Optimization of Surface Sterilization Process of Selected Dye-Yielding Plants for Isolation of Bacterial Endophytes

Khanam, Bushra; Chandra, Ramesh

doi:10.1007/978-981-10-5538-6_7

Cited by 10 publications

(8 citation statements)

References 6 publications

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“…It has been proven that two-phase disinfection method is beneficial for certain plant species. Ethanol usually exerts the influence in combination of sodium hypochlorite (Khanam and Chandra, 2017). In this study, it was found that sodium hypochlorite alone brought about reduction of bacterial infection, compared with ethanol alone.…”

Section: Discussionmentioning

confidence: 71%

Inhibition of browning problem during the callogenesis of Spartium junceum L.

Taghizadeh

Dastjerdi

2021

Ornam. Hortic.

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During different phases of in vitro culture, plant tissues may be exposed to some stresses that never encounter in their natural habitats. The most significant stresses which interfere with in vitro culture are pathogenic contamination and browning disorder. Since browning sign is occurred during all phases of in vitro culture of Spartium junceum L., the present study was done preventing explants from browning during disinfection and callogenesis phases using exposure time of sterilants (ethanol 0, 30, 60 s and home bleach 0, 10, 15 min), antioxidant compounds (PVP 0.5%, Activated charcoal 0.1%, Curcumin 0.1%), Running water (30 and 60 min) plant growth regulators (2,4-D 0, 0.5, 1 and 2 mg L-1 and BA 0, 0.1 and 0.2 mg L-1), and by changing light/dark conditions was designed. The results showed that ethanol 70% (30 s) in combination with home bleach 20% (10 min) had the best effect in control contaminations and browning sign in nodal explants of S. junceum. The application of PVP 0.5% in medium was the best treatment to control of browning nodal explants in callus induction phase. The highest callus formation and the lowest explant browning were obtained on the medium supplemented with 0.5 mg L-1 2,4-D under the darkness condition. According to the results of this study, how disinfection methods, culture medium compositions and light conditions were effective on the browning and callogenesis of Spartium junceum L.

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Section: Discussionmentioning

confidence: 71%

Inhibition of browning problem during the callogenesis of Spartium junceum L.

Taghizadeh

Dastjerdi

2021

Ornam. Hortic.

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“…In addition, pretreatment with surfactants like Triton X-100, Tween 80, Tween 20, and Teepol are also used to enhance efficiency [27][28][29]. Despite gigantic progress in endophyte research, detailed reviews on different aspects of surface sterilization protocols were meager, especially with respect to the type of surface sterilizing agents, their concentration, and exposure time [24,30,31]. Thus, an exhaustive compilation of surface sterilants, sterilization methods, and other aspects on endophyte isolation are thoroughly discussed in this review.…”

Section: Need For Isolation Of Endophytic Microbiotamentioning

confidence: 99%

“…This becomes even more crucial where the objective of the experiment is to determine the overall endophyte diversity. In that case, the consideration should be to minimize the killing of endophytic bacteria during surface sterilization [30,66]. On the other hand, stringent surface sterilization could be done to isolate a true endophyte with a specific function.…”

Section: Efficiency Of Surface Sterilantsmentioning

confidence: 99%

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Surface sterilization for isolation of endophytes: Ensuring what (not) to grow

et al. 2022

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Endophytic microbiota opens a magnificent arena of metabolites that served as a potential source of medicines for treating a variety of ailments and having prospective uses in agriculture, food, cosmetics, and many more. There are umpteen reports of endophytes improving the growth and tolerance of plants. In addition, endophytes from lifesaving drug-producing plants such as Taxus, Nothapodytes, Catharanthus, and so forth have the ability to produce host mimicking compounds. To harness these benefits, it is imperative to isolate the true endophytes, not the surface microflora. The foremost step in endophyte

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“…In Method I, samples were surface sterilized with 70% ethanol for 8 min (Tian et al, 2007). In Method II, samples were immersed in 70% ethanol for 2 min, then washed with 1% sodium hypochlorite for 3 min, and finally rinsed with 70% ethanol for 1 min (Khanam and Chandra, 2017). In Method III, samples were surface sterilized with 0.1% mercuric chloride for 2 min, 70% ethanol for 1 min, and 2% sodium hypochlorite for 5 min (Baldan et al, 2014).…”

Section: Surface Sterilizationmentioning

confidence: 99%

Diversity, Antimicrobial Activity, and Antibiotic Susceptibility Pattern of Endophytic Bacteria Sourced From Cordia dichotoma L

Sharma

Mallubhotla

2022

Front. Microbiol.

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Endophytic bacteria isolated from medicinal plants are crucial for the production of antimicrobial agents since they are capable of possessing bioactive compounds with diverse structures and activities. Cordia dichotoma, a plant of medicinal importance native to the Jammu region of India, was selected for the isolation and characterization of culturable endophytic bacteria and evaluation of their antimicrobial activities. Standardized surface sterilization methods were employed to isolate thirty-three phenotypically distinguishable endophytic bacteria from the root, stem, and leaf parts of the plant. Shannon Wiener diversity index clearly divulged diverse endophytes in roots (0.85), stem (0.61), and leaf (0.54) tissues. Physio-biochemical features of the isolates differentiated the distinct variations in their carbohydrate utilization profile and NaCl tolerance. The endophytes produced an array of enzymes, namely, catalase, oxidase, amylase, cellulase, nitrate reductase, and lipase. The bacterial isolates belong to the genera Bacillus, Pseudomonas, Paenibacillus, Acidomonas, Streptococcus, Ralstonia, Micrococcus, Staphylococcus, and Alcalignes predominantly. However, the antibiotic susceptibility pattern indicated that the isolates were mostly sensitive to erythromycin and streptomycin, while they were resistant to rifampicin, amoxicillin, and bacitracin. Interestingly, majority of the bacterial endophytes of C. dichotoma showed antimicrobial activity against Bacillus subtilis followed by Klebsiella pneumoniae. The 16S rRNA sequence of Bacillus thuringiensis has been deposited in the NCBI GenBank database under accession number OM320575. The major compounds of the crude extract derived from endophytic B. thuringiensis OM320575, according to the metabolic profile examination by GC-MS, are dibutyl phthalate, eicosane, tetrapentacontane, heneicosane, and hexadecane, which possessed antibacterial activities. In conclusion, results indicated the potential of C. dichotoma to host a plethora of bacterial endophytes that produce therapeutic bioactive metabolites.

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Optimization of Surface Sterilization Process of Selected Dye-Yielding Plants for Isolation of Bacterial Endophytes

Cited by 10 publications

References 6 publications

Inhibition of browning problem during the callogenesis of Spartium junceum L.

Inhibition of browning problem during the callogenesis of Spartium junceum L.

Surface sterilization for isolation of endophytes: Ensuring what (not) to grow

Diversity, Antimicrobial Activity, and Antibiotic Susceptibility Pattern of Endophytic Bacteria Sourced From Cordia dichotoma L

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