Novel IMB-ELISA Assay for Rapid Diagnosis of Human Toxoplasmosis Using SAG1 Antigen

Hegazy, Shereen; Farid, Alyaa; Rabae, Ibrahim; Elamir, Azza

doi:10.7883/yoken.jjid.2014.444

Cited by 20 publications

(11 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sandwich ELISA elicited a sensitivity of 92%, a specificity of 92.7%, a positive predictive value (PPV) of 92%, and a negative predictive value (NPV) of 92.7%. Immunomagnetic bead-ELISA showed sensitivity (98%), specificity (96.4%), PPV (96%), and NPV (98.1%) which were higher than that of sandwich ELISA [13] .…”

Section: Discussionmentioning

confidence: 79%

See 1 more Smart Citation

Evaluation of the diagnostic performance of Nano gold beads-based ELISA for Detection of circulating Toxoplasma surface antigen grade III (SAG3) in Serum Samples of Infected cases.

El-Kholy

Shaheen

Baiuomy

et al. 2020

Journal of Recent Advances in Medicine

View full text Add to dashboard Cite

Objective: This study aimed to evaluate the diagnostic performance of Nanogold beads-based ELISA in serodiagnosis of human toxoplasmosis based on detection of circulating Toxoplasma surface antigen grade 3 (SAG3). Material and Methods: This study was conducted on 100 serum samples. They were divided into 3 groups: group (I): 50 samples with positive IgG antibodies for Toxoplasma gondii &group (II):30 serum samples harboring other parasites than T. gondii which was divided as following, ten infected with Schistosoma mansoni, ten infected with Giardia lamblia and ten samples infected with Entamoeba histolytica while group (III): 20 free samples from toxoplasmosis and other parasitic infections. Preparation of Toxoplasma surface antigen 3 (SAG3) was done. After purification of the polyclonal antibodies it fractionated into three fractions. Conjugation of one fraction with Horseradish peroxidase (HRP) was carried out then conjugation of other fraction polyclonal antibodies with gold nanoparticles. The 3rd fraction remains without conjugation. Detection of SAG3 in the sera by Sandwich ELISA and Nano sandwich ELISA was done. Results: Detection of Toxoplasma surface antigen grade 3 in the serum samples by Nano-sandwich ELISA was higher than sandwich ELISA regarding specificity (96% vs. 84%); PPV (95.7% vs. 85.2%) and diagnostic accuracy (93% vs.88%). Sandwich ELISA was higher than Nano-sandwich ELISA regarding sensitivity (92% vs.90%) and NPV (91.3 %vs.90.6 %). Conclusion: The use of gold nanoparticles showed the potential advantage of improving the diagnostic methods of toxoplasmosis based on detection of circulating Toxoplasma surface antigen grade 3 (SAG3). The Nano diagnostic assay significantly increased specificity, PPV and diagnostic accuracy. We applied this new technique for the first trial for diagnosis of human toxoplasmosis.

show abstract

Section: Discussionmentioning

confidence: 79%

“…An immunomagnetic bead-ELISA technique utilizing T. gondii IgG polyclonal antibodies coated with magnetic NPs to capture circulating surface antigen 1 gave better results than sandwich ELISA for diagnosis of human toxoplasmosis using SAG1 antigen [13] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the diagnostic performance of Nano gold beads-based ELISA for Detection of circulating Toxoplasma surface antigen grade III (SAG3) in Serum Samples of Infected cases.

El-Kholy

Shaheen

Baiuomy

et al. 2020

Journal of Recent Advances in Medicine

View full text Add to dashboard Cite

show abstract

“…Nanotechnology may improve sensitivity, selectivity, speed, cost, and convenience of diagnostic tests (Hegazy et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Nanotechnology may improve the sensitivity, specificity, speed, cost and convenience of diagnostic tests. Furthermore, nanotechnology has also opened up the possibility of other screening strategies (Hegazy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Nano-Graphene Based Sandwich and Dot-Elisa as Promising Techniques for Diagnosis of Human Intestinal Giardiasis

El-Lessy

Zekry

Hasan

et al. 2019

Journal of the Egyptian Society of Parasitology

View full text Add to dashboard Cite

Giardia intestinalis nowadays is recognized as the most common parasitological cause of diarrhea, with 280 million infections per year. Microscopic examination of faecal samples has the advantage of low cost and the ability to simultaneously identify other parasitic infections. However, analysis of single stool sample and the skill of the microscopist can affect the accuracy of detection.As an attempt to improve the sensitivity of laboratory diagnosis of giardiasis, the present study aimed to evaluate the diagnostic performance of different parasitological techniques (Mini Parasep, MIFC and direct smear) and to evaluate a novel antigen capture immunoassays based on IgG polyclonal antibody conjugated with nanoparticles (Nano graphene based Sandwich ELISA and Dot-ELISA) for detection of Giardia antigen in stool samples. A total of 96 human stool samples were collected and classified into three groups according to stool examination results, (GI), 61 Giardia infected patients, (GII), 20 samples collected from patients infected with other parasites and (G III), 15 healthy individuals (negative control).In the current study, Giardia antigen detection was carried out by several steps including preparation of Giardia antigen, production, purification and labeling of rabbit anti-Giardia IgG polyclonal antibodies.

show abstract

“…With the evolutionary advances in nanotechnology, IMB based-ELISA has been introduced in order to reduce the total time for analyses and improve the sensitivity [23] . Several publications utilized IMB based-ELISA to isolate nucleic acids, proteins and cells to be used in diagnosis of many parasitic infections as schistosomiasis mansoni [24] , schistosomiasis japonicum [25] , toxoplasmosis [26] , and neurocysticercosis [27] . In 2015, Hegazy et al [26] found that IMB based-ELISA has higher sensitivity (98%), specificity (96.4%), positive predictive value (96%) and negative predictive value (98.1%) than conventional ELISA when evaluated in the diagnosis of human toxoplasmosis using surface antigen (SAG1).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a developed IMB based-ELISA in diagnosis of urinary schistosomiasis in areas at risk in Upper Egypt

Mahmoud

Saad

Bayoumi

et al. 2021

Parasitologists United Journal

View full text Add to dashboard Cite

Background: Nanotechnology enables researchers to boost accuracy of the existing diagnostic techniques. Immunomagnetic beads (IMB) based-ELISA was utilized to diagnose several parasitic diseases; schistosomiasis mansoni and japonicum, toxoplasmosis and neurocysticercosis. Objectives: The present work aims to develop and evaluate a novel nano-diagnostic assay using gold nanoparticles (AuNPs) in ELISA (IMB based-ELISA) for the diagnosis of urinary schistosomiasis. Subjects and Methods: IMB based-ELISA assay was developed by preparation of polycolonal antibodies (pAbs) against Schistosoma soluble egg antigen (SEA). The developed novel assay was evaluated in urine samples of 290 schoolchildren collected from primary and preparatory schools in four villages in Beni-Suef governorate, Egypt. Urine samples were screened by chemical reagent strips (Combi 10) and examined by urine microscopy (UM). The conventional ELISA technique was used to evaluate the efficacy of IMB based-ELISA using UM as the gold standard method for diagnosis of urinary schistosomiasis. Results: The novel IMB based-ELISA assay succeeded to diagnose 50 out of 290 schoolchildren (17.2%). In comparison with other methods, results showed that 39/290 (13.4%) were positive by UM and 53/290 (18.3%) by conventional ELISA. A sensitivity, specificity and diagnostic accuracy of the evaluated ELISA assay using UM as the gold standard method were 94.87%, 95.22% and 94.48% respectively. It was observed that Combi-10 gave sensitivity and specificity of 35.9% and 94.9% respectively for microhaematuria and proteinuria. Conclusion: IMB-ELISA based on AuNPs provides a more rapid as well as sensitive detection of SEA in urine samples of patients with active schistosomiasis. Its high sensitivity and specificity ensure its application in field studies. Additionally, urinary schistosomiasis proved highly prevalent in schoolchildren living in Beni-Suef villages.

show abstract

Novel IMB-ELISA Assay for Rapid Diagnosis of Human Toxoplasmosis Using SAG1 Antigen

Cited by 20 publications

References 65 publications

Evaluation of the diagnostic performance of Nano gold beads-based ELISA for Detection of circulating Toxoplasma surface antigen grade III (SAG3) in Serum Samples of Infected cases.

Evaluation of the diagnostic performance of Nano gold beads-based ELISA for Detection of circulating Toxoplasma surface antigen grade III (SAG3) in Serum Samples of Infected cases.

Evaluation of Nano-Graphene Based Sandwich and Dot-Elisa as Promising Techniques for Diagnosis of Human Intestinal Giardiasis

Evaluation of a developed IMB based-ELISA in diagnosis of urinary schistosomiasis in areas at risk in Upper Egypt

Contact Info

Product

Resources

About