Optimization of process parameters for fabrication of electrospun nanofibers containing neomycin sulfate and Malva sylvestris extract for a better diabetic wound healing

Abstract

Diabetes mellitus is one of the most concerning conditions, and its chronic consequences are almost always accompanied by infection, oxidative stress, and inflammation. Reducing excessive reactive oxygen species and the wound’s inflammatory response is a necessary treatment during the acute inflammatory phase of diabetic wound healing. Malva sylvestris extract (MS) containing nanofibers containing neomycin sulfate (NS) were synthesized for this investigation, and their impact on the healing process of diabetic wounds was assessed. Using Design Expert, the electrospinning process for the fabrication of NS nanofibers (NS-NF) was adjusted for applied voltage ( X 1 ), the distance between the needle’s tip and the collector ( X 2 ), and the feed rate ( X 3 ) for attaining desired entrapment efficacy [EE] and average nanofiber diameter (ND). The optimal formulation can be prepared with 19.11 kV of voltage, 20 cm of distance, and a flow rate of 0.502 mL/h utilizing the desirability approach. All the selected parameters and responses have their impact on drug delivery from nanofibers. In addition, M. sylvestris extracts have been added into the optimal formulation [MS-NS-NF] and assessed for their surface morphology, tensile strength, water absorption potential, and in vitro drug release studies. The NS and MS delivery from MS-NS-NF has been extended for more than 60 h. M. sylvestris -loaded nanofibers demonstrated superior antibacterial activity compared to plain NS nanofibers. The scaffolds featured a broad aspect and a highly linked porous fibrous network structure. Histomorphometry study and the in vitro scratch assay demonstrate the formulation’s efficacy in treating diabetic wound healing. The cells treated with MS-NS-NF in vivo demonstrated that wound dressings successfully reduced both acute and chronic inflammations. To improve the healing of diabetic wounds, MS-NS-NF may be regarded as an appropriate candidate for wound dressing.

A Sequential Electrospinning of a Coaxial and Blending Process for Creating Double-Layer Hybrid Films to Sense Glucose

“…Electrospinning is a versatile technique that can be used to fabricate functional nanomaterials through the encapsulation of functional ingredients [ 75 , 76 , 77 , 78 , 79 ]. The morphology of the resulting nanofibers is largely determined by the filament-forming polymeric matrix [ 80 , 81 ]. Here, the host polymers PVP and EL100 have fine electrospinnability.…”

“…By HSL conversion of the response color of 1–15 mmol/L, the color phase angle of each color was obtained, and its angle value was linearly fit, yielding R 2 = 0.96707 ( Figure 9 c), which already includes the human blood glucose concentration range (3.9–6.1 mmol/L), meeting the needs for simple testing of human blood glucose. Today, new kinds of polymers [ 80 , 81 , 89 ], organic and inorganic nanoparticles [ 90 , 91 ], and novel strategies for biomedical applications [ 92 , 93 , 94 , 95 , 96 , 97 ] are continuously reported. These achievements can be borrowed into the design and development of electrospun nanofibers, and thus promote new kinds of sensors and biomedical devices.…”

Use of customized 3-dimensional printed mandibular prostheses with a dental implant pressure-reducing device in mandibular body defect: A finite element study performing multiresponse surface methodology

“…In the DesignXplorer environment, we generated samples by using the Latin hypercube sampling method, which effectively prevents repetitive sampling and requires 20%–40% fewer simulation loops than does the direct Monte Carlo simulation technique. 32 , 33 We analyzed 25 samples to examine response surface construction ( Table 5 ).…”

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

“…The distal tissue ischemia and hypoxia may even increase the risk of ulceration or gangrene. 20,119 Consequently, the stimulation of angiogenesis was essential for the treatment of wounds in diabetic patients. 120 Curcumin was incorporated into the mixture and used to create nanofibrous materials through electrostatic spinning technologies for the purpose of treating full-thickness skin wounds on the dorsal area of diabetic rats.…”