Optimization of the growth of and α-amylase production by Bacillus subtilis IP 5832 in shake flask and laboratory fermenter batch cultures

Božić, Nataša; Ruiz, Jordi; López-Santı́n, Josep; Vujčić, Zoran

doi:10.2298/jsc101010098b

Cited by 17 publications

(10 citation statements)

References 16 publications

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“…Yeast extract had been reported to play an important role in enzyme production due to the presence of essential elements, coenzymes, nitrogenous constituents and growth factors [15]. However, our reports were against the observations of Bozic et al [18] who demonstrated casein to be the best nitrogen source for amylase production from Bacillus sp.…”

Section: Discussioncontrasting

confidence: 76%

Optimization of Thermo-Alkali Stable Amylase Production and Biomass Yield from Bacillus sp. Under Submerged Cultivation

Khusro¹,

Barathikannan²,

Aarti³

et al. 2017

Fermentation

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Abstract:The present context was investigated to optimize amylase production and cell biomass of poultry-associated Bacillus sp. using a conventional as well as statistical approach. Box-Behnken design (BBD) matrix at N = 29 was employed to optimize four independent variables, selected from one factor at a time (OFAT) technique, for maximum amylase production and biomass yield. The relative activity of crude amylase obtained from the isolate showed stability at high temperature (60 • C) and alkaline condition (pH 9) up to 4 h of incubation, thereby indicating its alkali-tolerant and thermo-stable property. The BBD resulted in enhanced amylase activity of 145.32 U/mL when the basal medium was slightly acidic (pH 6) and kept at a temperature of 35 • C with the shaking speed of 130 rpm, in addition to being incubated for 24 h. The selected factors, when employed with this statistical optimization approach, showed 1.5-fold and 2-fold enhancements in the amylase production and biomass yield respectively compared to the OFAT method. Analysis of variance (ANOVA) revealed high coefficient of determination (R 2 ) of 0.96 to 0.99 for both the responses at significant level (p < 0.05). Three-dimensional response and 2D contour plot of the quadratic model showed interdependent interaction between the effective variables. Long-term thermo-alkali stability of amylase obtained from Bacillus sp. suggested not only its wide applications in pharmaceutical, food and biotechnological industries, but also suggested a potent replacement of existing amylases on the market.

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Section: Discussioncontrasting

confidence: 76%

Optimization of Thermo-Alkali Stable Amylase Production and Biomass Yield from Bacillus sp. Under Submerged Cultivation

Khusro¹,

Barathikannan²,

Aarti³

et al. 2017

Fermentation

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“…Yeast extract was found to be the best nitrogen source for all the isolates in order to achieve maximum production of amylase. The present investigation supports the finding of Ravindar and Elangovan (2013) and Dash et al (2015) but do not correlate with the finding of Bozic et al (2011), who demonstrated tryptone as a potential nitrogen source for enzyme production. Yeast extract has been reported to play an important role in enzyme production due to the presence of essential elements and growth factors (Porsuk et al, 2013).…”

Section: Bacillus Mojavensis Strain Mn43supporting

confidence: 55%

Molecular Identification of Newly Isolated Bacillus Strains from Poultry Farm and Optimization of Process Parameters for Enhanced Production of Extracellular Amylase Using OFAT Method

Khusro¹,

Aarti²

2015

Research J. of Microbiology

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Bacillus sp. has been used as attractive and efficient industrial organisms in the last few decades due to their rapid growth rates and their potential to secrete extracellular enzymes into the medium. Amylase obtained from bacteria has gained immense interest due to its biotechnological potential in various industries. The aim of this experimental context was to isolate novel strains of Bacillus sp. from poultry farm, screening and optimization of different parameters for the enhanced production of extracellular amylase using traditional OFAT method (One Factor At a Time). Five hyperamylase producing Bacillus strains were identified after preliminary screening of poultry feces soil sample. The strains were showing maximum amylase production at pH ranging from 6-9. On the other hand strains were showing more enzyme activity at 35 and 45°C. The agitation speed of 130 and 150 rpm was found to be suitable for maximum enzyme production by Bacillus sp. The amylase activity of each isolate was reduced with increase in incubation time from 24-96 h. Starch and yeast extract were found to be the suitable carbon and nitrogen source in order to enhance the enzyme production. The enzyme obtained from the isolates was stable upto 60°C and pH 9.0 for 4 h of incubation. Percentage GC-content of 16S rRNA sequences showed a significant effect on amylase production at high temperature. The present study clearly indicates that amylase production was 2 fold higher when optimized conditions were used. The crude amylase obtained from the isolates showed stability at high temperature and pH. As to the best of our knowledge this present investigation makes a novel contribution to research on amylase production from new Bacillus strains of poultry farm by demonstrating the isolation, molecular characterization and enhanced amylase activity at optimal conditions using OFAT method.

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“…In this study, the maximum amylase production was observed 0.2% starch as sole source of carbon when compared to other carbon sources tested for B. licheniformis KSU-6. These results are supported with the previous reports stated that the amylase production was increased when starch used as carbon sources (Lachmund and Ruttkowski, 1990;Lin et al, 1998;Gupta et al, 2003;Saxena et al, 2007;Bozic et al, 2011). The nitrogen sources have influenced the extracellular amylase enzyme production and also supplement with specific nitrogen source on enzyme production differs from organism to organism (Hillier et al, 1996).…”

Section: F)supporting

confidence: 90%

Production, Optimization and Partial Characterization of Thermostable and Alkaline Amylase from Bacillus licheniformis KSU-6

Nithya¹,

Muthukumar²,

Dhanasekaran³

et al. 2016

IJAB

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Total of 56 bacterial isolates were isolated from five palm tree cultivated soil samples and screened their amylase production. In the preliminary screening, twenty four isolates exhibited amylase producing ability. Among these 24 isolates, an isolate KSU-6 showed maximum amylase enzyme production (37.52 U mL -1 ), and the isolate was identified as Bacillus licheniformis KSU-6. The maximum amylase production was optimized, and the conditions were pH 10.0, temperature 50 o C, incubation period 44 h, inoculum concentration 2% (v/v), starch 0.2% (w/v) as carbon source, peptone 0.5% (w/v) along with malt extract 0.3% (w/v) as nitrogen sources for B. licheniformis KSU-6. The partially purified amylase stabled upto pH 12, temperature 80 o C, 1-2 M salt concentration and 10 mM of different detergents. In this study conclude that the soil bacterium Bacillus licheniformis KSU-6 produced thermostable and alkaline amylase, which could be useful for industrial liquefaction and detergent industries.

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Optimization of the growth of and α-amylase production by Bacillus subtilis IP 5832 in shake flask and laboratory fermenter batch cultures

Cited by 17 publications

References 16 publications

Optimization of Thermo-Alkali Stable Amylase Production and Biomass Yield from Bacillus sp. Under Submerged Cultivation

Optimization of Thermo-Alkali Stable Amylase Production and Biomass Yield from Bacillus sp. Under Submerged Cultivation

Molecular Identification of Newly Isolated Bacillus Strains from Poultry Farm and Optimization of Process Parameters for Enhanced Production of Extracellular Amylase Using OFAT Method

Production, Optimization and Partial Characterization of Thermostable and Alkaline Amylase from Bacillus licheniformis KSU-6

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