Optimization of Steam Pretreatment of SO2-Impregnated Corn Stover for Fuel Ethanol Production

Öhgren, Karin; Galbe, Mats; Zacchi, Guido

doi:10.1007/978-1-59259-991-2_89

Cited by 20 publications

(28 citation statements)

References 14 publications

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“…The xylose lost after a 30 min treatment might be caused by degradation of hemicellulose at 195°C. It has been reported that the degradation of xylose is much easier and faster than that of glucose [21][22][23][24]. Figure 2 shows the result of the sugar concentrations in the hydrolysates from the two-step pretreatment at four temperatures in the second pretreatment with hot water.…”

Section: Electrolyzed Water Pretreatmentmentioning

confidence: 99%

Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method

Wang

Feng

2012

Chin. Sci. Bull.

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Pretreatment is one of the most important unit operations for ethanol production from biomass feedstocks. In this study, corn stover was used as a feedstock to examine the effectiveness of two pretreatments: electrolyzed water pretreatment and a two-step pretreatment. Electrolyzed water was employed as a catalyst to conduct one-step pretreatment of corn stover at three temperatures (165, 180 and 195°C) and three treatment times (10, 20 and 30 min). During the two-step pretreatment process, an organic alkaline solution of 1% (w/w) NaOH in 70% (w/w) ethanol was used for lignin removal in the first step, followed by a second step using hot water. No furfural or 5-hydroxymethyl furfural was detected in the hydrolysates from both pretreatment methods when the detection limit of the HPLC was 0.2 g/L. The highest glucan conversion yields were 83% obtained at 195°C for 30 min with acidic electrolyzed water and 83% by the two-step process, where the second step of the pretreatment was at 135°C for 30 min. The hydrolyzates from the two pretreatment methods showed good performance in Saccharomyces cerevisiae fermentation tests. The two new methods may provide promising alternatives for the pretreatment of biomass for ethanol production. ethanol, biomass, pretreatment, inhibitor Citation:Wang X J, Feng H, Li Z Y. Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method.

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Section: Electrolyzed Water Pretreatmentmentioning

confidence: 99%

Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method

Wang

Feng

2012

Chin. Sci. Bull.

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“…So far, methods such as ammonia fiber explosion (AFEX), wet oxidation and liquid hot water (LHW) treatment seem to be more successful for agricultural residues (11,65,66), whereas steam pretreatment has resulted in high sugar yields for both forestry and agricultural residues. Glucose yields >90% and xylose yields >80% were obtained after enzymatic hydrolysis, both with and without the addition of an acid catalyst (43,46,54,58).…”

Section: Pretreatmentmentioning

confidence: 98%

Perspectives for the Production of Bioethanol from Lignocellulosic Materials

Petrova

Иванова

2010

Biotechnology & Biotechnological Equipment

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“…The pretreatments were performed at near optimal conditions that had previously been determined to provide maximum hemicellulose recovery while ensuring effective enzymatic hydrolysis of the cellulose component (steam pretreatment: corn stover [34], Douglas-fir [35], and poplar [36]). After pretreatment, the cellulose rich water insoluble components were washed, filtered and refrigerated for long-term storage.…”

Section: Lignocellulosic Feedstocks and Their Pretreatmentmentioning

confidence: 99%

The Development and Use of an Elisa-Based Method to Follow the Distribution of Cellulase Monocomponents During the Hydrolysis of Pretreated Corn Stover

Pribowo¹,

Hu²,

Arantes³

et al. 2015

Fuel Production From Non-Food Biomass

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Background: It is widely recognised that fast, effective hydrolysis of pretreated lignocellulosic substrates requires the synergistic action of multiple types of hydrolytic and some non-hydrolytic proteins. However, due to the complexity of the enzyme mixture, the enzymes interaction with and interference from the substrate and a lack of specific methods to follow the distribution of individual enzymes during hydrolysis, most of enzyme-substrate interaction studies have used purified enzymes and pure cellulose model substrates. As the enzymes present in a typical "cellulase mixture" need to work cooperatively to achieve effective hydrolysis, the action of one enzyme is likely to influence the behaviour of others. The action of the enzymes will be further influenced by the nature of the lignocellulosic substrate. Therefore, it would be beneficial if a method could be developed that allowed us to follow some of the individual enzymes present in a cellulase mixture during hydrolysis of more commercially realistic biomass substrates. Results: A high throughput immunoassay that could quantitatively and specifically follow individual cellulase enzymes during hydrolysis was developed. Using monoclonal and polyclonal antibodies (MAb and PAb, respectively), a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed to specifically quantify cellulase enzymes from Trichoderma reesei: cellobiohydrolase I (Cel7A), cellobiohydrolase II (Cel6A), and endoglucanase I (Cel7B). The interference from substrate materials present in lignocellulosic supernatants could be minimized by dilution. Conclusion: A double-antibody sandwich ELISA was able to detect and quantify individual enzymes when present in cellulase mixtures. The assay was sensitive over a range of relatively low enzyme concentration (0 -1 μg/ml), provided the enzymes were first pH adjusted and heat treated to increase their antigenicity. The immunoassay was employed to quantitatively monitor the adsorption of cellulase monocomponents, Cel7A, Cel6A, and Cel7B, that were present in both Celluclast and Accellerase 1000, during the hydrolysis of steam-pretreated corn stover (SPCS). All three enzymes exhibited different individual adsorption profiles. The specific and quantitative adsorption profiles observed with the ELISA method were in agreement with earlier work where more labour intensive enzyme assay techniques were used.

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Optimization of Steam Pretreatment of SO2-Impregnated Corn Stover for Fuel Ethanol Production

Cited by 20 publications

References 14 publications

Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method

Ethanol production from corn stover pretreated by electrolyzed water and a two-step pretreatment method

Perspectives for the Production of Bioethanol from Lignocellulosic Materials

The Development and Use of an Elisa-Based Method to Follow the Distribution of Cellulase Monocomponents During the Hydrolysis of Pretreated Corn Stover

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