Optimization of paper-based nanoparticle immunoassays for direct detection of the bacterial pathogen<i>V. parahaemolyticus</i>in oyster hemolymph

Rodriguez-Quijada, Cristina; Lyons, Casandra; Santamaria, Charles; Quinn, S P; Tlusty, Michael F.; Shiaris, Michael P.; Hamad‐Schifferli, Kimberly

doi:10.1039/d0ay00725k

Cited by 10 publications

(12 citation statements)

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“…The fifth route is based on NP accumulation reactions [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. This strategy has been generally used in flat substrate-based systems, such as the lateral flow assay (LFA), µ-paper-based analytical device (µ-PAD), and paper-based dipstick system [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. These systems rely on the capillary action of fluids and colored NPs.…”

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

“…The concentrated NPs act as colorimetric labels or color generation factors. Colored NPs used for colorimetric detection include AuNPs, silver NPs (AgNPs), and colloidal carbon [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. Red AuNPs are one of the widely used colorimetric labels because of their bright color, high stability, and high biocompatibility [ 80 ].…”

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

“…The fifth route is based on NP accumulation reactions [65][66][67][68][69][70][71][72][73][74][75][76][77][78][79]. This strategy has been generally used in flat substrate-based systems, such as the lateral flow assay (LFA), µ-paperbased analytical device (µ-PAD), and paper-based dipstick system [65][66][67][68][69][70][71][72][73][74][75][76][77][78][79].…”

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

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Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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Bacterial contamination is a public health concern worldwide causing enormous social and economic losses. For early diagnosis and adequate management to prevent or treat pathogen-related illnesses, extensive effort has been put into the development of pathogenic bacterial detection systems. Colorimetric sensing systems have attracted increasing attention due to their simple and single-site operation, rapid signal readout with the naked eye, ability to operate without external instruments, portability, compact design, and low cost. In this article, recent trends and advances in colorimetric systems for the detection and monitoring of bacterial contamination are reviewed. This article focuses on pathogen detection strategies and technologies based on reaction factors that affect the color change for visual readout. Reactions used in each strategy are introduced by dividing them into the following five categories: external pH change-induced pH indicator reactions, intracellular enzyme-catalyzed chromogenic reactions, enzyme-like nanoparticle (NP)-catalyzed substrate reactions, NP aggregation-based reactions, and NP accumulation-based reactions. Some recently developed colorimetric systems are introduced, and their challenges and strategies to improve the sensing performance are discussed.

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Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

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Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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“…An immunological dipstick strip was developed to detect pathogenic Vibrio parahaemolyticus directly from oyster hemolymph (oyster circulatory fluid) [ 45 ]. Antibody was electrostatically conjugated on AuNPs, followed by passivation using thiolated polyethylene glycol (PEG) to prevent non-specific interaction.…”

Section: Noble Metal Nanoparticle-mediated Colorimetric Pathogen Detementioning

confidence: 99%

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Nguyen

Kim

2020

TrAC Trends in Analytical Chemistry

146

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The pervasive spread of infectious diseases and pandemics, such as the 2019 coronavirus disease (COVID-19), are becoming increasingly serious and urgent threats to human health. Preventing the spread of such diseases prioritizes the development of sensing devices that can rapidly, selectively, and reliably detect pathogens at minimal cost. Paper-based analytical devices (PADs) are promising tools that satisfy those criteria. Numerous paper-based biosensors have been established that rival conventional pathogen detection methods. Among them, colorimetric strategies are promising since results can be interpreted by eye, and are simple to operate, which is advantageous for point-of-care testing (POCT). Particularly, the application of nanomaterials on paper-based biosensors has become important as these materials are capable of converting signals from pathogens through unique mechanisms to yield an amplified colorimetric readout. To highlight the research progress on using nanomaterials in colorimetric paper-based biosensor for pathogen detection, we discuss the sensing mechanisms of how they work, structural and analytical characteristics of the devices, and representative recent applications. Current challenges and future directions of using PADs and nanomaterial-mediated strategies are also discussed.

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“…Compared to molecular biology‐based methods, immunological detection methods did not require complex instrument, are easy to perform and have high specificity, which may be more suitable for field detection of V. parahaemolyticus . Presently, a variety of immunological detection assays have been proposed for the detection of V. parahaemolyticus , including enzyme‐linked immunosorbent assay (ELISA) (Pattipeilohy & Sundari, 2019), electrochemiluminescence (ECL) (Sha et al, 2016; Wang et al, 2019) and lateral flow immunoassay strip (LFIS) (Rodriguez‐Quijada et al, 2020; Wu et al, 2021a). Among these, LFIS have been attracting increasing attention for being rapid and easy to operate, yielding results within a few minutes.…”

Section: Introductionmentioning

confidence: 99%

Detection of trh⁺Vibrio parahaemolyticus in seafood by lac dye coloration‐based label‐free lateral flow immunoassay strip

Liu

Tian

et al. 2022

Journal of Fish Diseases

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Vibrio parahaemolyticus (V. parahaemolyticus) is an important foodborne pathogen known to cause severe enteric disease. Thus, timely detection of V. parahaemolyticus in seafood is crucial to prevent food poisoning and reduce economic losses. Traditional lateral flow immunoassay strips (LFIS) required good labelling materials and pairing of two antibodies, which made them costly and difficult to manufacture. In this study, a label‐free and lac dye coloration‐based LFIS (LD‐LFIS) to detect trh+ V. parahaemolyticus was developed for the first time. Lac dye was used to stain V. parahaemolyticus, and LFIS was used to detect stained bacteria. Dimethyl sulphoxide (DMSO) and simultaneous mordanting were chosen as the best solvent and the best staining method for lac dye. In addition, three mordants [KAl(SO4)2·12H2O, NH4Fe(SO4)2·12H2O, and AlCl3·6H2O] were selected to improve dyeing efficiency. The detection limit of LD‐LFIS was 3.9 × 105 CFU/ml when NH4Fe(SO4)2·12H2O was used as mordant. Feasibility of the LD‐LFIS method was verified by detecting trh+ V. parahaemolyticus in true and spiked seafood samples. The method developed in this study is expected to reduce restrictions on labelling materials and pairing of two antibodies on LFIS, and proposes a novel idea for the rapid detection of V. parahaemolyticus in seafood.

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Optimization of paper-based nanoparticle immunoassays for direct detection of the bacterial pathogenV. parahaemolyticusin oyster hemolymph

Abstract: The detection of foodborne pathogens is critical for disease control and infection prevention, especially in seafood consumed raw or undercooked.

Cited by 10 publications

References 44 publications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Detection of trh⁺Vibrio parahaemolyticus in seafood by lac dye coloration‐based label‐free lateral flow immunoassay strip

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Optimization of paper-based nanoparticle immunoassays for direct detection of the bacterial pathogenV. parahaemolyticusin oyster hemolymph

Abstract: The detection of foodborne pathogens is critical for disease control and infection prevention, especially in seafood consumed raw or undercooked.

Cited by 10 publications

References 44 publications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Detection of trh+Vibrio parahaemolyticus in seafood by lac dye coloration‐based label‐free lateral flow immunoassay strip

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Product

Resources

About

Detection of trh⁺Vibrio parahaemolyticus in seafood by lac dye coloration‐based label‐free lateral flow immunoassay strip