Analysis of Salvia officinalis plant extracts by capillary electrophoresis

Abstract

Salvia officinalis (commonly called Sage) and similar plants contain many compounds of pharmaceutical interest and are used as a tea or in various pharmaceutical products. In this work, the use of CE for analysis of aqueous or ethanolic extracts from various Salvia plants has been studied. Especially, several buffers like borate, phosphate, acetate, etc., were examined under different concentrations, pH, separation voltage, injection time, and other parameters to find the optimal separation conditions. The optimization was also performed using experimental design and artificial neural networks. The optimal conditions were: separation voltage +20 kV, 40 mM buffer borate, pH 9.2, injection time 5 s, and UV detection at 280 nm. A new CE method has been developed, validated, and applied to analyze samples of S. officinalis from various countries.

Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule

“…Thus, the selection of a suitable method to optimize the separation conditions is important. There are many approaches available for the optimization of separation conditions, such as experimental design coupled with response surface method, multivariate analysis, or artificial neural network method (Ben Hameda et al, 2006;Lin, 2006;Shaban et al, 2006;Hanrahan et al, 2008;Liu et al, 2009). Central composite design (CCD), one of experimental design methods, has been widely used to optimize the separation conditions of CE, owing to its simple procedure, fewer experimental runs, and its advantage in the full evaluation on interactions of factors (Lin, 2006;Liu et al, 2006;Yu et al, 2006a;2006b;2006c;2007;Liu et al, 2008).…”

Characterization of phenolic and other polar compounds in a lemon verbena extract by capillary electrophoresis‐electrospray ionization‐mass spectrometry

“…In the last decade, CE has gained widespread interest as a valuable tool for the determination of bioactive compounds in plant matrices 18–24. This increasing interest is due to its attractive advantages such as high speed of analysis, the small sample amounts required and extremely limited solvent waste 25.…”

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

“…The wide use of UV detection was also true for food and food contaminants analyses 85, 177, 190, 196–287. However, increasing also were those exploiting CE‐MS 141, 143–145, 146, 148, 288–303.…”

“…A large number of research articles 141–145, 146, 148, 154, 161, 162, 177, 190, 195–309, 336, 337, 376–384 on CE of food/food contaminants came out during the 2006–2010 coverage. Most of these were on the determination of polyphenolic compounds, the most widely distributed secondary metabolites in plants.…”