Optimization of Thermo-Alkali Stable Amylase Production and Biomass Yield from Bacillus sp. Under Submerged Cultivation

Abstract

Abstract:The present context was investigated to optimize amylase production and cell biomass of poultry-associated Bacillus sp. using a conventional as well as statistical approach. Box-Behnken design (BBD) matrix at N = 29 was employed to optimize four independent variables, selected from one factor at a time (OFAT) technique, for maximum amylase production and biomass yield. The relative activity of crude amylase obtained from the isolate showed stability at high temperature (60 • C) and alkaline condition (pH 9) up to 4 h of incubation, thereby indicating its alkali-tolerant and thermo-stable property. The BBD resulted in enhanced amylase activity of 145.32 U/mL when the basal medium was slightly acidic (pH 6) and kept at a temperature of 35 • C with the shaking speed of 130 rpm, in addition to being incubated for 24 h. The selected factors, when employed with this statistical optimization approach, showed 1.5-fold and 2-fold enhancements in the amylase production and biomass yield respectively compared to the OFAT method. Analysis of variance (ANOVA) revealed high coefficient of determination (R 2 ) of 0.96 to 0.99 for both the responses at significant level (p < 0.05). Three-dimensional response and 2D contour plot of the quadratic model showed interdependent interaction between the effective variables. Long-term thermo-alkali stability of amylase obtained from Bacillus sp. suggested not only its wide applications in pharmaceutical, food and biotechnological industries, but also suggested a potent replacement of existing amylases on the market.

Optimization of Bacteria Amylase Activity from Bacillus licheniformis Strain SEM11

“…The thermostable and alkaline amylases are also produced by Bacillus cereus with a temperature of 65 °C and pH 8.0 (Annamalai et al, 2011). In contrast to SS1, SS2, and SS3 isolates having an optimum pH of amylase activity at pH 7.0 (Kumar et al, 2012). pH affects the velocity of enzyme activity in catalyzing a reaction.…”

“…Bacteria amylases have a broad application in industries due to their stability, high enzyme activity at various parameters and cost-effective production (Khusro et al, 2017). The production of enzymes by microorganisms as well as the enzyme yield depends on the nutritional factors especially carbon and nitrogen sources (Kumar et al, 2012). The purpose of this study was to increase bacteria amylase activity of Bacillus licheniformis strain SEM11 through optimization of bacteria amylase activity and production medium.…”

“…Therefore it is necessary to increase bacteria amylase activity through optimization of bacteria amylase activity and production medium, such as optimization of substrates, substrates concentration, inoculum concentration, agitation speed, and nutritional factors, especially carbon and nitrogen sources. Bacteria amylases have a broad application in industries due to their stability, high enzyme activity at various parameters and cost-effective production (Khusro et al, 2017). The production of enzymes by microorganisms as well as the enzyme yield depends on the nutritional factors especially carbon and nitrogen sources (Kumar et al, 2012).…”

“…In contrast to Halder et al, (2014), Casein is the best source of nitrogen in the production of bacteria amylase. Nitrogen source served as a secondary energy source for growth and enzyme secretion (Kumar et al, 2012).…”

Isolation, Screening and Determination of Α-Amylase Activity From Marine Streptomyces Species

“…Aquatic actinomycetes were exhibited amylase activity 62.97 U/ml [41]. Box-Behnken design method highly improved amylase activity 145.32 U/ml (pH 6, temp 35 ° C) [42]. Aspergillus fumigatus NTCC1222 showed highest amylase activity (164.1 U/ml) was studied [43].…”