Optimization of the ultrasound-assisted aqueous enzymatic extraction of Pinus koraiensis nuts oil by response surface methodology

Abstract

Response surfaces methodology was established in order to optimize ultrasoundassisted aqueous alkaline protease extraction parameters of Pinus koraiensis nuts oil (PNO) in this work. On the oil yield, the impacts of single factors were studied. The liquid-solid ratio, enzyme addictive quantity, enzyme hydrolysis temperature, and enzyme hydrolysis duration were chosen for further optimization of the extraction process utilizing a Box-Behnken design based on statistical significance analysis. Under ideal extraction conditions, a maximum oil recovery of 68.35% was achieved: solid-liquid ratio, enzyme concentration, enzyme hydrolysis temperature, and enzyme hydrolysis duration were 1:5 (g/mL), 3.23 mg/g, 44 °C, and 2.84 h, respectively. Furthermore, physicochemical properties testing revealed that the oil was of higher quality than other approaches. Meanwhile, the DPPH radical-scavenging activities increased with increased content compared to olive oil, with an IC 50 value of 0.082 mg/mL. The method has a lot of potential when it comes to extracting oils from plants.

Application of an aqueous enzymatic–ultrasound cavitation method for the separation of Sapium sebiferum seed kernel oil

“…4 b). This is likely due to the synergetic effects of the enzymatic hydrolysis and cavitation caused by the ultrasound waves [51] , [52] . A similar phenomenon was found in the ultrasound extraction process for mahua seed oil and Cinnamomum camphora seed oil [16] , [50] .…”

Phytohormone biosynthesis and transcriptional analyses provide insight into the main growth stage of male and female cones Pinus koraiensis

“…It has state approval to be designated as a secondary wild plant for national protection ( Li et al., 2020 ). Due to its plentiful lipid, amino acids, proteins, and other nutrients that can successfully decrease blood lipids and cholesterol in humans, P. koraiensis seed kernels have become one of the most significant nut foods accessible ( Song et al., 2021 ; Ryuk et al., 2022 ; Wang et al., 2022 ). It frequently takes the natural P. koraiensis 20 to 30 years to produce cones due to its slow growth cycle.…”