Optimization of the magnetic bead antigen capture enzyme immuno assay for the detection of circulating anodic antigens in mixed Schistosoma infections

Novel IMB-ELISA Assay for Rapid Diagnosis of Human Toxoplasmosis Using SAG1 Antigen

“…The use of magnetic nanoparticles in immunoassay (nanomagnetic assay) combines magnetic nanoparticle high binding capacity and simple separation of bound and unbound materials as a solid phase with the rapid reaction kinetics of solutions, thus providing the chance of enhancing the sensitivity of antigen detection (71,72). The surface of the beads enables substantially more antibodies to be involved in the reaction with the antigen than in sandwich ELISA (73)(74)(75).…”

Associations Between Levels of Liver Enzymes and Circulating Antigens in Individuals Infected with Schistosomiasis Heamatobium

“…Soluble worm antigen preparation (SWAP) is considered important diagnostic target antigens, which are highly reactive with sera from schistosomiasis patients and do not cross-react with sera from patients. The magnetic bead immunoassay combines the use of magnetic beads with a high binding capacity as a solid phase and the rapid reaction kinetics of solutions with the simple separation of bound and unbound materials on the solid phase, which provides the chance of enhancing the sensitivity of antigen detection (Gundersen et al, 1992;Ndhlovu et al, 1995). The pathophysiological impact of Schistosoma mansoni infection has been mainly attributed to granuloma formation induced by the host cell-mediated immune response to soluble antigens secreted by parasite eggs trapped in the walls of the hepatic and intestinal vessels of infected hosts (Weber et al, 2019).…”

“…The pathophysiological impact of Schistosoma mansoni infection has been mainly attributed to granuloma formation induced by the host cell-mediated immune response to soluble antigens secreted by parasite eggs trapped in the walls of the hepatic and intestinal vessels of infected hosts (Weber et al, 2019). While the degree of Schistosoma-associated hepatic dysfunction varies and is dependent on parasite load and host background, liver fibrosis is a common finding in advanced schistosomiasis, mostly at the sites of ongoing granulomatous reactions (Ndhlovu et al, 1995). Nonetheless, a wide range of liver damage has been described in both human and animal infections.…”