Optimization of ultrasound-assisted extraction of protein from egg white using response surface methodology (RSM) and its proteomic study by MALDI-TOF-MS

Abstract

The presented work proposes an approach based on proteomic techniques to identify proteins in egg white. Response surface methodology (RSM) with Box-Behnken design (BBD) was used to optimize the ultrasound-assisted extraction process of proteins from the egg white. The effects of extraction parameters, including trifluoroacetic acid (TFA) concentration, ultrasonic power and ultrasonic time on the extraction process of proteins were evaluated. The optimized extraction parameters were trifluoroacetic acid (TFA) concentration, 0.53% (v/v); ultrasonic power, 50% and ultrasonic time, 40 min. The effects of three factors (trypsin volume, pH of tryptic digestion and incubation time) on the tryptic digestion process of extracted proteins were investigated. The results showed that the optimized tryptic digestion conditions were as follows: volume of 10 mg mL À1 trypsin, 8 mL; pH, 7.0 and incubation time, 16 h. Under the optimized conditions, the peptide mixture was analysed using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). The methodology was successfully applied to free-range and barn-raised chicken egg white for protein identification. The results showed that 23 proteins and 29 proteins were identified in free-range chicken and barn-raised chicken egg white, respectively. Among them, the collagen peptides were found in egg white for the first time.

Optimization of ultrasound-assisted extraction (UAE) of phenolic compounds from Crataegus pinnatifida leaves and evaluation of antioxidant activities of extracts

“…33,34 It was reported that water could be helpful to enhance swelling of plant material, while ethanol is believed to disrupt the bonding between the solutes and plant matrices. 35,36 The increase in the extractability of phenolic compounds with adding of water to ethanol can be attributed to the increase in permeability of plant tissues enabling better mass-transfer by diffusion. 37 However, high concentration of ethanol may increase the polarity of the solution, holding back the dissolution of phenolic compounds and then decreasing the extraction yield.…”

Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct

“…SNPs associating with early or late AW significantly were reported on chromosomes 1, 3, 5, 18, 19, 23 and Z by genome-wide association studies (Abasht et al, 2009). Albumen proteomic researches have greatly expanded the identified albumen proteins in various layer breeds (Guerin-Dubiard et al, 2006;Mann, 2007;D'Ambrosio et al, 2008;Mann and Mann, 2011;Wang et al, 2012;He et al, 2014). The changes of albumen protein components during the degeneration process was also reported (Omana et al, 2011).…”

Characterization of Degraded Proteins in Paintings Using Bottom-Up Proteomic Approaches: New Strategies for Protein Digestion and Analysis of Data

“…New field-specific procedures need to be developed to address these field-specific analytical challenges 15,17 . Research so far has focussed on the improvement of the solubilisation step [17][18][19][20][21][22] , and the combined use a protein deglycosidase enzyme to improve the detection of egg 23 .…”