Plant Regeneration via Somatic Embryogenesis in Solanum nigrum L. (Black nightshade) (Solanaceae)

Sharada, Durgam; Krishna, Porandla Sai; Swamy, N. Rama

doi:10.9734/bji/2019/v23i130070

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…It's also interesting to note that BAP outperformed KIN at causing many shoots in S. torvum, either on its own or in combination with IAA. The similar superiority of BAP in shoot meristem cultures was shown in other Solanaceous medicinal plants as well, including S. surattense (Rama Swamy et al, 2005) and S. nigrum (Sharada et al, 2019). Merclone technology is a terrific way to speed up in vitro multiplication and cultivate virus-free and pathogen-free plants.…”

Section: B Effect Of Kin and Iaa+kinmentioning

confidence: 62%

“…Somatic embryogenesis can be induced directly from a variety of explants or indirectly through In vitro manipulation of non-embryogenic callus. Cell totipotency is best demonstrated in the formation of somatic embryos from single cells, as well as their growth and development to form a complete plantlet (Ammirato, 1983;Rama Swamy et al, 2005;Sharada et al, 2019;Ghan Singh et al, 2021a). Because somatic embryos are thought to be formed from a single cell, plants derived from them are genetically identical.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…Genotype is the most important factor influencing somatic embryogenesis, with significant quantitative differences in their ability to form embroids in S. melongena,S. nigrum and S. torvum (Huda et al, 2007;Mir et al, 2008;Mir et al, 2011;Sidhu et al, 2014;Sharada et al, 2019;Ghan Singh et al, 2021a).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…Recently, Rama Swamy et al (2005) reported that both auxin-cytokinin combinations are required for inducing somatic embryogenesis in S.surattense, a medicinal plant. Direct somatic embryogenesis was induced on medium containing BAP in S. nigrum, and the number of embryos was increased further by enriching the medium with NAA (Sharada et al, 2019). In comparison to cotyledon explants, leaf explants produced the highest frequency number of somatic embryos and converted the most into bipolar embryos at 5.0 mg/L BAP+2.5 mg/L NAA (Ghan Singh et al, 2021a).…”

Section: Somatic Embryo Germination and Plantlet Formationmentioning

confidence: 99%

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A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Maloth¹,

Marka

Nanna

2023

EJBIO

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In this review, we report on the different plant growth regulators (PGRs) affecting the callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from different explants and micropropagation through shoot tip and nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of this plant guard against liver and kidney damage, stop certain cancers, and regulate blood sugar levels. They benefit digestion, the management of gout and menstruation, the treatment of anaemia and diabetes, the prevention of intestinal worms, cardiovascular disorders, and strokes. The protocol developed for the induction of callus can be utilized to isolate pharmaceutically important secondary metabolites in S. torvum, and the regeneration protocols optimized in this study can be used for genetic transformation and conservation of S. torvum, not only as a medicinal plant but also a model system.

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Section: B Effect Of Kin and Iaa+kinmentioning

confidence: 62%

Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Somatic Embryo Germination and Plantlet Formationmentioning

confidence: 99%

See 2 more Smart Citations

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Maloth¹,

Marka

Nanna

2023

EJBIO

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show abstract

“…Callus mediated regeneration was reported in medicinal plants such as Momordica dioica (Hoque et al, 2000), Solanum species (Gleddie et al, 1983;Baburaj & Gunasekaran, 1994;Shahzad et al, 1999, Ugandhar et al, 2006, Sharada et al, 2019. Though the plant Turkey Berry is ethnomedicinally important, but there is no report on callus mediated regeneration.…”

Section: Introductionmentioning

confidence: 99%

High Frequency Callus Mediated Plantlet Regeneration from Different Explants of Ethno-medicinal Plant Turkey Berry (Solanum torvum Sw)

Malothu¹,

Marka²,

Nanna³

2022

JSR

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We report on the influence of different plant growth regulators (PGRs) as well as the type of explants on callus mediated regeneration in Turkey berry/pea egg medicinal plant, S.torvum. The explants viz., hypocotyl (0.4-0.8 cm long), cotyledon (0.6-0.8 cm 2 ) from 3 week old and leaves (1.0 cm 2 ) from 6 week old in vitro grown seedlings were cultured on MS medium with various concentrations (0.5-4.0 mg/L) of BAP/KIN alone and also in combination with 0.5 mg/L NAA/IAA. The calli developed from all the explants have shown the maximum percentage of response (94% leaf and cotyledon, 88% hypocotyl) and highest number of multiple shoots induction (36.2±1.50 leaf, 33.2±0.25 cotyledon and 23.4±0.11 hypocotyl explants) per explant with maximum length of shoots (3.9±0.18 leaf, 4.0±0.11 cotyledon and 3.7±0.19 hypocotyl) at 0.5 mg/L NAA+2.5 mg/L BAP (leaf), 0.5 mg/L IAA+2.5 mg/L BAP (cotyledon) and 0.5 mg/L IAA+3.0 mg/L BAP (hypocotyl) in comparison to different concentrations of cytokinins used alone and as well as in combination with IAA/NAA. The calli induced from cotyledon explants were found to be more potent in inducing high frequency number of shoots per explant among all other explants tested in the present investigations. Cytokinins BAP/KIN alone or in combination with IAA/NAA was found to be more effective in inducing callus mediated shoot regeneration in all the explants of S. torvum. For in vitro rooting, the elongated microshoots were transferred on to root induction medium (RIM) fortified with 0.25-2.0 mg/L NAA/IAA. Maximum percentage of response (90%), average number of roots (18.2±0.03) per micro-shoot with highest length of roots (7.8±0.09 cms) was observed at 1.0 mg/L IAA. In vitro rooted plantlets were acclimatized in the green house and transferred into field. The survival percentage was found to be 90% and the plantlets were found to be normal in morphology, flowering and fruiting. Thus, the regeneration protocol developed in the present investigation can be used for the conservation and genetic transformation experiments in S. torvum, not only as a medicinal plant but also as a model plant, based on its regeneration potentiality.

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A convenient, reliable and directly visual selection marker for identifying transgenic lines of Solanum nigrum

Peng

Wang

Wei

et al. 2022

Plant Cell Tiss Organ Cult

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Plant Regeneration via Somatic Embryogenesis in Solanum nigrum L. (Black nightshade) (Solanaceae)

Cited by 7 publications

References 15 publications

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

High Frequency Callus Mediated Plantlet Regeneration from Different Explants of Ethno-medicinal Plant Turkey Berry (Solanum torvum Sw)

A convenient, reliable and directly visual selection marker for identifying transgenic lines of Solanum nigrum

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