Optimization of PAMAM-gold nanoparticle conjugation for gene therapy

Figueroa, Elizabeth R.; Lin, Adam Yuh; Yan, J. Stephen; Luo, Laureen; Foster, Aaron E.; Drezek, Rebekah A.

doi:10.1016/j.biomaterials.2013.11.026

Cited by 78 publications

(59 citation statements)

References 36 publications

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“…Cationic water-stable carbosilane dendrimers containing ammonium groups at the periphery are reported to be nontoxic for gene therapy against HIV and hepatocarcinoma (de Las Cuevas et al 2012). AuPAMAM conjugates are reported to exhibit high yield, high transfection efficiency, and low cytotoxicity (Figueroa et al 2014). …”

Section: Cationic Dendrimersmentioning

confidence: 99%

Cationic Polymers for the Delivery of Therapeutic Nucleotides

et al. 2015

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Section: Cationic Dendrimersmentioning

confidence: 99%

Cationic Polymers for the Delivery of Therapeutic Nucleotides

et al. 2015

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“…172 In another report, cationic gold nanoparticles prepared by NaBH 4 reduction in the presence of 2aminoethanethiol formed a complex structure with plasmid DNA expressing a luciferase gene. 171 173 Figueroa et al 174 conjugated gold nanoparticles (AuNP) to polyamidoamine ( Fig. 5).…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Synthetic <I>In Vitro</I> Delivery Systems for Plasmid DNA in Eukaryotes

Avila¹,

Lee²,

Tomich³

2014

Journal of Nanopharmaceutics and Drug Delivery

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Success for gene therapy clinical protocols depends on the design of safe and efficient gene carriers. Nature had already designed efficient DNA or RNA delivery devices, namely virus particles. However, they have a propensity to trigger neutralizing and other immune responses and insertional mutagenesis have limited their clinical use. Alternatively, safer approaches involving non-viral carriers have been and continue to be developed although they have not reached the transfection levels achieved by viruses. Those methods can be broadly classified into two categories: chemical and physical methods. In this review we present the most common and recent chemical non-viral methods to introduce, in vitro, pDNA into eukaryotic cells.

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“…It is pertinent to mention that several researchers used modified PAMAM dendrimers and successfully transfected therapeutic gene in vitro . However, the efficiency of these modified/synthesized dendrimers was low and nonspecific in in vivo …”

Section: Introductionmentioning

confidence: 99%

Role of Unmodified Low Generation - PAMAM Dendrimers in Efficient Non-Toxic Gene Transfection

Alajangi¹,

Natarajan

Vij

et al. 2016

ChemistrySelect

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The present study is focused on a simple execution in the applicability of non-toxic lower generation poly amidoamine (PA-MAM) dendrimers as effective nano-vectors in targeted gene delivery to the skin. So far the first three lower generation (G1, G2 and G3) PAMAM dendrimers have been overlooked as nucleic acid therapeutics. In this study, we have first carried out a systematic biophysical analysis to analyze their ability for plasmid DNA (pDNA) condensation and subsequent release by ethidium bromide assay to optimize dendrimer to DNA charge ratio for effective pDNA condensation and release. Interestingly, stopped flow fluorescence spectroscopic analysis on pDNAdendrimer binding kinetics revealed the efficiency of generations G2 and G3 in pDNA condensation in comparison with G1 through four steps. Importantly, it validates aggregation and association of the dendrimer in the vicinity of pDNA. Based on these understanding, successful in vitro cellular uptake of dendriplexes followed by their transfection into CHOÀK1 cells was demonstrated at the charge ratio Z + /-5 and 10. The interesting observations of gene transfection with CHOÀK1 cells were extended to HaCaT cell line and efficient pDNA transfections were evidenced with negligible cytotoxicity. In addition to this, the stability of the dendriplexes at the charge ratio Z + /-5 for G3 and Z + /-10 for G2 was found even upto 50 % serum concentration suggesting possible future applicability in in vivo. Overall, the current approach promises the use of lower generation dendrimers for pDNA delivery to the skin using only electrostatic nanocomplex formation without any covalent linkage.

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Optimization of PAMAM-gold nanoparticle conjugation for gene therapy

Cited by 78 publications

References 36 publications

Cationic Polymers for the Delivery of Therapeutic Nucleotides

Cationic Polymers for the Delivery of Therapeutic Nucleotides

Synthetic <I>In Vitro</I> Delivery Systems for Plasmid DNA in Eukaryotes

Role of Unmodified Low Generation - PAMAM Dendrimers in Efficient Non-Toxic Gene Transfection

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