Optimization of Somatic Embryogenesis Protocol for Locally Adapted Eggplant (Solanum melongena L.) Varieties in Bangladesh

Habib, M. A. B.; Muktadir, Abdul; Kabir, Muhammad Rezaul; Mian, MA Khaleque; Akhond, MA Yousuf

doi:10.3329/ptcb.v26i1.29769

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…(Poaceae) with 2,4-D (18.1 μM) [ 43 ]; Musa acuminata Colla (Musaceae) with 2,4-D (4.5 μM) [ 44 ]; Salicornia brachiata Roxb. with 2,4-D (2.0 mg L −1 ) [ 45 ], and Solanum melongena L. (Solanaceae) with 2,4-D (0.5 mg L −1 ) [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L−1), Naphthaleneacetic Acid (NAA) (1 mg L−1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L−1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L−1) and NAA (1 mg L−1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L−1) and NAA (0.25, 0.50, and 1 mg L−1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L−1 of BAP and 1 mg L−1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L−1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

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Section: Resultsmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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“…Following the initial reports of Reinert [6] and Steward et al [7], the phenomenon of somatic embryogenesis was reported in a number of medicinal plants: Solanum melongena [8,9,10,11], Solanum quitoense [12] Solanum lycopersicum [13], Tribulus terrestris [14], Psoralea corylifolia [15], S. surattense [5] and Senna alata [16].…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration via Somatic Embryogenesis in Solanum nigrum L. (Black nightshade) (Solanaceae)

Sharada

Krishna

Swamy

2019

BJI

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Aim: To study the effect of various plant growth regulators (PGRs) for induction of somatic embryogenesis and plantlet formation from cotyledon and leaflet explants in S. nigrum (night shade) an important medicinal plant used in treatment of digestive problems and skin infections. Place and Duration of Study: Department of Biotechnology, Kakatiya university, Warangal. Telangana, India, 3 years. Methodology: Cotyledon (0.8 cm2) and leaflet explants (0.8-1.0 cm2) from 3 week and 4 week old were cultured on MS medium supplemented with 30 g/L sucrose along with different concentrations of 0.5 mg/L BAP+NAA (0.5 – 6.0 mg/L) . Results: Maximum percentage of somatic embryogenesis was observed in cotyledon(89%) and leaf (98%) explants on MS medium augmented with 0.5mg/L BAP in combination with 2.0 mg/L NAA whereas the highest number of somatic embryos per explant (86 ± 0.19) was formed in leaflet explant. Conclusion: Somatic embryogenesis was induced from both cotyledon and leaf explants. Since it is threatened and medicinally important species S. nigrum, the present protocol can be used for its conservation and genetic transformation experiments.

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Genetically Modified Brinjal (Solanum melongena L.) and Beyond

Chadipiralla

Pullaiah

Rajam

2020

Genetically Modified Crops

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Optimization of Somatic Embryogenesis Protocol for Locally Adapted Eggplant (Solanum melongena L.) Varieties in Bangladesh

Cited by 4 publications

References 13 publications

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Plant Regeneration via Somatic Embryogenesis in Solanum nigrum L. (Black nightshade) (Solanaceae)

Genetically Modified Brinjal (Solanum melongena L.) and Beyond

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