse physiological and biotechnological functions.…”

Section: Introductionmentioning

confidence: 99%

“…EPSs has attracted great interest because of their biotechnological potential in the treatment of wastewater and various industrial processes. Microorganisms are good and cheaper sources for EPS production compared with algae or plants because of their ability to grow in cheaper nutrient media within a few days, high growth rate, ease of manipulation and lower space requirement (Raza et al, 2011). Hence, there has been an increasing interest in producing EPSs from any new microbial isolates.…”

Section: Introductionmentioning

confidence: 99%

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2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoExtracellular polymeric substances (EPSs) are produced by a wide array of bacteria for protection against aggregation, expression of virulence and adhesion. Growth associated EPS production was determined by Paenibacillus sp. in submerged fermentation. To improve the productivity and efficiency of EPS from Paenibacillus sp. the physical factors and nutrient components were optimized. The bacterial growth (2.093 OD at 600 nm) was maximum after 36 h of incubation and EPS production (1.9 mg/ml) was high after 3 days of incubation at 37 °C. EPS production was found to be maximum at pH 7.0 (2.31 mg/ml), and at 40 °C (2.49 mg/ml). Supplementation of 1% sucrose (4.30 mg/ml) supported more EPS production. Among the nitrogen sources, addition of yeast extract (0.1%) supported maximum EPS production (5.36 mg/ml). Among inorganic sources, NH 4 Cl significantly influenced on EPS production.

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Section: Resultssupporting

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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2017

Int.J.Curr.Res.Aca.Rev.

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“…The genus Paenibacillus consists of more than 89 species of facultative anaerobes, neutrophilic, endospore forming, periflagellated heterotrophic and low G + C Gram +ve bacilli, which were originally included within the genus Bacillus and then reclassified as a separate genus 8 . Bacteria belonging to this genus have been detected in a variety of environments such as soil, rhizospheres, water, vegetable matter, insect larvae and forage, as well as clinical samples 9 .…”

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confidence: 99%

Optimization, Purification and Physicochemical Characterization of Curdlan Produced by Paenibacillus sp. Strain NBR-10

Elsayed¹,

Arafat²,

Elsehemy³

et al. 2016

Biosci., Biotech. Res. Asia

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In recent years, significant progress has been done to discover a novel microbial exopolysaccharides that possess novel and highly functional properties. During our screening program for curdlan producing bacteria, 35 bacterial strains were isolated from 15 sandy soils collected from Rafha governorate, Northern Border region, Saudi Arabia. Among them isolate, NBR-10 was selected for its promising ability for curdlan production. The selected isolate was identified based on methods or results mentioned in this paper as Paenibacillus sp. For enhancing the curdlan yield produced by Paenibacillus sp. NBR-10, different culture conditions and medium compositions were optimized. It was found that, the maximum yield was obtained at 35 °C, initial pH 7 after 48 h of incubation. Also different carbon and nitrogen sources were used to improve the curdlan yield, it was indicated that sucrose and yeast extract were the best carbon and nitrogen sources respectively. Generally, optimization of the different parameters was approximately duplicated the curdlan yield from 2.34 g/l to 4.82 g/l. The precipitated curdlan dissolved in 2M NaOH exhibited high affinity to gel formation. Analysis of FT-IR, 1 H NMR and 13 C NMR spectra proved that the produced polymer by Paenibacillus sp. NBR-10 has β β β β β-(1-3)-D-glucan (curdlan) structure.

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Optimization, purification, and characterization of hydroxylamine oxidoreductase from Acinetobacter sp. Y1

Liu

Yuan

Liu

2019

Biotech and App Biochem

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Hydroxylamine oxidoreductase (HAO) is a key enzyme involved in ammonium removal pathway. To further study the enzyme, HAO was purified from heterotrophic nitrifier Acinetobacter sp. Y1 and its property was investigated. Results of single-factor experiments showed that the optimal carbon source, nitrogen source, and C/N ratio were trisodium citrate, ammonium sulfate, and 14, respectively, with incubation time of 16 H. DEAE Sefinose TM FF anion-exchange chromatography was used to purify HAO, followed by Sefinose TM CL-6B gel filtration chromatography. SDS-PAGE revealed that a 47 kDa enzyme was purified successfully, with a purification fold of 7.32 and a recovery rate of 19.40%. The optimized enzyme activity of purified HAO was tested at pH 8.0 and 30 • C. The results showed that the activity was increased by 43.78% and 25.64% in the presence of 1 mM Fe 2+ and Fe 3+ , respectively. HAO activity was increased with the increase of Na + and K + , Mn 2+ , Zn 2+ , Cu 2+ , Ca 2+ , Ba 2+ inhibited the HAO activity at three concentrations. In addition, HAO activity was activated by ethylenediaminetetraacetic acid at 0.4 mM, and a negative effect arose as the dose increased.

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Optimization, purification, characterization and antioxidant activity of an extracellular polysaccharide produced by Paenibacillus polymyxa SQR-21

Cited by 99 publications

References 32 publications

Untitled

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Optimization, Purification and Physicochemical Characterization of Curdlan Produced by Paenibacillus sp. Strain NBR-10

Optimization, purification, and characterization of hydroxylamine oxidoreductase from Acinetobacter sp. Y1

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