Optimization of various physiochemical parameters to enhance production of secondary metabolite from soil actinomycetes against dermatophytes

Abstract

The main objective of the study is to isolates actinomycetes from the Gwalior region and improves the production of secondary metabolite and cell growth of actinomycetes. Total 70 actinomycetes were isolated and screened for maximum secondary metabolites production against the dermatophytes on the bases of agar diffusion method & disc diffusion method. Out of 70 isolate, AP-27 has a good potential to produced secondary metabolite that inhibit the growth of dermatophytes (Microsporum gypseum, Microsporum fulvum, Tricophyton rubrum, and Tricophyton mentagrophytes). The optimization of several growth parameters like-growth medium, Incubation time, carbon sources, nitrogen sources, temperature, pH and minerals for the maximum production of secondary metabolites were checked. The optimum growth of AP-27 occurred with starch casein broth containing 2% starch as carbon source and nitrogen source was potassium nitrate nitrate at 2% at pH 7 and temperature 30⁰C. Soybean meal was also found to be the best nitrogen source after the potassium nitrate which can help in large scale production because In India 74% of soyabean is produced by Madhya Pradesh. FeSO 4 (0.01gm/100ml) was selected as optimum mineral source. It was noticed that 6 days old culture was showing the maximum zone of inhibition and cell growth.

Antifungal activity of Streptomyces canescens MH7 isolated from mangrove sediment against some dermatophytes

“…Among various carbon and nitrogen sources starch and potassium nitrate were selected as best carbon and nitrogen sources for secondary metabolite production. Previous results for optimized C source and N source corresponded with our results [69].…”

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

“…The differences obtained in the synthesis of antifungals and biopigments shown by Streptomyces spp. in ISP2 and PW media could be associated with differences in microbial growth rates [ 53 ] and/or the components of the nutritional support [ 24 ]. The PW is composed of damaged potato tubers, which have a high starch content between 60% and 70% on a dry basis [ 54 ].…”

“…The PW is composed of damaged potato tubers, which have a high starch content between 60% and 70% on a dry basis [ 54 ]. Among the carbon sources, starch is one of the most effective nutritional supplements for increasing antibiotic production [ 24 ]. Therefore, its nutritional composition makes PW a rich source of starch for the synthesis of different antibiotic compounds.…”

“…The authors concluded that the presence of starch in the culture medium enhanced the production of antibiotics. In this way, the synthesis of bioactive compounds has been related to different parameters of the nutritional support, including carbon and nitrogen sources [ 24 ]. Long et al [ 25 ] also reported a relationship between biopigment production by Monascus ruber and starch hydrolysis.…”