A Maltooligosaccharides Producing α-Amylase fromBacillus subtilisSDP1 Isolated from Rhizosphere ofAcacia cyanophyllaLindley

Abstract

Maltooligosaccharides producing amylases are required in the food industry, especially in breadmaking. The Bacillus subtilis strain SDP1 amylase hydrolyses starch to produce maltotriose and maltotetraose along with maltose after prolonged reactions of 5 h. Bacillus subtilis strain SDP1 was isolated from the rhizosphere of Acacia cyanophylla Lindley from the Çukurova region of Turkey. The highest enzyme production was achieved with soluble starch as the carbon and yeast extract as the nitrogen source and at pH 7.0 and 37 • C. Under optimized culture conditions, 68.49 U/mL activity was obtained. SDP1 α-amylase had molecular weight of 61 kD. The optimum pH of the enzyme was 7.0 and was highly active at pH ranging from 5.0 to 9.0. The optimum temperature of the crude enzyme was 60 • C, and it retained 83% and 74% of its initial activity after 1 h and 2 h incubation periods, respectively, at 50 • C. While, Mn +2 has a stimulatory effect on the activity, Ca +2 , Mg +2 , Na + did not effect the enzyme activity. Fe +3 , Ni +2 , Cu +2 and Co +2 had an inhibitory effect on SDP1 amylase activity.

A novel PLED architecture containing biologically synthesized gold nanoparticles and ultra thin silver layer

“…5). For carrier-limited materials, the quantum efficiency could be enhanced by inserting a layer that effectually transport electrons from the cathode and so this will improve the performance of optoelectronic devices [39][40][41][42][43][44][45][46][47][48][49][50].…”

“…Taxonomic characterization of strain was determined by using morphological, physiological and molecular methods such as 16S rRNA analysis [41]. The sequence has been submitted to the genbank with the accession number HM235916.…”

Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

“…The acidstable, thermostable and Ca 2+ -independent amylases reduce the cost and time required for multistep MOS production from raw starches ( Sharma et al, 2016 ). To date, several amylases have been reported with optimum pH activity at 4–7 ( Khemakhem et al, 2009 ; Asoodeh et al, 2010 ; Sharma and Satyanarayana, 2013 ; Ozturk et al, 2014 ). However, the acid stability, Ca 2+ independence, thermostability, raw starch saccharification and a very high K cat value of Ba-Gt-amy make it distinct from others.…”

Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

“…On the contrary, Rahmani et al (2013) found maltose and maltotriose produced by amylase of Brevibacterium sp. using black potato starch as substrate, while amylase of Bacillus subtilis strain SDP1 isolated from rhizosphere of Acacia produces maltotriose and maltotetraose (Ozturk et al 2014). Furthermore, Abdul-Manas et al (2014) reported that amylase of an alkaliphilic Bacillus lehensis G1 could degrade oligosaccharides by producing maltooligosaccharides with a higher degree of polymerization than maltoheptaose observed on thinlayer chromatography and high-performance liquid chromatography analyses.…”