In Vitro Evaluation of Antibacterial Properties of Zinc Oxide Nanoparticles on Pathogenic Prokaryotes

Abstract

Introduction: Recent increases in microbial resistance to multiple antibiotics have led to the emergence of more economical methods for producing nanoparticles with physical, chemical effects and limited resistance. The aim of this research was to study zinc oxide (ZnO) nanoparticles synthesis and antibacterial properties against some gram-negative and gram-positive bacteria. Material and Methods: In this study, ZnO nanoparticle was synthesized using ultrasonic method and bioassayed on 10 human pathogenic bacteria by agar well diffusion method. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as well. Antibiotic resistance pattern of bacteria was determined to 9 antibiotics: gentamicin, ampicillin, nalidixic acid, amoxicillin, amikacin, ciprofloxacin, co-trimoxazole, norfloxacin and cephalexin by disc diffusion assay. Results: The nanoparticles were synthesized with suitable morphology and distribution. All gram-positive and gram-negative bacteria were inhibited at the low concentration of ZnO nanoparticles most bacteria had resistance to antibiotics. Conclusions: The findings suggest that the ZnO nanoparticles have potential applications as antibacterial compounds and their mechanism of action is dependent upon composition and surface modifications.

Green Synthesis of Zinc Oxide Nanoparticles from Pomegranate (Punica granatum) Extracts and Characterization of Their Antibacterial Activity

“…Ibrahem et al [ 55 ] also recounted the concentration-dependent manner of zinc oxide nanoparticles. Nazoori and Kariminik [ 56 ] investigated the antimicrobial efficiency of zinc oxide nanoparticles against ten (10) human pathogenic organisms, and reported that the minimum concentration of zinc oxide that can inhibit or prevent the growth of most of S. aureus , S. marcescens and E. coli cultures was 2.5mg/mL of synthesized ZnO-NPs, while the minimum concentration required to kill off the bacterial strains was 10mg/mL. In another study by Akbar et al [ 33 ], ZnO nanoparticles were synthesized and their antimicrobial potential against foodborne pathogens S. typhimurium and S. aureus was evaluated.…”

Preparation and of PVA-based compositions with embedded silver, copper and zinc oxide nanoparticles and assessment of their antibacterial properties

“…However, authors claim that the price of zinc oxide is an encouraging factor in applying it in anti-microbial products. Iranian researchers [ 68 ] have documented the anti-microbial activity of zinc oxide nanoparticles against ten human pathogens, including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, Salmonella typhi, Acinetobacter baumannii, Citrobacter freundii, Proteus mirabilis, Staphylococcus aureus and Bacillus cereus . The bioassay was based on the agar well diffusion method.…”

Synergistic effect of Zinc Oxide nanoparticles and Vancomycin on Methicillin resistant Staphylococcus aureus

“…Measurement of the minimum inhibitory concentration of vancomycin and zinc nanoparticles MIC results of the current study contradicted the results of several studies, including the study of the researcher Karim (2015), where the MIC values were (2500-325) μg/mL for ZnO 20 and ZnO 150-50 and nm 30 and the MIC values were between (2600-126.5) μg/mL Whereas in Abdulrahman and Nssaif (2016) the MIC values for ZnO 20 were (256) μg/mL, the MIC for ZnO 30 was (341) μg/mL and ZnO 150-50 was (160) μg/mL. MIC results in the study of Nazoori and Kariminik (2018) were ZnO (2500 -5000) μg /mL but for other bacterial species. In the study of Aleaghil et al (2016), the MIC values of ZnO ranged between (5000-625) μg/mL, which was close to the results of the current study.…”