Optimization of substrate concentration of dilute acid hydrolyzate of lignocellulosic biomass in batch hydrogen production

Gonzales, Ralph Rolly; Sivagurunathan, Periyasamy; Anburajan, Parthiban; Kim, Jun Seok

doi:10.1016/j.ibiod.2016.04.016

Cited by 60 publications

(16 citation statements)

References 22 publications

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“…Each step of biomass treatment affects its consecutive processing. The harsh pH conditions of chemical hydrolysis, for example, require further pH adjustment before the enzymatic hydrolysis and may also result in the formation of undesired products [ 20 , 54 , 55 , 56 , 57 ]. Thus, the harsh conditions of acid pre-treatment may lead to the degradation of monomeric sugars into by-products such as 5-hydroxymethylfurfural or furfural, which may inhibit the fermentative hydrogen production [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

et al. 2018

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The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification. The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal. Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions. The research was carried out using the Box-Behnken design. Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated. Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H2/g energy poplar (0.6 mol H2/mol TRS).

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Section: Introductionmentioning

confidence: 99%

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

et al. 2018

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“…In addition, furfural contained in media with anaerobic conditions will be reduced to furfuryl alcohol. These two compounds would undoubtedly inhibit the metabolism of micro-organisms for hydrogen production [5] . From the study conducted by Gonzales et al [8] , using sulfuric acid of 5% (v/v) at 121 for 90 minutes, the resulting furfural concentration reached 12,47 g/L.…”

Section: Degradation Productsmentioning

confidence: 99%

“…Some physicochemical methods such as pretreatment using alkaline solvents such as sodium hydroxide and using acid solvents such as sulfuric acid, acetic acid have been widely applied. Physicochemical pretreatment using diluted sulfuric acid is the most commonly employed method because it is simple, relatively cheap, and produces high sugar yields [5,6] . The strength of the cellulose and hemicellulose chain has certain stability in acidic and thermal conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of Dilute Acid Pretreatment of Vegetable Waste on Sugar Production and Inhibitor Formation

Rahman¹,

Aparamarta²,

Widjaja³

2020

JTS

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Vegetable waste is an organic waste with high cellulose and hemicellulose and low lignin content. The cellulose and hemicellulose chains can be broken down by pretreatment using sulfuric acid to obtain reducing sugar. To avoid the formation of degradation products that have the potential as inhibitor compounds, the temperature of the pretreatment operation was carried out at 121 and 125 for 60 minutes with sulfuric acid concentrations varying from 0.5% to 1.5% (v/v). The solid and liquid ratio (S/L) was 5% (w/v). From the experiments, the highest total reducing sugars of 7.068 g/L was obtained by pretreatment conditions at 121 for 60 minutes with a sulfuric acid concentration of 1% (v/v). Meanwhile, the lowest total reducing sugar of 2.764 g/L was produced during the pretreatment operating conditions at 125 for 60 minutes with a sulfuric acid concentration of 1% (v/v). Under the present experimental condition, it was found that only a low level of degradation product was formed, which ensures excellent performance of bacterial growth in the subsequent fermentation process.

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“…Therefore, alternatives such as hydrogen ethanol and butanol from biomass are used to resolve the petroleum-based energy crisis. Bio-hydrogen is a renewable energy that provides high energy value and does not cause greenhouse gas emissions during its combustion [3]. Bio-butanol as liquid fuel has high potential comparable to ethanol and gasoline and 96 octane number similar to gasoline.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production

et al. 2019

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This paper reports on the optimum conditions for simultaneous hydrogen and butyric acid production from microalgae (Chlorella sp.) using enriched anaerobic mixed cultures as inoculum. The fermentation was objectively carried out under acidogenic conditions to achieve butyric acid for further ABE fermentation in solventogenesis stage. The main effects of initial pH (5 and 7), temperature (35 °C and 55 °C), and substrate concentration (40, 60, 80, and 100 g-VS/L) for hydrogen and butyric acid production were evaluated by using batch fermentation experiment. The major effects on hydrogen and butyric acid production are pH and temperature. The highest production of hydrogen and butyric acid was observed at pH 7 and temperature 35 °C. Using initial Chlorella sp. concentration of 80 g-VS/L or 100 g-VS/L at pH 7 and temperature 35 °C could produce hydrogen with an average yield of 22 mL-H2/g-VS along with high butyric acid production yield of 0.05 g/g-VS, suggesting that microalgae (Chlorella sp.) has potential to be converted directly to butyric acid by using acidogenesis under above optimum conditions.

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Optimization of substrate concentration of dilute acid hydrolyzate of lignocellulosic biomass in batch hydrogen production

Cited by 60 publications

References 22 publications

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

Effect of Dilute Acid Pretreatment of Vegetable Waste on Sugar Production and Inhibitor Formation

Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production

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