Optimization of the extraction process of polyphenols from Allium cepa using response surface methodology and assessment of its antioxidant and lipid-lowering action

Fan, Kongming; Yang, Xiaoqian; Zhou, Chunyang; Mei, Xue; Yang, Xin; Fu, Qiaofeng; Li, Yan; Lin, Yong; Yang, Chunyan

doi:10.1007/s11694-021-01239-4

Cited by 6 publications

(7 citation statements)

References 40 publications

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“…The correlation between independent variables and response values has been shown Y 1 = 2.8700 + 0.0463 A − 0.1487 B − 0.0125 C − 0.0025 italicAB − 0.0000 italicAC − 0.0000 italicBC − 0.1218 A 2 − 0.1718 B 2 − 0.0592 C 2 Y 2 = 4.4400 + 0.0587 A − 0.2313 B − 0.0175 C − 0.0500 italicAB + 0.0025 italicAC − 0.0075 italicBC − 0.0890 A 2 − 0.2140 B 2 − 0.0065 C 2 Y 3 = 2.4100 + 0.0312 A − 0.1225 B − 0.0187 C − 0.0050 italicAB + 0.0025 italicAC + 0.0000 italicBC − 0.1148 A 2 − 0.1522 B 2 − 0.0348 C 2 where Y 1 (μg/mL), Y 2 (μg/mL), and Y 3 (μg/mL) represent the concentrations of p-hydroxybenzoic acid, quercetin, and paclitaxel, respectively, and A (%), B (μL/min), and C (cm) represent the volume fraction of methanol, flow rate, and length of membrane tube, respectively. The regression model was analyzed, and it was found that the P values of the “model” items in the three groups of experiments were all less than 0.005, while the P values of the “lack of deviation” were all more than 0.05, which means that the regression effect is significant . The R 2 of the three groups of...…”

Section: Resultsmentioning

confidence: 96%

“…The correlation between independent variables and response values has been shown where Y 1 (μg/mL), Y 2 (μg/mL), and Y 3 (μg/mL) represent the concentrations of p-hydroxybenzoic acid, quercetin, and paclitaxel, respectively, and A (%), B (μL/min), and C (cm) represent the volume fraction of methanol, flow rate, and length of membrane tube, respectively. The regression model was analyzed, and it was found that the P values of the “model” items in the three groups of experiments were all less than 0.005, while the P values of the “lack of deviation” were all more than 0.05, which means that the regression effect is significant . The R 2 of the three groups of models are 0.9253, 0.9781, and 0.9910, respectively, while the differences between the R 2 predicted by the three models and the adjusted R 2 are lower than 0.2, showing good applicability.…”

Section: Resultsmentioning

confidence: 97%

“…The regression model was analyzed, and it was found that the P values of the "model" items in the three groups of experiments were all less than 0.005, while the P values of the "lack of deviation" were all more than 0.05, which means that the regression effect is significant. 35 The R 2 of the three groups of models are 0.9253, 0.9781, and 0.9910, respectively, while the differences between the R 2 predicted by the three models and the adjusted R 2 are lower than 0.2, showing good applicability. By analyzing the 3D model, we can better analyze the content changes of the three allelochemicals.…”

Section: Box−behnken Design Experimental (Bbd) and Response Surface A...mentioning

confidence: 89%

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Quick and In Situ Detection of Different Polar Allelochemicals in Taxus Soil by Microdialysis Combined with UPLC-MS/MS

Qiao

Nie

et al. 2022

J. Agric. Food Chem.

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The action of allelopathy need that allelochemicals exist in the soil and reach a certain concentration. Also, the detection of allelochemicals in the soil is one of the most important research topics in the process of exploring allelopathy. To solve the problem of the simultaneous detection of allelochemicals with low concentrations and different polarities, a novel strategy for the quick detection of the allelochemicals in Taxus soil by microdialysis combined with UPLC-MS/MS on the basis of in situ detection without destroying the original structure of soil was developed for the first time in the work. The dialysis conditions were optimized by the Box–Behnken design (BBD): 70% methanol, 3 μL/min flow rate, and 3 cm long membrane tube. A reliable UPLC-MS/MS program was systematically optimized for the simultaneous detection of nine allelochemicals with different polarities. The results proved the differences in the contents and distributions of nine allelochemicals in three different Taxus soils.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 97%

Section: Box−behnken Design Experimental (Bbd) and Response Surface A...mentioning

confidence: 89%

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Quick and In Situ Detection of Different Polar Allelochemicals in Taxus Soil by Microdialysis Combined with UPLC-MS/MS

Qiao

Nie

et al. 2022

J. Agric. Food Chem.

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“…Preparation of the Solutions ACE, ACA, AC30%, AC50%, AC70%, and AC95% were precisely weighed, and 200 µL of DMSO plus 3.8 mL of purified water were added to obtain the solutions, i.e., 22.125 mg/mL of ACE, 32.95 mg/mL of ACA, 15.225 mg/mL of AC30%, 13.975 mg/mL of AC50%, 3.225 mg/mL of AC70%, and 5.075 mg/mL of AC95%. The preparation of simvastatin was completed on the basis of the literature [23].…”

Section: Preparation Of Abts +• and Dpph • Solutionsmentioning

confidence: 99%

“…The cell viability experiment was conducted according to the literature [23]. Briefly, 100 µL of the cells growing in logarithmic phase were inoculated in 96-well plates.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Bio-Assay-Guided Isolation of Fractions and Constituents with Antioxidant and Lipid-lowering Activity from Allium cepa

Fan

et al. 2023

Antioxidants

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Active fractions and constituents with antioxidant and lipid-lowering activities were investigated using bio-assay-guided isolation and identification. The data showed that the antioxidant fraction of A. cepa was AC50%, the main constituents of which were quercetin and isoquercitrin, by way of both ultra-high performance liquid chromatography–mass spectrometry (UPLC-MS) and bio-assay-guided purification and elucidation. Similarly, the lipid-lowering active fraction of A. cepa was AC30% with the main constituents of 3,4-dihydroxybenzoic acid and quercetin 3,4′-O-diglucoside. Also, bio-assay-guided isolation led to the isolation and identification of five known compounds with a purity of more than 98%, and quercetin was both the best free radical scavenger and lipid-lowering constituent. Moreover, the mechanism of the lipid-lowering effect of AC30% might be its reduction in mRNA expression levels of sterol regulatory element binding protein 2 (SREBP2) and FAS gene in lipid synthesis. Otherwise, reducing the mRNA expression level of lipid synthesis genes, including SREBP1, SREBP2, fatty acid synthetase (FASN), β-Hydroxy β-methylglutaryl-CoA (HMGCR), stearoyl CoA desaturase 1 (SCD1), and increasing the mRNA expression level of lipid decomposition gene, such as carnitine palmitoyl transferease-1 (CPT1), might be involved in the lipid-lowering activity of quercetin. This study suggested that Allium cepa might be used to prevent and treat oxidative stress and dislipidemia-related disorders, including NAFLD.

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Comparing DES-mediated ultrasound- and heat-assisted extraction of bioactive metabolites from Saussurea lappa and optimization by RSM and validation studies

Ali,

Ahmed

2023

Green Analytical Chemistry

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Optimization of the extraction process of polyphenols from Allium cepa using response surface methodology and assessment of its antioxidant and lipid-lowering action

Cited by 6 publications

References 40 publications

Quick and In Situ Detection of Different Polar Allelochemicals in Taxus Soil by Microdialysis Combined with UPLC-MS/MS

Quick and In Situ Detection of Different Polar Allelochemicals in Taxus Soil by Microdialysis Combined with UPLC-MS/MS

Bio-Assay-Guided Isolation of Fractions and Constituents with Antioxidant and Lipid-lowering Activity from Allium cepa

Comparing DES-mediated ultrasound- and heat-assisted extraction of bioactive metabolites from Saussurea lappa and optimization by RSM and validation studies

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