Optimization of somatic embryogenesis, synthetic seed production, and evaluation of genetic fidelity in Teucrium polium L.

Saadat, Saeed; Majd, Ahmad; Naseri, Lotfali; Iranbakhsh, Alıreza; Jafari, Morad

doi:10.1007/s11627-023-10360-6

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…The balance between these two solutions is fundamental, and the ion exchange between them varies depending on the explant and the species to be encapsulated [32]. At concentrations of less than 3% for sodium alginate and less than of 100 mM for CaCl 2 , the alginate beads in Teucrium polium L. were very loose and unable to cover the plant tissue, while concentrations higher than 3% resulted in the darkening of plant tissues and the absence of germinated plants [33]. Concentrations of 3% for sodium alginate and 100 mM for CaCl 2 were chosen for the production of synthetic seeds of I. pallida species, effectively facilitating the formation of alginate beads coating the plant tissues without negatively impacting the regrowth of somatic embryos.…”

Section: Discussionmentioning

confidence: 99%

Synthetic Seed Production and Slow Growth Storage of In Vitro Cultured Plants of Iris pallida Lam.

Meucci,

Ghelardi,

Chietera

et al. 2024

Horticulturae

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Iris pallida Lam. is traditionally cultivated in Italy to sell its rhizomes to perfume-producing industries and is particularly sought-after because of its high content of irones, ketone compounds responsible for the violet smell of the orris essence. One of the critical aspects of its cultivation is the propagation method, performed by subdividing and replanting sections of the rhizome, which leads to the sacrifice of salable material. A solution is provided via in vitro propagation using the somatic embryogenesis technique, an effective method that allows the production of plants without the use of the rhizome. To facilitate the scale up of the activities of micropropagation companies, the method of slow growth storage (SGS) for orris plantlets and a somatic embryo encapsulation technique were developed for the first time. Orris plantlets were placed at 4 °C in the dark for 30, 60, 90 and 120 days and monitored 7 and 30 days after treatment. Synthetic seeds were obtained by encapsulating somatic orris embryos in sodium alginate beads, which were stored for 14 and 28 days at 4 °C and 24 °C. The results showed that it is possible to cold-preserve orris plantlets for up to 90 days without significant damages and that orris synthetic seeds can be produced and stored for a short-to-mid-term period. These conservation techniques can be useful for germplasm conservation and can also be integrated in the micropropagation cycle of orris, helping to solve issues related to the traditional propagation method.

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Section: Discussionmentioning

confidence: 99%

Synthetic Seed Production and Slow Growth Storage of In Vitro Cultured Plants of Iris pallida Lam.

Meucci,

Ghelardi,

Chietera

et al. 2024

Horticulturae

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“…ISSRs consist of DNA fragments ranging from 100 to 3000 base pairs, which are located between adjacent, inversely oriented microsatellite regions. These sequences are ampli ed based on polymerase chain reactions with 16-18 base pairs single primers derived from microsatellite core sequences, as well as based on a few selective nucleotides extending into adjacent non-repeat regions (Godwin et al, 1997) Saadat et al, 2023). This underscores the importance of ISSR markers as reliable tools that can be utilized for genetic assessment in the eld of plant tissue culture and propagation.…”

Section: Introductionmentioning

confidence: 99%

The Comprehensive In Vitro Propagation and Genetic Homogeneity Analysis of Cryptocoryne crispatula var. yunnanensis: Addressing the Conservation Concerns for an Endangered Species in China and the Mekong Basin

Sakulsathaporn,

Mapanao

2024

Preprint

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This study focused on the propagation of the endangered aquatic plant species Cryptocoryne crispatula var. yunnanensis, threatened by hydropower dam construction in Thailand and classified as endangered in China. The objective was to propagate the species using shoot explants. A sterilization method with a 45.45% success rate involved treating shoots with 0.01% HgCl2 for 90 minutes and 5% commercial bleach (6% NaOCl) for 20 minutes. The study investigated the effect of Murashige and Skoog (MS) medium, supplemented with 0.5 mg/L NAA and 1-4 mg/L cytokinins (BA, kinetin, and TDZ), on shoot initiation and proliferation. TDZ was found to be more effective than BA and kinetin in enhancing shoot growth. The optimal shoot induction, averaging 7.14 shoots per explant, occurred in MS medium with 0.5 mg/L NAA and 3 mg/L TDZ. A medium of 0.5 mg/L NAA and 1 mg/L TDZ significantly increased shoot proliferation, yielding an average of 23.75 shoots per explant. The most successful ex vitro rooting and acclimatization method involved 1X vitamin stock MS medium with 0.5 mg/L IBA, followed by transfer to plastic pots with a 1:1 sand and vermiculite mix, achieving a 73.33% survival rate and an average of 6.31 roots per explant. Genetic uniformity and stability of the propagated clones were verified using ISSR markers. This protocol enhances the conservation efforts for C. crispatulavar. yunnanensis by supporting its multiplication and preservation in synthetic habitats.

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“…Somaclonal variation is a frequent occurrence in plant tissue culture, necessitating a comparison of the regenerated plants' genetic faithfulness to their donor mother plants (Bahmankar et al 2017). A few of the reported reasons for the phenomenon of somaclonal variety include changes in DNA methylation, cytogenetic diversity, adjustments to the DNA sequence, and epigenetic changes (Bahmankar et al 2017;Saadat et al 2023). Molecular markers are primarily a useful method for assessing genetic delity at the DNA level, but because these techniques are ineffective at detecting changes in ploidy level and cytogenetic variety, ow cytometric analysis is more effective.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and genetic fidelity in camelina by RAPD markers and flow cytometry

Bahmankar,

Rahnama,

Salehi

et al. 2024

Plant Cell Tiss Organ Cult

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Camelina (Camelina sativa L. Crantz) is an oily, medicinal plant that is a member of the Brassicaceae family. It has a lot of important agronomic characteristics, including as good environmental adaptability and tolerance to cold, heat, pests, and diseases. The present investigation aimed to improve somatic embryogenesis, and camelina regeneration, and evaluate genetic stability in the regenerated plantlets using RAPD markers and ow cytometry. Two explants of the hypocotyl and cotyledon were used, together with four different combinations of PGRs made up of NAA, BAP, 2,4-D, and Kin, to optimize somatic embryogenesis and regeneration in Camelina. Because several somatic embryogenesis developmental phases could be observed on embryogenic calluses at the same time, the results suggested that somatic embryogenesis in camelina is a simultaneous occurrence. The greatest rate of somatic embryogenesis and regeneration was seen in the cotyledon explant grown in the MS + 0.3 mgL-1 NAA + 0.7 mgL-1 BAP. The results also revealed that the MS + 0.25 mgL − 1 IAA + 0.5 mgL − 1 NAA had the best rooting response and a favorable seedling survival rate. In the present work, RAPD markers were utilized for the rst time in camelina to con rm the genetic delity of in vitro regenerated plants and their donor mother plant. The ampli ed products showed 64 different, scorable bands, and the regenerated plants were an identical replica of their donor mother plants. The delity of the ploidy level was assessed by ow cytometry, and the ndings con rmed monomorphic patterns in both the regenerated plants and their donor mother plants. According to the present ndings, it can be said generally that somatic embryogenesis may be advantageous for large-scale multiplication, breeding programs, and in vitro conservation in camelina. Key MessageIt has been shown that the type of auxin and cytokinin employed during camelina embryogenesis and regeneration has a signi cant in uence on the procedure. It is better for these operations to use a cotyledon explant. In camelina, somatic embryogenesis occurs concurrently, and the genetic similarity of the donor mother plant and the in vitro regenerated plants was con rmed using RAPD markers and ow cytometry.

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Optimization of somatic embryogenesis, synthetic seed production, and evaluation of genetic fidelity in Teucrium polium L.

Cited by 5 publications

References 51 publications

Synthetic Seed Production and Slow Growth Storage of In Vitro Cultured Plants of Iris pallida Lam.

Synthetic Seed Production and Slow Growth Storage of In Vitro Cultured Plants of Iris pallida Lam.

The Comprehensive In Vitro Propagation and Genetic Homogeneity Analysis of Cryptocoryne crispatula var. yunnanensis: Addressing the Conservation Concerns for an Endangered Species in China and the Mekong Basin

Somatic embryogenesis and genetic fidelity in camelina by RAPD markers and flow cytometry

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