Optimization of separations of alkyl‐substituted phenolate anions by capillary zone electrophoresis

Benz, Nancy J.; Fritz, James S.

doi:10.1002/jhrc.1240180308

Cited by 21 publications

(9 citation statements)

References 13 publications

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“…This is attributed to the decrease of thickness of electrical double-layer and zeta potential in a high salt concentration buffer [30]. The EOF decreased from 0.652 to 0.562 mm/s when ACN content in the mobile phase increased from 50 to 95%, which was mainly due to a collective action of the decreasing dielectric constant and viscosity and the decreasing ionic strength (raising the trend of zeta potential) [31].…”

Section: Studies Of Eofmentioning

confidence: 99%

Dipyridyl‐immobilized ionic liquid type hybrid silica monolith for hydrophilic interaction electrochromatography

Wang

Huang

Wang³

et al. 2013

Electrophoresis

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A pyridinium-based immobilized ionic liquid type multifunctional hybrid silica monolith was prepared by the in situ polymerization of 3-chloropropyl-silica matrix and 4,4'-dipyridyl for hydrophilic interaction CEC. The obtained hybrid monolith possessed of high stable skeletal microstructures with obviously hydrophilic retention mechanism under ACN content >50% in the mobile phase. Strong and stable anodic EOF could be observed under a broad pH range from pH 3.0 to 9.0. Due to the immobilized dipyridyl groups bonded to the silica matrix surface, the resulting hydrophilic hybrid monolith possessed multiple separation interactions including hydrogen bond, π-π, and anion exchange. Excellent separations of various polar analytes including electroneutral phenols, charged acid nucleotides, and basic analytes were successfully achieved. The highest column efficiencies up to 120,000, 164,000, and 106,000 plates/m were obtained for nucleotides, nucleic acid bases, and nucleosides and nicotines, respectively. These results demonstrated that the dipyridyl-immobilized ionic liquid functionalized hybrid monolith possessed highly mechanical stability and good chromatographic performance for hydrophilic interaction electrochromatography.

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Section: Studies Of Eofmentioning

confidence: 99%

Dipyridyl‐immobilized ionic liquid type hybrid silica monolith for hydrophilic interaction electrochromatography

Wang

Huang

Wang³

et al. 2013

Electrophoresis

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“…In addition, a decrease in observed mobility of the test solutes with increasing concentration was observed. This can be attributed both to a dynamic equilibrium in which organic modifier and TTAB are adsorbed onto the capillary surface and to structural changes of the hemimicelle itself [23]. Therefore, considering both separation time and resolution, 15% acetonitrile (v/v) was added to the electrolyte.…”

Section: Mobilitymentioning

confidence: 99%

Application of co-electroosmotic capillary electrophoresis for the determination of inorganic anions and carboxylic acids in soil and plant extract with direct UV detection

Naidu

Chen

2001

Chromatographia

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SummaryIndirect UV detection in capillary zone electrophoresis (CZE) is frequently used for the determination of inorganic anions and carboxylic acids. However, there are few reports on direct UV detection of these solutes in real samples. This paper describes the use of direct UV detection of inorganic anions and organic acids in environmental samples using co-electroosmotic capillary zone electrophoresis (co-CZE) at 185 nm. The best separation and detection of the solutes was achieved using a fused silica capillarywith an electrolyte containing 25 mM phosphate, 0.5 mM tetradecyltrimethylammonium bromide (gAB) and 15% acetonitrile (v/v) at pH 6.0. Four common inorganic anions (CI-, NO2-, NO3-and SO42-) and 11 organic acids (oxalic, formic, fumaric, tartaric, malonic, malic, citric, succinic, maleic, acetic, and lactic acid), were determined simultaneously in 15 min. Linear calibration plots for the test solutes were obtained in the range 0.02 -0.5 mM with detection limits ranging from 1 -9 I~M depending on the analyte. The proposed method was successfully used to determine inorganic anions and carboxylic acids in soil and plant tissue extracts with direct injection of the sample.

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“…In most cases these problems can be overcome by increasing the ionic strength of the buffer or by adding organic solvents to the electrolyte [35,36,38,39]. This paper describes the migration behavior of carbohydrates derivatized with ethyl p-aminobenzoate or p-aminobenzonitrile under co-electroosmotic conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The concept of co-electroosmotic migration of anions has been successfully used for various applications, such as the separation of inorganic anions [31][32][33][34], phenolic compounds [35][36][37][38][39] and carboxylic acids [40]. Modification of the EOF has also been used in the capillary electrophoretic separation of carbohydrates [21,42].…”

Section: Introductionmentioning

confidence: 99%

Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis

et al. 1997

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SummaryThe separation of derivatized carbohydrates has been performed by co-electroosmotic capillary electrophoresis. Derivatization was performed by reductive amination of the carbohydrates with ethyl p-aminobenzoate or with p-aminobenzonitrile. Separation selectivity is optimized using buffer electrolytes containing high concentrations of borate, organic solvents, and mixtures thereof; this enabled separation of the carbohydrate derivatives then direct UV detection. Co-directional migration of the anionic analytes with the electroosmotic flow was achieved by adding a cationic polymer (hexadimethrine bromide, HDB) to the electrolyte. With this method it is possible to determine specific carbohydrates, such as arabinose, mannose, and glucose, which are difficult to separate by other CE methods. The applicability of the method is demonstrated for the analysis of plant hydrolyzates

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Optimization of separations of alkyl‐substituted phenolate anions by capillary zone electrophoresis

Cited by 21 publications

References 13 publications

Dipyridyl‐immobilized ionic liquid type hybrid silica monolith for hydrophilic interaction electrochromatography

Dipyridyl‐immobilized ionic liquid type hybrid silica monolith for hydrophilic interaction electrochromatography

Application of co-electroosmotic capillary electrophoresis for the determination of inorganic anions and carboxylic acids in soil and plant extract with direct UV detection

Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis

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