Optimization of the extraction of phytochemicals from black mulberry (Morus nigra L.) leaves

Nastić, Nataša; Borrás-Linares, Isabel; Lozano-Sánchez, Jesús; Švarc‐Gajić, Jaroslava; Segura‐Carretero, Antonio

doi:10.1016/j.jiec.2018.07.055

Cited by 34 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This compound was only found in Valenciana Temprana MAE, while the second one (peak 36), which provided a m / z at 221.1179 and was assigned to hexyl salicylate, was presented in all genotypes. Finally, peak 52, which was detected in all MAEs, was tentatively identified as gingerol [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Leyva-Jiménez¹,

Ruiz-Malagón

Molina-Tijeras

et al. 2020

Antioxidants

Self Cite

View full text Add to dashboard Cite

Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH· radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Leyva-Jiménez¹,

Ruiz-Malagón

Molina-Tijeras

et al. 2020

Antioxidants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Compounds eluting as Peaks 18 and 20 showed a mass of 287.2228 with a predicted molecular formula of C 16 [38]. Moreover, two monounsaturated and polyunsaturated fatty acids (Peaks 43 and 44) were suggested as palmitoleic acid and linoleic acid, based on the high-resolution masses (m/z 253.2173 and 279.233, respectively) and predicted molecular formulas [38].…”

Section: Fatty Acids and Derivativesmentioning

confidence: 98%

“…Compounds eluted as peaks 17,19,22, and 23 with a [M-H] − ion at m/z 313.2384 were identified as belonging to dihydroxy-octadecenoic acid isomers by a comparison of their fragmentation patterns and bibliographic information [34,35]. Compounds eluting as Peaks 18 and 20 showed a mass of 287.2228 with a predicted molecular formula of C 16 [38]. Moreover, two monounsaturated and polyunsaturated fatty acids (Peaks 43 and 44) were suggested as palmitoleic acid and linoleic acid, based on the high-resolution masses (m/z 253.2173 and 279.233, respectively) and predicted molecular formulas [38].…”

Section: Fatty Acids and Derivativesmentioning

confidence: 99%

“…To define the phytochemical profile of comfrey root extracts, HPLC-ESI-QTOF-MS analysis was applied. Separation of the compounds was performed using an Agilent 1200 Series Rapid Resolution LC system (Agilent Technologies, Palo Alto, CA, USA) (Agilent Technologies, Palo Alto, CA, USA) of the Series Rapid Resolution coupled to an electro-spray quadrupole time-of-flight mass spectrometer, previously described by Nastić et al [38]. The mobile phase was 0.1% formic acid in water as eluent A and acetonitrile as eluent B.…”

Section: Hplc-esi-qtof-ms Analysismentioning

confidence: 99%

See 1 more Smart Citation

Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques

Nastić

Borrás-Linares²,

Lozano-Sánchez

et al. 2020

Molecules

Self Cite

View full text Add to dashboard Cite

In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of S. officinale root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more efficient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 °C. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility.

show abstract

“…Sometimes, HPLC is coupled with other techniques such as gas chromatography (GC), and modied or optimized to obtain chemical ngerprints of plants. [80][81][82][83][84][85][86] Chemical ngerprint is the constituents of a plant that determine the active ingredients and identies the available impurities. 80 HPLC is one of the important techniques for alkaloid metabolite extraction and analysis.…”

Section: Processing Of Plants To Get Extracts For Pmsmentioning

confidence: 99%

A plant-mediated synthesis of nanostructured hydroxyapatite for biomedical applications: a review

2020

View full text Add to dashboard Cite

show abstract

Optimization of the extraction of phytochemicals from black mulberry (Morus nigra L.) leaves

Cited by 34 publications

References 31 publications

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques

A plant-mediated synthesis of nanostructured hydroxyapatite for biomedical applications: a review

Contact Info

Product

Resources

About