Multivariate optimization in micellar electrokinetic capillary chromatography

Lee, Ruijiang; Liu, Zhen; Zou, Hanfa; Ni, Jincheng; Zhang, Yukui

doi:10.1002/1520-667x(2000)12:6<356::aid-mcs4>3.0.co;2-g

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…An iterative optimization method with step-length searching and iterative optimization previously developed [22] was also used in optimizing the separation condition of alkaloids. Table 5 shows the results with two optimization methods.…”

Section: Resultsmentioning

confidence: 99%

Application of uniform design and genetic algorithm in optimization of reversed-phase chromatographic separation

Chen¹,

Li²,

Kong³

et al. 2003

Chemometrics and Intelligent Laboratory Systems

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Section: Resultsmentioning

confidence: 99%

Application of uniform design and genetic algorithm in optimization of reversed-phase chromatographic separation

Chen¹,

Li²,

Kong³

et al. 2003

Chemometrics and Intelligent Laboratory Systems

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“…Generally, an initial group includes 20 to 300 points. In this work, an initial group containing 30 two-dimensional points was generated with the uniform design method [9] in the region of optimization parameters.…”

Section: A Initializationmentioning

confidence: 99%

Application of Uniform Design Genetic Algorithm in Separation Condition Optimization of Ginkgo Biloba Extract

Chen¹

2015

Proceedings of the 2015 International Conference on Mechatronics, Electronic, Industrial and Control Engineering

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Abstract-Objective: aiming to establish a new strategy based on uniform design and genetic algorithm and applying in the optimization of the separation condition of Ginkgo biloba extract using reversed-phase liquid chromatography according to traditional optimization methods. Methods: based on uniform design and genetic algorithm, a new strategy was developed and a software package was developed. The optimization strategy was applied in the optimization of separation condition of Ginkgo biloba extract in reversed-phase chromatography, the volume fraction of acetonitrile and rate of linear gradient-elution were selected as the optimization variables. Results: The optimization of separation condition of Ginkgo biloba extract in reversed-phase chromatography was performed and satisfactory optimal result was obtained as the initial concentration of acetonitrile was 14.4% and the rate of linear gradient-elution was 0.22%, respectively. Conclusion: The results shown that the method using uniform design and genetic algorithm is efficient and it has potential application not only in the optimization of two parameters, but also of much more than two parameters.

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Separation and analysis of cis-diol-containing compounds by boronate affinity-assisted micellar electrokinetic chromatography

Wang

Lü

et al. 2013

Anal Bioanal Chem

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Cis-diol-containing compounds (CDCCs) are usually highly hydrophilic compounds and are therefore difficult to separate by conventional reversed-phase-based micellar electrokinetic chromatography (MEKC) due to poor selectivity. Here, we report a new method, called boronate affinity-assisted micellar electrokinetic chromatography (BAA-MEKC), to solve this issue. A boronic acid with a hydrophobic alkyl chain was added to the background electrolyte, which acted as a modifier to adjust the selectivity. CDCCs can covalently react with the boronic acid to form negatively charged surfactant-like complexes, which can partition into micelles formed with a cationic surfactant. Thus, CDCCs can be separated according to the differential partition constants of their boronic acid complexes between the micellar phase and the surrounding aqueous phase. To verify this method, eight nucleosides were employed as the test compounds and their separation confirmed that the combination of boronate affinity interaction with MEKC can effectively enhance the separation of CDCCs. The effects of experimental conditions on the separation were investigated. Finally, the BAA-MEKC method was applied to the separation and analysis of nucleosides extracted from human urine. BAA-MEKC exhibited better selectivity and improved separation as compared with conventional MEKC and CZE. Successful quantitative analysis of urinary nucleosides by BAA-MEKC was demonstrated.

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Multivariate optimization in micellar electrokinetic capillary chromatography

Cited by 4 publications

References 21 publications

Application of uniform design and genetic algorithm in optimization of reversed-phase chromatographic separation

Application of uniform design and genetic algorithm in optimization of reversed-phase chromatographic separation

Application of Uniform Design Genetic Algorithm in Separation Condition Optimization of Ginkgo Biloba Extract

Separation and analysis of cis-diol-containing compounds by boronate affinity-assisted micellar electrokinetic chromatography

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