Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from <i>Hericium erinaceus</i> mycelia

Abstract

In this study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from Hericium erinaceus mycelia (HEP) using the Box-Behnken design based on the results of single factor experiments. As a result, the optimal protocol was as follows: an extraction time of 79 min, an extraction temperature of 50℃, a pH of 5.7 and a ratio of water to raw materials of 33.4 mL/g. These optimal extraction conditions achieved the highest yield (13.9 ± 0.3%) of HEP. The purified fraction (HEP-2) was composed of glucose, galactose, mannose, fucose, arabinose, xylose and rhammnose. HEP-2 manifested an obvious inhibitory effect on the growth of HeLa cells. The overall findings implied that enzyme-assisted procedure is a superior method and that HEP-2 might be useful for developing natural and safe antitumor drugs.

Optimization of Aqueous Two‐Phase Extraction of Polysaccharides from Hawthorn by Response Surface Methodology Coupled with Genetic Algorithm and Its Antioxidant Activity

“…This phenomenon may be attributed to the fact that at the initial stage of extraction, the concentration difference of HPs inside and outside the cells was large, which accelerated the dissolution and diffusion of HPs, and then improved HPs yield. [ 10 ] After extraction time 30 min, the yield of HPs showed no significant change with the prolongation of extraction time ( p > 0.05). Similar results were found in the case of polysaccharides from G. frondosa by using ATPE.…”

“…Euclidean distance is used to express the deviation of each variable from the response value, which is counted by Equation (10).…”

“…EAE can decompose plant tissue mildly through enzyme reaction, destroy plant cell wall, expand the mass transfer area of the diffusion of effective components in the cell to the extraction medium, reduce mass transfer resistance, and increase the yield of effective components. [ 10 ] However, high temperature easily leads to the inactivation and denaturation of enzymes, which cannot achieve the efficient extraction of polysaccharides. Hence, EAE must strictly control the extraction conditions.…”

Optimized Isolation and Characterization of the Major Polysaccharide from Grape Pomace

“…Grape pomace is the major component of its fruits, contributing roughly half of the total weight, and poses a severe environmental issue after winemaking and juice processing [1]. Utilization of grape pomace by bioactive components extraction has been extensively performed, and minor components such as anthocyanins, hydroxycinnamic acids, flavanols, and flavanol glycosides in grape pomace are characterized [2][3][4][5]. However, little is known about the utilization of the major macromolecular components in grape pomace.…”

Research Progress on Biological Activities of Polysaccharides from Edible Fungus

“…Dictyophora Antioxidant, immunoregulatory Scavenge hydroxyl radical, superoxide anion, and DPPH; possess reducing power; inhibit the expression of TLR4, phosphorylated-I𝜅B𝛼 and NF-𝜅B nuclear translocation (p65 subunit); decrease the extracellular protein level of pro-caspase-1 and cytoplasmic pool of NLRP3; stimulate the proliferation of immune cells and reverse inhibition of the growth of CD4+/CD8+ T cells; down-regulate the expression of 𝛼-smooth muscle actin, etc [8,79]. H. erinaceus Antioxidant, immunoregulatory, regulating gut microbiota, hypoglycemic, anti-tumor, gastroprotective activitiesInducing apoptosis of tumor cells, recognition of the receptor of the cell surface, the cell cycle arrest, and necrosis anti-angiogenesis; activate repair and defense system, decrease the inflammatory response, and alleviate the oxidative injury, etc [ 114,127,128].…”