Optimization of ultrasonic-assisted extraction via multiresponse surface for high antioxidant recovery from<i>Chlorella vulgaris</i>(Chlorophyta)

Nasir, Nur Tsiqah Binti Mohd; Şahin, Saliha; Çakmak, Zeynep Elibol; Çakmak, Turgay

doi:10.2216/16-132.1

Cited by 11 publications

(8 citation statements)

References 43 publications

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“…The antioxidant capacities of bee pollen extracts were determined with chromium reducing antioxidant capacity (CHROMAC) (Nasır et al, 2017;Şahin and Demir, 2013) and ferric reducing antioxidant power (FRAP) (Benzie and Strain, 1996) methods. In CHROMAC method, the extracts of chestnut bee pollen (100 µL), 400 µL of distilled water and 3.5 mL of phosphate buffer (pH 2.8) were taken to the test tube and the test tube was mixed.…”

Section: Antioxidant Capacity Analysismentioning

confidence: 99%

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

Karkar¹,

Şahin²,

Güneş³

2018

GIDA

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Chestnut bee pollen is a high antioxidative natural bee product. In this study the phenolic compounds in chestnut bee pollen were determined by HPLC-DAD system. Ethanol, water and methanol were used for the extraction of antioxidant compounds from chestnut bee pollen. The total phenolic contents and antioxidant capacities of extracts were determined by Folin-Ciocalteu, CHROMAC (Cr(VI) reduction antioxidant capacity) and FRAP (ferric reducing antioxidant power) methods, respectively. When compared the amounts of phenolic compounds and spectroscopic results (total phenolic contents and antioxidant capacities), the most suitable solvent was found ethanol for the extraction of antioxidant compounds from bee pollen. The major phenolic compounds in the chestnut bee pollen were determined as pinocembrin, chrysin, galangin and hyperoside with the contents of 1.246, 0.332, 0.122 and 0.516 mg/g of bee pollen, respectively. And also it was determined that the chestnut bee pollen was inhibited 11% of DNA oxidation in Fenton medium.

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Section: Antioxidant Capacity Analysismentioning

confidence: 99%

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

Karkar¹,

Şahin²,

Güneş³

2018

GIDA

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“…The antioxidant capacities of the CBPE were determined with ferric reducing antioxidant power (FRAP) (Benzine & Strain., ), chromium reducing antioxidant capacity (CHROMAC) (Nasir, Şahin, Çakmak, & Çakmak, ), and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) (Aybastıer et al, ) methods. In the FRAP method, 0.25 ml of extract and tripyridyltriazine (2.75 ml) were mixed and left to stand at 37°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases

Şahin

Karkar

2019

J Food Biochem

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Chestnut bee pollen has potential nutritional and medicinal effects and is an important natural bee product. This study focused on the investigation of the antioxidant capacity and DNA damage inhibition ability of chestnut bee pollen (CBP) from Bursa (Turkey). The phenolic compounds (rosmarinic acid, vitexin, hyperoside, pinocembrin, trans‐chalcone, apigenin, protocatechuic, and galangin) and carotenoids in CBPE were determined by HPLC‐DAD (high‐performance liquid chromatography‐diode array detection). Additionally, the protective ability of CBPE against DNA damage by oxidation was investigated. In this study, it was determined that CBPE has a high total phenolic compound content, and the antioxidant capacity of CBPE inhibits DNA oxidation (34% reduction of DNA damage in Fenton reaction media). This study could reveal new information regarding the use of CBPE as a protective agent for DNA in the future. Practical applications Phenolic compounds and carotenoids prevent some diseases because of their important biological activities. One of the potential food sources chestnut bee pollen contains sugar, carbohydrates, amino acids, proteins, lipids, vitamins, hormones, enzymes, and flavonoids. Chestnut bee pollen, which has protective activity against DNA oxidation, could be an excellent potential source of a protective agent against some degenerative diseases through future applications.

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“…The extraction parameters are given in Table 1. (Şahin et al , Nasır et al 2017. In the ABTS method, 3.8ml of ethanol was added to the C. longa sample (0.2ml).…”

Section: Ultrasound-assisted Extraction (Uae) Methodsmentioning

confidence: 99%

“…The antioxidant capacities of the extracts were determined with the chromium reducing antioxidant capacity (CHROMAC) (Işık et al 2013) and ABTS (Re et al 1999) methods with slight modifications (Şahin et al , Nasır et al 2017). In the ABTS method, 3.8ml of ethanol was added to the C. longa sample (0.2ml).…”

Section: Antioxidant Capacitymentioning

confidence: 99%

“…Non-conventional extraction techniques include ultrasound-assisted (Mandal et al 2009), supercritical fluid, pressurized liquid and microwave extractions (Mandal et al 2008). Among these techniques, ultrasound-assisted extraction (UAE) is widely used for the extraction of phytochemicals and is a simple, inexpensive, energy-saving, and efficient method when compared with other extraction techniques (Nasır et al 2017). UAE was used for the extraction of antioxidant compounds from plants with higher yields (Şahin et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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OPTIMIZATION OF ULTRASONIC-ASSISTED EXTRACTION PARAMETERS FOR ANTIOXIDANTS FROM Curcuma longa L.

Şahin

2018

Trakya University Journal of Natural Sciences

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In this study, ultrasonication was used to extract antioxidant compounds such as polyphenols, flavonoids, and curcuminoids from turmeric (Curcuma longa L.) The influences of time, ethanol concentration and temperature as three independent factors on the extraction of the total phenolic content were evaluated by the Folin-Ciocalteu method and the antioxidant capacities by the ABTS and chromium reducing antioxidant capacity (CHROMAC) methods. The central composite design (CCD) with a multi-response surface methodology (MRSM) was used for the statistical modeling of the response data followed by the regression and analysis of variance (ANOVA) to determine the significance of the model and the factors. The response predictions obtained at optimum extraction conditions of an extraction time of 64min, an ethanol concentration of 82% (v/v) and an extraction temperature of 32°C were 47.32mg GAE/g (for Folin-Ciocalteu), 29.15 (for ABTS) and 5.17mg TE/g (for CHROMAC). The predicted values obtained from the multi-response surface methodology agreed with the experimental values data 95% confidence level. These data indicate that the multi-response surface methodology is applicable for optimizing the ultrasonic-assisted extraction of antioxidant compounds from C. longa.

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Optimization of ultrasonic-assisted extraction via multiresponse surface for high antioxidant recovery fromChlorella vulgaris(Chlorophyta)

Cited by 11 publications

References 43 publications

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases

OPTIMIZATION OF ULTRASONIC-ASSISTED EXTRACTION PARAMETERS FOR ANTIOXIDANTS FROM Curcuma longa L.

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