Optimization of purification procedure for horse F(ab´)2 antivenom against &lt;i&gt;Androctonus crassicauda&lt;/i&gt; (Scorpion) venom

Taherian, Azadeh; Fazilati, Mohammad; Moghadam, Ahmad Taghavi; Tebyanian, Hamid

doi:10.4314/tjpr.v17i3.4

Cited by 20 publications

(13 citation statements)

References 10 publications

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“…Then, the adipose tissue was transferred into a solution of salt-buffered saline and 0.3% collagenase (SERVA, Germany) for 45 -60 minutes at 37°C, allowing the whole tissue to completely digest. After digestion, the enzyme was neutralized by adding cold buffer solution (PBS) (MiltenyiBiotec, Germany) and centrifugation was performed (25)(26)(27). The supernatant containing debris and fat droplets was discarded.…”

Section: Isolation and Culture Of Mesenchymal Stem Cells Of Rat Adipomentioning

confidence: 99%

Effect of Adipose-Derived Mesenchymal Stem Cell Repair in Nicotine Model Rat: A Femoral Bone Diaphyseal Defect Study

Riahi

Parivar

Baharara

et al. 2020

Iran Red Crescent Med J

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Background: The major function of the bone in the skeletal system is to provide structural support to the body and its vital organs. Many patients suffer from the disability to restore bone lesions following bone fractures during crashes or accidents. The use of mesenchymal stem cells such as adipose-derived mesenchymal stem cells (ADMSCs), along with collagen scaffolds, and its transfer to the lesion site can be valued as one of the available treatment options. Objectives: In the current paper, a study was conducted on the level of mesenchymal stem cell repair from the rat adipocytes, where it was evaluated in bone defects. Methods: In this study, mesenchymal stem cells were isolated from the rat adipocytes and their stem cell lines were determined with the standard cell tests. The isolated cells were differentiated in the next step and transferred to the two main groups: nicotine modeled and non-modeled (non-nicotine) along with collagens. The repair of the defect caused by a 2 mm drill in the diaphyseal region of the rat bone was evaluated after four weeks using radiographic examination and histopathologic staining. Results: Radiographic data analysis indicated that bone density was much higher in the non-nicotine group than in the nicotine group. Histopathologic staining showed that bone formation was higher in the non-nicotine group than in the nicotine group. The new bone formation was about 80% and 60% in the non-nicotine and nicotine groups with differentiated osteocytes of ADMSCs, respectively. Conclusions: Adipose-derived mesenchymal stem cell transplantation is effective in bone defect repair and nicotine plays an important role in the bone repair process as an inhibitory agent.

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Section: Isolation and Culture Of Mesenchymal Stem Cells Of Rat Adipomentioning

confidence: 99%

Effect of Adipose-Derived Mesenchymal Stem Cell Repair in Nicotine Model Rat: A Femoral Bone Diaphyseal Defect Study

Riahi

Parivar

Baharara

et al. 2020

Iran Red Crescent Med J

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“…The primers were designed using NCBI and Primer 3 software and synthesized by Sinaclon Co. (Iran) in lyophilized form. During PCR reaction (19,20), primers are bonded on both sides of the target sequence and allow the polymerase activity of DNA polymerase. Therefore, the most important criterion for selecting a primer is that of attention to the matching of sequence of primer with template.…”

Section: Pcr Confi Rmation Of Differentiation Of Bone Marrow Mesenchymentioning

confidence: 99%

Evaluation of the repair of diaphyseal fracture of femoral bone using bone marrow mesenchymal stem cells in nicotine-bearing rat

Riahi¹,

Parivar²,

Baharara³

et al. 2019

BLL

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OBJECTIVE: Following bone fractures during accidents, some patients suffer from poor repair of bone fractures and subsequent aesthetic and psychological problems. One of the treatments is based on transplantation of stem cells (seeded on scaffolds) to the lesion site. Bone marrow stromal cells (BMSCs) are multivalent stem cells which are able to reproduce and differentiate into osteogenic cells. The objective of this study was to evaluate the treatment of bone fractures by means of transplantation of the latter cells in rats. METHODS: In this study, the therapeutic effect of mesenchymal stem cells from bone marrow adipocytes was evaluated in bone fractures. BMSCs were isolated from rat femur. Two sources of differentiated and non-differentiated osteocyte cells were provided and mixed with collagen in order to be transferred to animals divided in three main groups of model: nicotine, non-nicotine and control groups. After four weeks, the repair of the fracture that had been infl icted by a 2-mm drill into the diaphyseal region of the femoral bone was investigated by radiographic tests and histopathologic staining. RESULTS: The radiographic results as well as those of histopathologic staining showed that osteogenesis was more intensive in the non-nicotine group than in the nicotine group with differentiated and non-differentiated osteocyte cells. CONCLUSION: The transplantation of differentiated BMSCs to a bone lesion affected the repair of bone fractures while the nicotine agent played an important role in delaying the bone regeneration (Tab. 1, Fig. 8, Ref. 31).

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“…A sterile swab was dipped into the suspension and then inoculated on Muller-Hinton agar (Merck, Germany) plate in order to provide a uniform coverage of bacteria on the surface of the plate. [13][14][15] Different concentration of synthesized ZnO nanoparticles in dimethyl sulfoxide (DMSO): Methanol (1:1V/V) (Merck, Germany) was prepared. Wells in 6 mm diameters were punctured in the media using sterile cork borers and were filled with 20 μL of the ZnO nanoparticles suspension.…”

Section: Assay For Antibacterial Activity Of Zno Nanoparticlesmentioning

confidence: 99%

In Vitro Evaluation of Antibacterial Properties of Zinc Oxide Nanoparticles on Pathogenic Prokaryotes

Nazoori

Kariminik

2018

J Apple Biotechnol Rep

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Introduction: Recent increases in microbial resistance to multiple antibiotics have led to the emergence of more economical methods for producing nanoparticles with physical, chemical effects and limited resistance. The aim of this research was to study zinc oxide (ZnO) nanoparticles synthesis and antibacterial properties against some gram-negative and gram-positive bacteria. Material and Methods: In this study, ZnO nanoparticle was synthesized using ultrasonic method and bioassayed on 10 human pathogenic bacteria by agar well diffusion method. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as well. Antibiotic resistance pattern of bacteria was determined to 9 antibiotics: gentamicin, ampicillin, nalidixic acid, amoxicillin, amikacin, ciprofloxacin, co-trimoxazole, norfloxacin and cephalexin by disc diffusion assay. Results: The nanoparticles were synthesized with suitable morphology and distribution. All gram-positive and gram-negative bacteria were inhibited at the low concentration of ZnO nanoparticles most bacteria had resistance to antibiotics. Conclusions: The findings suggest that the ZnO nanoparticles have potential applications as antibacterial compounds and their mechanism of action is dependent upon composition and surface modifications.

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Optimization of purification procedure for horse F(ab´)2 antivenom against <i>Androctonus crassicauda</i> (Scorpion) venom

Cited by 20 publications

References 10 publications

Effect of Adipose-Derived Mesenchymal Stem Cell Repair in Nicotine Model Rat: A Femoral Bone Diaphyseal Defect Study

Effect of Adipose-Derived Mesenchymal Stem Cell Repair in Nicotine Model Rat: A Femoral Bone Diaphyseal Defect Study

Evaluation of the repair of diaphyseal fracture of femoral bone using bone marrow mesenchymal stem cells in nicotine-bearing rat

In Vitro Evaluation of Antibacterial Properties of Zinc Oxide Nanoparticles on Pathogenic Prokaryotes

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