Optimization of precision and throughput in micellar elektrokinetic chromatography

Hayashi, Yuzuru; Matsuda, Rieko; Terabe, Shigeru

doi:10.1007/bf02275852

Cited by 9 publications

(2 citation statements)

References 13 publications

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“…The simultaneous optimization of precision and analysis time has been discussed in [433] (cf: Section 2.4). Response factors can be determined by electrophoresis of standard solutions of known concentration.…”

Section: Miscellaneousmentioning

confidence: 99%

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Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

1998

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This review is in support of the development of selective, reproducible and validated capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological applications, the successful use of CE is demonstrated by more than 800 references, mainly from 1994 until 1998. Approximately 80 recent reviews have been catalogued. These articles sum up the existing strategies for method development in CE, especially in the search for generally accepted concepts, but also looking for new, promising reagents and ideas. General strategies for method development were derived not only with regard to selectivity and efficiency, but also with regard to precision, short analysis time, limit of detection, sample pretreatment requirements and validation. Standard buffer recipes, surfactants used in micellar electrokinetic capillary chromatography (MEKC), chiral selectors, useful buffer additives, polymeric separation media, electroosmotic flow (EOF) modifiers, dynamic and permanent coatings, actions to deal with complex matrices and aspects of validation are collected in 20 tables. Detailed schemes for the development of MEKC methods and chiral separations, for optimizing separation efficiency, means of troubleshooting, and other important information for key decisions during method development are given in 19 diagrams. Method development for peptide and protein separations, possibilities to influence the EOF and how to stabilize it, as well as indirect detection are considered in special sections.

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Section: Miscellaneousmentioning

confidence: 99%

“…by increasing the EOF (demonstrated in [432]), or by inducing an additional flow using a pump, if this does not impair the separation. The simultaneous optimization of precision and analysis time is discussed in [433]. Parameters such as buffer composition and temperature have a major influence on the EOF and thus on the analysis time.…”

Section: Analysis Timementioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

1998

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Multivariate optimization in micellar electrokinetic capillary chromatography

et al. 2000

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Optimized separation of seven corticosteroids by micellar electrokinetic chromatography

et al. 1994

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The corticosteroids studied can be effectively separated by employing micellar electrokinetic capillary chromatography (MECC). The effect of pH, borate concentration and the sodium dodecyl sulfate (SDS) concentration on both the resolution and the selectivity was studied under 15 different experimental conditions. The experimental design was similar to the central composite design approach. Empirical quadratic regression models were derived for analyte migration time, band broadening and analyte velocity. Satisfactory regression fits and coefficients of determination for prediction were obtained with cross-validated models. The models for analyte migration time and analyte velocity were in good agreement with theory. Modeling of the band broadening seemed to be somewhat more complicated. Optimum conditions for resolution and selectivity were different. This is due to the fact that selectivity studies ignore the electroosmotic and band broadening properties of different electrolyte solutions. However, the study of the selectivity yielded good information about the suitability of the electrolyte systems for the particular separation problem. Although a high solubilizing power of SDS caused the corticosteroids to partition strongly into the SDS micelles, a good separation could be achieved at low SDS concentrations.

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Optimization of precision and throughput in micellar elektrokinetic chromatography

Cited by 9 publications

References 13 publications

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

Multivariate optimization in micellar electrokinetic capillary chromatography

Optimized separation of seven corticosteroids by micellar electrokinetic chromatography

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