Optimization of solid-phase extraction using artificial neural networks in combination with experimental design for determination of resveratrol by capillary zone electrophoresis in wines

Spanilá, Miroslava; Pazourek, Jiří; Farková, Marta; Havel, Josef

doi:10.1016/j.chroma.2004.10.007

Cited by 30 publications

(15 citation statements)

References 14 publications

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“…Taking into account the physicochemical properties of the target compounds, high-performance liquid chromatography (HPLC) is the technique most commonly used, employing different detection modes like ultraviolet or diode array (Cvejic et al 2010;Zhou et al 2007;Du et al 2007;Enguo et al 2007;Abert Vian et al 2005), chemiluminescence (Zhou et al 2004), electrochemistry (Zhang et al 2007; Kahoun et al 2008), fluorimetry (Grippi et al 2008;Rodríguez-Delgado et al 2001), or mass spectrometry (Sun et al 2006;Hamm et al 2010;Jaitz et al 2010;Nicoletti et al 2007;Counet et al 2006;Montsko et al 2008). As an alternative to HPLC, gas chromatography-mass spectrometry (GC-MS) (Soleas et al 2001;Luan et al 2000) and capillary electrophoresis (Spanilá et al 2005;Zhang et al 2009;Orlandini et al 2008) have also been employed.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

et al. 2011

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In this work, a liquid chromatography method with diode array and fluorescence detection and electrospray ionization mass spectrometry (LC-DAD-FLD and LC-ESI-MS) has been developed for the determination of residues of resveratrol in all its forms (free isomers and glycosylates) in honey. This procedure involves a solidphase extraction on polymeric cartridges for the isolation of trans/cis-isomers of resveratrol and piceid from diluted honey samples. Chromatographic separation of the analytes was performed in isocratic mode on a C 18 column (150 mm×4.6 mm i.d., 5 μm) operated at 30°C. The mobile phase consisted of a mixture of water with 1% formic acid and acetonitrile (75/25, v/v), and the flow rate was set at 1.0 mL/min. Average analyte recoveries were from 71% to 113% in replica sets of fortified honey samples. The detection limits when using LC-DAD-FLD were between 40 and 120 μg/kg, meanwhile for LC-ESI-MS were between 1.4 and 14.6 μg/kg. The proposed method was applied to the analysis of honey samples collected from treated beehives in experimental apiaries after a preliminary field trial, and they were not found any residues over the detection limits of the studied compounds.

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Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

et al. 2011

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“…Eventually, the experiment is done using the best predictable condition. This methodology has been described first in [10] and has been cited in many other papers referenced in the manuscript [11,12].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Salvia officinalis plant extracts by capillary electrophoresis

Hameda¹,

Gajdošová

Havel

2006

J of Separation Science

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Salvia officinalis (commonly called Sage) and similar plants contain many compounds of pharmaceutical interest and are used as a tea or in various pharmaceutical products. In this work, the use of CE for analysis of aqueous or ethanolic extracts from various Salvia plants has been studied. Especially, several buffers like borate, phosphate, acetate, etc., were examined under different concentrations, pH, separation voltage, injection time, and other parameters to find the optimal separation conditions. The optimization was also performed using experimental design and artificial neural networks. The optimal conditions were: separation voltage +20 kV, 40 mM buffer borate, pH 9.2, injection time 5 s, and UV detection at 280 nm. A new CE method has been developed, validated, and applied to analyze samples of S. officinalis from various countries.

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“…Detection based on electrochemistry, luminescence, and MS offers higher sensitivity than DAD. Gas chromatography tandem MS, previous derivatization, normally with bis(trimethylsilyl)trifluoroacetamide (BSTFA), before column application [27], and micellar electrokinetic chromatography (MEKC) with UV-detection [28], capillary zone electrophoresis (CZE), with DAD [29] or ECD [30], or non-aqueous CZE with DAD [31] have been also employed for the analysis of resveratrol. Piceid has been also analyzed by CZE, with DAD [32].…”

Section: Introductionmentioning

confidence: 99%

Isocratic chromatography of resveratrol and piceid after previous generation of fluorescent photoproducts: Wine analysis without sample preparation

Galeano-Dı́az

Durán-Merás

Airado‐Rodríguez

2007

J of Separation Science

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Resveratrol and its 3-glucoside (piceid), are stilbene-like molecules produced by plants. Both of them are weakly fluorescent, but highly fluorescent compounds are obtained when their hydroethanolic solutions are UV-irradiated, which implies a substantial improvement in the sensitivity of analytical methods. Experimental design (central composite design) together with the response surface methodology have been used to find optimum conditions for the fast, sensitive, and precise chromatographic analysis (with isocratic elution) of resveratrol and piceid in wine samples. These compounds have been UV-transformed into their respective photoproducts, which have been separated in a C18 column (Novapack C(18) 150x3.9 mm, 4 microm) by isocratic elution, using a mobile phase made up of acetonitrile and 4.1 vol% aqueous acetic acid, 19:81 v/v, at a flow rate of 0.8 mL/min, and fluorimetrically detected at 364 nm (lambda(exc) = 260 nm). Detection limits (S/N = 3) are 0.29 and 0.28 microg/L for resveratrol and piceid, respectively. The method has been applied to the analysis of these compounds in wine samples without a clean-up step. The analysis is completed in only 20 min. The standard addition method has been applied to the analysis of a commercial red wine and average recoveries near 100% were obtained for resveratrol and piceid. Three wine pools were satisfactorily analysed by external calibration.

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Optimization of solid-phase extraction using artificial neural networks in combination with experimental design for determination of resveratrol by capillary zone electrophoresis in wines

Cited by 30 publications

References 14 publications

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

Liquid Chromatographic Determination of Resveratrol and Piceid Isomers in Honey

Analysis of Salvia officinalis plant extracts by capillary electrophoresis

Isocratic chromatography of resveratrol and piceid after previous generation of fluorescent photoproducts: Wine analysis without sample preparation

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