Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats

Burlamaqui, Idália Maria Brasil; Dornelas, Conceição Aparecida; Escalante, Rodrigo Dornenfeld; Mota, Daniel Magalhães Coutinho; Mesquita, Francisco José Cabral; Carvalho, Eduardo Rebouças de; Veras, Lara Burlamaqui; Rodrigues, Lusmar Veras

doi:10.1590/s0102-86502010000200005

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…After eight weeks we sacrificed the animals and carried out histological analysis (haematoxylin and eosin) of specimens from the the areas of rectal and bladder anastomosis; The colons was fixed and then stained with 0.1% methylene blue for evaluation of aberrant crypt foci in stereomicroscopy 5,6 .…”

Section: Methodsmentioning

confidence: 99%

Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

Dornelas

Santos

Correia

et al. 2016

Rev. Col. Bras. Cir.

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Objective: to evaluate the effect of L-lysine in the bladder and intestinal epithelia in rats submitted to vesicosigmoidostomy. Methods: we divided forty Wistar rats into four groups: group I -control group (Sham); group II -submitted to vesicosigmoidostomy and treated with L-lysine 150mg/kg; group III -submitted only to vesicosigmoidostomy; and group IV -received L-lysine 150mg/kg. After eight weeks the animals were sacrificed. Results: in the bladders of all operated animals we observed simple, papillary and nodular hyperplasia of transitional cells, transitional cell papillomas and squamous metaplasia. As for the occurrence of aberrant crypt foci in the colons of operated animals, we did not observe statistically significant differences in any of the distal, proximal and medium fragments, or in all fragments together (p=1.0000). Conclusion: Although statistically there was no promotion of carcinogenesis in the epithelia of rats treated with L-lysine in the observed time, it was clear the histogenesis of bladder carcinogenesis in its initial phase in all operated rats, this being probably associated with chronic infection and tiny bladder stones.

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Section: Methodsmentioning

confidence: 99%

Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

Dornelas

Santos

Correia

et al. 2016

Rev. Col. Bras. Cir.

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“…A stereomicroscope was used to for counting ACF 5,10 . along its entire length, per segment of the colon (distal, middle and proximal)…”

Section: Microscopymentioning

confidence: 99%

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

Santos¹,

Coelho

Azevedo

et al. 2016

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of L-lysine on the intestinal and urothelial epithelium of rats subjected to ureterosigmoidostomy (new model for surgical carcinogenesis). METHODS:Forty-two rats, 9 weeks of age, were divided into 6 groups. Animals in groups A, B, C were subjected to ureterosigmoidostomy (US) and treated with L-lysine, celecoxib and H 2 O, respectively. Groups D, E and F (non-operated controls) received L-lysine, celecoxib and H 2 O, respectively. The L-lysine dose was 150 mg/kg and that of celecoxib was 20 mg/kg. The colon was analyzed for the presence of aberrant crypt foci (ACF) under a stereomicroscope.The tissue was stained with hematoxylin and eosin and PAS alcian blue. RESULTS:There were rare ACF, and there was no statistically significant difference between the groups. Histopathologic study of the ureteral epithelium identified moderate to severe urothelial hyperplasia in rats with ureterosigmoidostomy. Transitional hyperplasia in the ureters of animals receiving L-lysine (A) showed an apparent difference compared to the control (C) (P=0.2424). There was no dysplasia or atypia.CONCLUSION: L-lysine does not promote carcinogenesis of the intestinal and urethelial epithelium of rats subjected to ureterosigmoidostomy at the doses and times studied.

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“…The number of ACF was higher in the distal colon (43.17 ± 16.46) compared to the proximal colon (2.33 ± 3.83) and middle colon (23.83 ± 18.00). The incidence of ACF is higher in the distal segment of the colon 8,25 . Our data indicated that the highest number of ACF was in the distal segment of the colon.…”

Section: Discussionmentioning

confidence: 96%

The role of TNF-α and NFkβ in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine

Barreira,

Campelo,

Rebouças

et al. 2023

Acta Cir. Bras.

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Purpose: To analyze the potential of tumor necrosis factor-α (TNF-α) and factor nuclear kappa B (NF-κB) as colorectal cancer (CRC) biomarkers in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine (1,2-DMH). Methods: Twenty-four male Wistar rats were divided into two groups: sham and 1,2-DMH. First, 1,2-DMH (20 mg/kg/week) was administered for 15 consecutive weeks. In the 25th week, proctocolectomy was conducted. Histopathological analysis, immunohistochemistry, and gene expression of TNF-α and NF-κB were performed. Statistical analysis was performed using GraphPad Prism. The location of aberrant crypt foci (ACF) was analyzed by Kruskal-Wallis’ test. For analyses with two groups with parametric data, the t -test was used; for non-parametric data, the Mann-Whitney’s test was used. P < 0.05 was considered significant. Results: The number of ACF and macroscopic lesions was significantly higher (p < 0.5) in the 1,2-DMH group compared to the sham group, and most ACF were concentrated in the distal segment of the colon. There was a statistically significant increase (p < 0.5) in protein and gene expression of TNF-α and NF-κB in the 1,2-DMH group compared to the sham group. Conclusions: Our results provide supportive evidence that TNF-α and NF-κB pathways are strongly involved in CRC development in rats and might be used as early biomarkers of CRC pathogenesis in experimental studies.

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Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats

Abstract: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.

Cited by 3 publications

References 11 publications

Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

Bladder carcinogenesis in rats subjected to ureterosigmoidostomy and treated with L-lysine

Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine

The role of TNF-α and NFkβ in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine

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