Optimization of Ultrasound-Assisted Extraction, Preliminary Characterization and In Vitro Antioxidant Activity of Polysaccharides from Green Pea Pods

Safaryan, Maryam Jalili; Ganjloo, Ali; Bimakr, Mandana; Zarringhalami, Soheila

doi:10.3390/foods5040078

Cited by 30 publications

(17 citation statements)

References 45 publications

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“…If the ultrasonication extraction time continued increasing from 45 min to 75 min, the antioxidant ability of the GMBC extraction showed a decreasing trend. The antioxidant ability increased with the extracting time at first and then decreased after the optimal extraction time, which showed a similar trend to the studies about Pisum sativum [ 24 ] and Codonopsis pilosula [ 25 ]. Apparently, the best extraction time for GMBC was 45 min in this study.…”

Section: Resultssupporting

confidence: 85%

Optimization of Ultrasound-Assisted Extraction of Antioxidants from the Mung Bean Coat

et al. 2017

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Mung bean (Vigna radiata) sprout is commonly consumed as a vegetable, while the coat of the germinated mung bean is a waste. In this paper, an ultrasound-assisted extraction method has been developed to extract natural antioxidants from the seed coat of mung bean. Several experimental parameters—which included ethanol concentration, solvent/material ratio, ultrasound extraction time, temperature, and power—were studied in single-factor experiments. The interaction of three key experimental parameters (ethanol concentration, solvent/material ratio, and ultrasonic extraction time) was further investigated by response surface method. Besides, traditional extracting methods, including maceration and Soxhlet extraction methods, were also carried out for comparison. The results suggested that the best extracting condition was 37.6% (v/v) of ethanol concentration, 35.1:1 mL/g of solvent/material ratio and ultrasonic extraction of 46.1 min at 70 °C under 500 W ultrasonic irradiation. The antioxidant capacity (178.28 ± 7.39 µmol Trolox/g DW) was much stronger than those obtained by the maceration extraction process (158.66 ± 4.73 µmol Trolox/g DW) and the Soxhlet extraction process (138.42 ± 3.63 µmol Trolox/g DW). In addition, several antioxidant components in the extract were identified and quantified. This study is helpful for value-added utilization of the waste from germinated mung bean.

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Section: Resultssupporting

confidence: 85%

Optimization of Ultrasound-Assisted Extraction of Antioxidants from the Mung Bean Coat

et al. 2017

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“…In recent advances through diet, due to increase in phytochemical, there is scope of enhancement of functional or designer food which would help in prevention of numerous diseases (Burguieres et al, 2008). Green pea pod polysaccharide (GPPP) was extracted through an ultrasound extraction technique that showed an appreciable amount of scavenging activity (91.03%) by the free radicals obtained from DPPH assay (JaliliSafaryan et al, 2016). It has a reducing power of 63% and also contains ferric reducing antioxidant power of 0.34 mmol/L which is obtained from a total amount of 0.9mg/mL.…”

Section: Phytochemicals and Antioxidantsmentioning

confidence: 99%

Potential health benefits of garden pea seeds and pods: A review

Kumari

Deka

2021

Legume Science

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Pea (Pisum sativum L.) is a nutritious pulse crop belonging to the leguminous family, and widely cultivated. In recent time, health and nutritional benefits of the pea and its by-products enriched with biomolecules, which have gained significant attention to the researchers and consumers. Peas are known for its pivotal nutritional value with high-quality proteins, dietary fibres, starch, carbohydrates and micronutrients including vitamins and minerals. It is also enriched with phytochemicals, antioxidants, flavonoids, tannins and other phenolic compounds under nonnutritional category. Dietary fibre extracted from its cotyledon of the cell wall, seed coats and pods proved to accelerate the gastrointestinal activity by developing intestinal microflora. Intermediate amylose maintains blood glucose level by lowering the glycaemic index and reducing starch digestibility of peas. Pea proteins derived peptides have opportunities in nutraceuticals. Furthermore, pea-derived non-α-galactooligosaccharides could mitigate the gas production by colonic bacteria during the fermentation. Anti-acetylcholinesterase (anti-AChE) was also traced in the pea pod with some antioxidant properties. The focus of this review paper is to provide a comprehensive profiling of chemical compositions and bioactives of fresh mature peas (specifically garden or field pea seeds) and by-products of pea processing (pods and peels) that sets a foundation for assessment of their health benefits. K E Y W O R D Sby-products, human health, nutritional composition, pea proteins, phenolics), a nutritious agricultural commodity belongs to the Leguminosae family, has potential to withstand freezing temperature which is widely cultivated across the globe (Kour et al., 2020;Tulbek et al., 2017). In terms of productivity, India stands as the second largest producer of green peas next to China and ranks ten among the vegetable crops. The annual global production of green pea and dry pea seeds are approximately 14.5 million tons and 22 million tons, respectively (FAOSTAT, 2019;Mondor, 2020;Senapati et al., 2019). Pea seeds are well known for its pivotal nutrients that can be differentiated in mainly soluble and insoluble fibres, proteins (tryptophan and lysine), complex carbohydrates, vitamin B, folate, minerals namely, calcium, potassium, iron but low content of saturated fat, cholesterol and sodium (Figure 1). Fresh green pea seeds contain 17-22g carbohydrates, 20-to 50-g starch, 14-to 26-g dietary fibre, 6.2-to 6.5-g protein, 0.4-g fat, 1.0-g ash per 100 g with 9-to 10-mg calcium, 3-to 5-mg sodium, 97-to 99-mg potassium per

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“…It has been reported that a water-to-raw material ratio rose above 25 ml/g, the polysaccharide yield started to decline slowly 21 . A possible explanation is that the increase of water-to-raw material ratio may increase diffusivity of the solvent into cells and enhance the desorption of the polysaccharide from the cells 22,23 . Thus, water-to-raw material ratio was set in the range of 25–35 mL/g for optimization design (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

The structural characterization of a polysaccharide exhibiting antitumor effect from Pholiota adiposa mycelia

Zou

et al. 2019

Sci Rep

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PAP80-2a, purified from Pholiota adiposa mycelia, is a polysaccharide exhibiting prominent antitumor effects. However, the yield of PAP80-2a was low and its structure has not been characterized, impeding the exploration of its structure-function relationship, thus influencing the development of oral drugs for antitumor therapy and immunomodulation. In order to improve the yield of PAP80-2a, response surface methodology along with Box-Behnken design was applied to optimize the ultrasonic-assisted extraction conditions for polysaccharides. Then, the structure of PAP80-2a exhibiting antitumor activity was determined from different angles. The results showed that the extraction yield of P. adiposa polysaccharides increased by 11.5% under optimized ultrasonic extraction conditions. Structural analysis showed that PAP80-2a was mainly composed of glucose, rhamnose, xylose, and galactose in a ratio of 10.00: 2.09: 4.09: 1.13. The total amino acid content in the sugar chain was 69.92 μg/mL. The sugar chain structure was [α-Rha (1 → 3)-]n, and rhamnose was located at the non-reducing end of the sugar chain, while glucose was located at the non-reducing end or in the sugar chain in 1,2,6- and 1,3,6-linked forms. Our study clearly illuminates the primary structure of PAP80-2a, but 3D structure of PAP80-2a and its structure–function relationship is a future challenge.

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Optimization of Ultrasound-Assisted Extraction, Preliminary Characterization and In Vitro Antioxidant Activity of Polysaccharides from Green Pea Pods

Cited by 30 publications

References 45 publications

Optimization of Ultrasound-Assisted Extraction of Antioxidants from the Mung Bean Coat

Optimization of Ultrasound-Assisted Extraction of Antioxidants from the Mung Bean Coat

Potential health benefits of garden pea seeds and pods: A review

The structural characterization of a polysaccharide exhibiting antitumor effect from Pholiota adiposa mycelia

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