Optimization of polysaccharides extraction from Tricholoma mongolicum Imai and their antioxidant and antiproliferative activities

Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

“…Therefore, in this study, pectinase was used at 15-25 g/kg, and papain and cellulase were used at 10-20 g/kg in the subsequent orthogonal experiments.3.1.2 Effect of pH on polysaccharide extraction yieldThe pH value can significantly affect the yields of the target components in the used to evaluate the influence of pH on the LEP extraction when the other conditions were fixed (cellulase and papain amounts of 15 g/kg, pectinase amount of 20 g/kg, temperature 40°C, enzymatic treatment time 70 min and liquid/material ratio 20:1 mL/g).Fig.1bshows the effect of pH on the LEP extraction yield. The yield of LEPs increased with the increasing pH and reached a maximum at pH 5.0, which is in agreement with Wang et al and Wu et al[16] …”

“…The crude LEPs were purified by DEAE-52 anion-exchange chromatography and 6 Sephadex G-100 chromatography according to previously reported methods [15,16].…”

Optimization of cellulase‐assisted extraction process and antioxidant activities of polysaccharides from Tricholoma mongolicum Imai

“…As a result, each section gave a single elution peak. The results in this study were also similar to the findings of Wang et al 8 in the extraction of TMIPs.…”

“…Therefore polysaccharides together with proteins, lipids and polynucleotides constitute the four principal biological macromolecules in life science. In addition, polysaccharides extracted from natural products have already been proved to possess various potential pharmacological effects such as antioxidant, antitumor, immunological, antiproliferative and hepatoprotective effects. Because of the non‐toxic, biocompatible and biodegradable properties of polysaccharides, considerable attention has been focused on the extraction of bioactive polysaccharides from natural products for novel and highly value‐added applications such as pharmaceutical biomedical and cosmetic applications.…”

Optimization of ultrasonic‐microwave synergistic extraction of polysaccharides from Morchella conica

“…From the results, the higher model F‐ values (182.3) demonstrated that the developed model was significant (Hifney, Fawzy, Abdel‐Gawad, & Gomaa, ). Moreover, a lower p value ( p < .0001) implied the corresponding model term was highly significant (Wang, Zhao, Wang, & Shen, ). The high R 2 (.9958), adj‐ R 2 (.9903), and pre‐ R 2 (.9672) values also demonstrated a close agreement between the response and independent variables predicted by polynomial model.…”