Somatic embryogenesis and genetic fidelity in camelina by RAPD markers and flow cytometry

Abstract

Camelina (Camelina sativa L. Crantz) is an oily, medicinal plant that is a member of the Brassicaceae family. It has a lot of important agronomic characteristics, including as good environmental adaptability and tolerance to cold, heat, pests, and diseases. The present investigation aimed to improve somatic embryogenesis, and camelina regeneration, and evaluate genetic stability in the regenerated plantlets using RAPD markers and ow cytometry. Two explants of the hypocotyl and cotyledon were used, together with four different combinations of PGRs made up of NAA, BAP, 2,4-D, and Kin, to optimize somatic embryogenesis and regeneration in Camelina. Because several somatic embryogenesis developmental phases could be observed on embryogenic calluses at the same time, the results suggested that somatic embryogenesis in camelina is a simultaneous occurrence. The greatest rate of somatic embryogenesis and regeneration was seen in the cotyledon explant grown in the MS + 0.3 mgL-1 NAA + 0.7 mgL-1 BAP. The results also revealed that the MS + 0.25 mgL − 1 IAA + 0.5 mgL − 1 NAA had the best rooting response and a favorable seedling survival rate. In the present work, RAPD markers were utilized for the rst time in camelina to con rm the genetic delity of in vitro regenerated plants and their donor mother plant. The ampli ed products showed 64 different, scorable bands, and the regenerated plants were an identical replica of their donor mother plants. The delity of the ploidy level was assessed by ow cytometry, and the ndings con rmed monomorphic patterns in both the regenerated plants and their donor mother plants. According to the present ndings, it can be said generally that somatic embryogenesis may be advantageous for large-scale multiplication, breeding programs, and in vitro conservation in camelina. Key MessageIt has been shown that the type of auxin and cytokinin employed during camelina embryogenesis and regeneration has a signi cant in uence on the procedure. It is better for these operations to use a cotyledon explant. In camelina, somatic embryogenesis occurs concurrently, and the genetic similarity of the donor mother plant and the in vitro regenerated plants was con rmed using RAPD markers and ow cytometry.