Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

Zhao, Yijie; Jin, Wang; Wu, Zhigang; Yang, Jianming; Li, Wei; Li-xia, Shen

doi:10.1016/j.ijbiomac.2016.05.100

Cited by 78 publications

(31 citation statements)

References 34 publications

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“…As shown in Fig. 1c, the HEP yield increased with elevated pH levels and reached a critical value (13.3%) at pH 5.0 which was in agreement with previous works (Wang et al, 2015;Zhao et al, 2016). However, further increase in pH resulted in decreasing HEP yield, which may be due to the decrease of enzyme activities at higher pH value (Wang et al, 2013).…”

Section: Effect Of Different Ph On Extraction Yield Of Polysaccharidessupporting

confidence: 92%

Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from <i>Hericium erinaceus</i> mycelia

Qin

Zhang

Song

et al. 2017

FSTR

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In this study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from Hericium erinaceus mycelia (HEP) using the Box-Behnken design based on the results of single factor experiments. As a result, the optimal protocol was as follows: an extraction time of 79 min, an extraction temperature of 50℃, a pH of 5.7 and a ratio of water to raw materials of 33.4 mL/g. These optimal extraction conditions achieved the highest yield (13.9 ± 0.3%) of HEP. The purified fraction (HEP-2) was composed of glucose, galactose, mannose, fucose, arabinose, xylose and rhammnose. HEP-2 manifested an obvious inhibitory effect on the growth of HeLa cells. The overall findings implied that enzyme-assisted procedure is a superior method and that HEP-2 might be useful for developing natural and safe antitumor drugs.

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Section: Effect Of Different Ph On Extraction Yield Of Polysaccharidessupporting

confidence: 92%

Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from <i>Hericium erinaceus</i> mycelia

Qin

Zhang

Song

et al. 2017

FSTR

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“…The significance of parameters and the interaction of each coefficient were checked by F-test and P-value. Meanwhile, the precision and validity of the model were evaluated by the values of determination coefficient (R 2 ) and adjusted determination coefficient (R 2 adj) (Zhao et al, 2016). Table 3 listed the variance analysis (ANOVA) for the response surface quadratic model of WLP extraction.…”

Section: Optimization Of the Extraction Process By Rsm Models Fittingmentioning

confidence: 99%

Optimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in Vitro

Yang¹,

Xiao²,

Yu³

et al. 2019

American Journal of Biochemistry and Biotechnology

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Cellulase-assisted extraction of Walnut Leaf Polysaccharide (WLP) was studied. Single factor test and response surface design were employed to optimize the technological conditions. The consequences indicated that the highest WLP yield of 5.21% was achieved with raw material ratio of 50 mL/g, cellulase dose of 41488.52 U/g, enzymolysis pH of 5.88, enzymolysis time of 29.74 min and enzymolysis temperature of 52.07°C. Moreover, in vitro antioxidant assays revealed that WLP has significant eliminating capability against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl free radicals in a dose-dependent manner and exhibited a strong reducing power. These results suggest that WLP could be developed as a promising natural antioxidant agent in the pharmaceutical and functional food industries.

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“…Polysaccharides are important biological macromolecules and have attracted considerable attention due to their biocompatibility, low toxicity, unique physical properties, and specific therapeutic properties [ 1 ]. Many natural polysaccharides derived from plants possess proven biological properties, including antitumor, antioxidant, antibacterial, anti-inflammatory, and immunoregulatory activities [ 2 , 3 , 4 , 5 ]. Immunostimulation in particular is one of the most important actions of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, several new and innovative methods (e.g., enzyme-assisted extraction, microwave-assisted extraction, and ultrasound-assisted extraction) have extracted polysaccharides from natural resources [ 21 ]. Enzyme-assisted extraction is considered a mild, efficient, and environmentally friendly method, with several advantages, such as low energy consumption and easy operation standards [ 3 ]. The assistance of enzymes, which have been employed frequently as biocatalysts to obtain target compounds from various plants, can improve the extraction efficiency, yield, and/or biological activities of polysaccharides [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber

Song

Sung

Shin

et al. 2018

IJMS

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The edible and medicinal perennial herb Aster scaber is known to have anticancer, antioxidant, and immunomodulatory properties. However, the biological effects of its polysaccharides are not well understood. Here, we aimed to extract novel polysaccharides with enhanced biological properties from Aster scaber using enzyme-assisted methods. Amylase, cellulase, and pectinase were used to extract enzyme-assisted polysaccharide (ASEP)-A, ASEP-C, and ASEP-P, respectively. The yields, physicochemical properties, and immunostimulatory activities of the polysaccharides were investigated and compared with those of hot water extracted polysaccharide (ASWP). The highest yield (3.8%) was achieved for ASEP-P extracted using pectinase digestion. Fourier-transform infrared spectroscopy (FT-IR) and chemical composition analysis revealed that ASWP and three ASEPs were typical acidic heteropolysaccharides, mainly comprising rhamnose, arabinose, galactose, glucose, and galacturonic acid. Immunostimulatory activity assays on RAW264.7 macrophages showed ASEP-P to have the greatest immunostimulatory potential in terms of nitric oxide (NO) and cytokine productions and phagocytic activity. ASEP-P administration improved immune-enhancing effects in normal mice by improving the spleen index and splenic lymphocyte proliferation, and in immunosuppressed mice by modulating lymphocyte proliferation, natural killer (NK) cell activity, and leukocyte counts. The ASEP-P derived from pectinase hydrolysate of Aster scaber demonstrated efficacious immunostimulatory properties and has potential applications as an immune stimulator.

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Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

Cited by 78 publications

References 34 publications

Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from <i>Hericium erinaceus</i> mycelia

Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from <i>Hericium erinaceus</i> mycelia

Optimization for the Extraction of Polysaccharide from Walnut (Juglans regia L.) Leaves: Antioxidant Activities in Vitro

The Effect of Pectinase-Assisted Extraction on the Physicochemical and Biological Properties of Polysaccharides from Aster scaber

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