Optimization of ultrasound-assisted extraction of bitter compounds and polyphenols from willow gentian underground parts

Jovanović, Maja; Mudrić, Jelena; Drinić, Zorica; Matejić, Jelena S.; Kitić, Dušanka; Bigović, Dubravka; Šavikin, Katarina

doi:10.1016/j.seppur.2021.119868

Cited by 19 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is evident that the most dominant factor influencing TPC extraction was extraction temperature, and this factor affected TPC positively. This is consistent with the findings of the aforementioned study [ 26 ], where the temperature was also the most influential factor affecting TPC extraction. High extraction temperatures enhance the solubility and diffusion of polyphenols [ 33 ].…”

Section: Resultssupporting

confidence: 93%

“…The extraction yield of TPC from the underground parts of willow gentian ranged from 10.55 to 13.18 mg GAE/g DW. The results obtained were slightly higher compared to those obtained by UAE using water-ethanol as a solvent [ 26 ]. According to them, the extraction yield of TPC from the underground parts of the willow gentian varied from 5.64 to 10.26 mg GAE/g DW.…”

Section: Resultsmentioning

confidence: 67%

“…Taking into account that the phytochemical profile of extracts depends on numerous parameters, such as power and frequency of the ultrasound, the type of solvent, solid-to-liquid ratio, extraction time, particle size, temperature, and presence of co-solvents extraction, optimization of operating conditions is a rational approach to provide a highly efficient process [ 18 , 26 ]. The traditional “one-factor-at-a-time” optimization approach is time- and material-consuming and inefficient for evaluating the interaction effect of process parameters.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

et al. 2022

Self Cite

View full text Add to dashboard Cite

The residue after sieving (“dust”) from the willow gentian underground parts is an unexploited herbal tea by-product, although it contains valuable bioactive compounds. Cyclodextrins as efficient green co-solvents, cage molecules, and multifunctional excipients could improve the extraction and contribute to the added value of the resulting extracts. The objective of this study was to determine the optimal conditions for the extraction of gentiopicroside, isogentisin, and total phenolics (TPC) from willow gentian “dust” using ultrasound-assisted water extraction coupled with hydroxypropyl-β-cyclodextrin (HPβCD). The influence of extraction temperature (X1: 20–80 °C), time (X2: 20–50 min), and HPβCD concentration (X3: 2–4% w/v) was analyzed employing the response surface methodology (RSM). The optimal extraction conditions for simultaneously maximizing the extraction yield of all monitored responses were X1: 74.89 °C, X2: 32.57 min, and X3: 3.01% w/v. The experimentally obtained response values under these conditions (46.96 mg/g DW for gentiopicroside, 0.51 mg/g DW for isogentisin, and 12.99 mg GAE/g DW for TPC) were in close agreement with those predicted, thus confirming the suitability and good predictive accuracy of the developed RSM models. Overall, the developed extraction system could be an applicable alternative strategy to improve the extraction of bioactive compounds from the underutilized “dust” of willow gentian underground parts.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The extraction yield of plants is affected by numerous factors, including extraction time, extraction temperature, solvent type, particle size and liquid-solid ratio [50]. In this experiment, four factors, namely ethanol concentration, extraction temperature, extraction time, and liquid-solid ratio, which greatly influence the effect of the polyphenols extraction rate from medicinal materials, were selected as variables [51]. As mentioned previously, the particle size affects the extraction yield [52].…”

Section: Determination Of Polyphenol Contentmentioning

confidence: 99%

Optimization of Ultrasound-Assisted Extraction of Polyphenols from Ilex latifolia Using Response Surface Methodology and Evaluation of Their Antioxidant Activity

et al. 2022

View full text Add to dashboard Cite

The polyphenolic extract of Ilex latifolia (PEIL) exhibits a variety of biological activities. An evaluation of the parameters influencing the ultrasonic extraction process and the assessment of PEIL antioxidant activity are presented herein. Response surface methodology (RSM) was used to optimize the experimental conditions for the polyphenols ultrasonic-assisted extraction (UAE) from the leaves of Ilex latifolia. We identified the following optimal conditions of PEIL: ethanol concentration of 53%, extraction temperature of 60 °C, extraction time of 26 min and liquid–solid ratio of 60 mL/g. Using these parameters, the UAE had a yield of 35.77 ± 0.26 mg GAE/g, similar to the value we predicted using RSM (35.864 mg GAE/g). The antioxidant activity of PEIL was assessed in vitro, using various assays, as well as in vivo. We tested the effects of various doses of PEIL on D-galactose induced aging. Vitamin C (Vc) was used as positive control. After 21 days of administration, we measured superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, malondialdehyde (MDA) levels in mouse serum and liver tissue. The results demonstrated that the PEIL exhibits potent radical scavenging activity against 1,1-diphenyl-2-picrythydrazyl (DPPH∙), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+), and hydroxyl (∙OH) radicals. The serum concentrations of SOD and GSH-Px were higher, and MDA levels were lower, in the medium- and high-dose PEIL-treated groups than those in the aging group (p < 0.01), and the activity of MDA was lower than those of the model group (p < 0.01). The liver concentrations of SOD and GSH-Px were higher (p < 0.05), and MDA levels were lower, in the medium- and high-dose PEIL-treated groups than those in the aging control group (p < 0.01). These results suggest that optimizing the conditions of UAE using RSM could significantly increase the yield of PEIL extraction. PEIL possesses strong antioxidant activity and use as a medicine or functional food could be further investigated.

show abstract

“…To overcome these restrictions NaDES are often integrated with accelerating extraction techniques, such as ultrasound-assisted extraction (UAE). Namely, ultrasound through the cavitation bubble collapse generates a strong shear force and “hot spots” of extremely high temperature that reduce solvent viscosity but also the fragmentation of plant material and facilitate solvent penetration [ 16 , 17 ]. Additionally, ultrasound disrupts the structure of the plant matrices and intensifies the mass transfer [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability

Jovanović

Krgović

Živković

et al. 2022

Plants

View full text Add to dashboard Cite

Bilberry fruits (Vaccinium myrtillus L.) are one of the richest natural sources of anthocyanins and are widely used due to their pharmacological and nutritional properties. To ensure their maximum application potential, it is necessary to overcome the limitations of conventional extraction solvents and techniques. This study aimed to develop a green method for bilberry anthocyanin extraction using natural deep eutectic solvents (NaDES) integrated with ultrasound-assisted extraction (UAE) in order to define extraction conditions that will prevent decomposition of the anthocyanins or the loss of bioactivity. After a screening of ten different NaDES, choline chloride:sorbitol (1:1) was selected as the most effective. Furthermore, the influence analysis and optimization of the NaDES–UAE extraction conditions were carried out employing response surface methodology. The optimal conditions were found to be an extraction time of 37.63 min, a temperature of 48.38 °C, and 34.79% (w/w) water in NaDES. The extraction yields of target compounds under optimized extraction conditions were 0.27 mg/g DW of cyanidin-3-O-glucoside and 2.12 mg CGE/g DW of TAC. The obtained optimized extract showed promising radical scavenging and antimicrobial activity. A stability study with the optimized extract revealed that refrigerated storage at 4 °C in the dark provided the best anthocyanins preservation. Overall, the developed NaDES-UAE method showed promising application potential and can be considered as a high-efficiency green alternative to conventional anthocyanins extraction methods, enabling the preservation of active ingredients and the bioactivity of extracts.

show abstract

Optimization of ultrasound-assisted extraction of bitter compounds and polyphenols from willow gentian underground parts

Cited by 19 publications

References 21 publications

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules

Optimization of Ultrasound-Assisted Extraction of Polyphenols from Ilex latifolia Using Response Surface Methodology and Evaluation of Their Antioxidant Activity

Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability

Contact Info

Product

Resources

About