Optimization of the production of a maltooligosaccharides producing amylase from the alkalophilic Streptomyces lonarensis strain NCL 716 using SVR Modeling

Abstract

The Streptomyces lonarensis strain NCL 716 hydrolyses starch to produce a mixture of maltotriose (G3) and maltotetraose (G4) along with maltose (G2). The objective of the present work was to determine an optimum cost effective media composition for the production of α‐amylase from this strain. The most influential factor was found to be starch while the least influential factor found was peptone by Plackett–Burman method. Peptone amount was kept constant throughout the fermentation. Peptone, which is one of the expensive media components was used at a concentration of 1 g/L, which made the optimum media composition cost effective. A support vector regression‐based process model was developed for approximating the non‐linear relationship between the fermentation operating variables and the α‐amylase yield. Multicanonical Jump Walk Annealing, a stochastic optimization technique is used to obtain optimal operating variables to maximize amylase yield. The maximum amylase activity thus obtained was in good agreement with the experimental values at the optimized levels. The optimum media composition obtained by this method was: yeast extract: 4.53 g/L, starch: 20.246 g/L, K2HPO4: 0.0827%, MgSO4: 0.15%, peptone: 1 g/L. A maximum enzyme activity of 297 U/mL, which was achieved using the above approaches compares well with the activity of reported amylases producing maltooligosaccharides.

A Maltooligosaccharides Producing α-Amylase fromBacillus subtilisSDP1 Isolated from Rhizosphere ofAcacia cyanophyllaLindley

“…In accordance with the literature, in this study, yeast extract was observed to induce high amylase production of 68.06 U/mL. Sharma et al (2013) investigated the optimization of the production of amaltooligosaccharides producing amylase. Similar to our results the most influential factor was found to be yeast extract while the least influential factor found was peptone.…”

“…The manufacture of small maltooligosaccharides is not easy, and the search for microbial enzymes that specifically act on starch substrate to produce specific maltooligosaccharides has gained importance (Sharma et al, 2013). There are a few amylases (Kuddus et al, 2012) that specifically produce such maltooligosaccharides at mild conditions.…”

Foam mat drying of papaya using microwaves: Machine learning modeling

“…It uses the support vectors to define the hyperplane which maps the relationship between the response and independent variables. The SVRs minimize generalized error rather than the training error thus avoiding the problem of overfitting (Sharma et al, ). SVR has gained popularity in recent years as an alternative to the ANNs as it possesses the ability to model complex non‐linear relationships using less computational space.…”

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

“…Streptomyces spp NAA-28 Biotechnology Food, paper, detergent, fermentation, textile, and pharmaceutical sectors [51] Streptomyces griseus (SGAmy) medicine Antibiofilm agent [52] Streptomyces lonarensis NCL 716 Food Maltooligosaccharides production [53] Streptomyces erumpens MTCC 7317 Food Soluble starch and cassava starch hydrolysis [54] Streptomyces enissocaesilis NRRL B-16365 Food High fructose corn syrup production [55] Streptomyces spp Food Bakery [56] Streptomyces spp SNAJSM6 Biotechnology α-amylase production [57]…”