Cationic Polymers for the Delivery of Therapeutic Nucleotides

Jain, Anjali; Hosseinkhani, Hossein; Domb, Abraham J.; Khan, Wahid

doi:10.1007/978-3-319-16298-0_44

Cited by 13 publications

(9 citation statements)

References 126 publications

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“…Another delivery carrier is dendrimer, known for its highly branched structure, uniform dispersion and nano size range T (Uppuluri et al, 1998). For gene delivery application, Polyamidoamine (PAMAM) dendrimers are explored widely due to their narrow molecular weight distribution and easily functionalized amine-terminated surface providing the positive charge for interaction with siRNA (Kang et al, 2005;Jain et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Dendrimer as a new potential carrier for topical delivery of siRNA: A comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA

Pandi

Jain

Kommineni

et al. 2018

International Journal of Pharmaceutics

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Topical delivery of siRNA is challenging task due to complex barrier property of stratum corneum and cationic lipid based carriers have been widely explored for this purpose due to improved permeation through skin. For gene delivery application, dendrimers are considered as efficient carrier due to their cationic nature and well-defined surface groups. However, they are not well explored for topical delivery. This work compares the suitability of PAMAM dendrimer with DOTAP liposome for topical delivery of siRNA against TNF-α. The particle size, zeta potential and entrapment efficiency of dendriplex were 99.80 ± 1.80 nm, 13.40 ± 4.84 mV and 98.72 ± 2.02% whereas for lipoplex were 174.80 ± 0.80 nm, 29.96 ± 0.51 mV and 94.99 ± 5.01% respectively. Both the formulations were stable in serum and in the presence of RNAse. TNF-α is inflammatory cytokine, hence the in vivo efficacy of developed formulations was determined using psoriatic plaque model. Results suggested improved phenotypic and histopathological features and reduced levels of IL-6, TNF-α, IL-17 and IL-22 for dendriplex and lipoplex treated groups in comparison to Imiquimod treated group. These findings suggest that dendrimer can be a potential carrier for topical gene delivery.

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Section: Introductionmentioning

confidence: 99%

Dendrimer as a new potential carrier for topical delivery of siRNA: A comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA

Pandi

Jain

Kommineni

et al. 2018

International Journal of Pharmaceutics

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“…Because there are connections between the toxicity and the structures of biomaterials that mediate the effectivity of the in vivo experiments researchers should focus more on appropriate modifications that could decrease the overall cytotoxicity. Rapid development of nanotechnology will open new avenues in medical technology . Biodegradable polysaccharides have been widely used in many different medical applications, including cancer therapy, gene therapy, imaging technology, and tissue engineering .…”

Section: Resultsmentioning

confidence: 99%

Overview on natural hydrophilic polysaccharide polymers in drug delivery

Abedini

Ebrahimi

Roozbehani

et al. 2018

Polymers for Advanced Techs

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124

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Nanotechnology is poised to make potentially revolutionary innovations in areas of biomedical science, such as gene therapy and drug therapy. A recently developed nanodelivery strategy involves the use of hydrophilic polymers as carriers of proteins and siRNA. By controlling the reaction conditions during polymer production, various degrees of anionic charge, cationic charge, and cross-linking can be added, thereby changing their capabilities as protein and nucleic acid carriers and promoting effective cell membrane permeation. The efficiency of a specific controlled-release polymeric system is determined in part by its unique physical and chemical properties and biodegradation rate. In this review, we will summarize recent progress in the ability to modify drug release of hydrophilic polymers nanoparticles.

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“…More than 300 different polycations have been prepared, starting from various polysaccharides of different molecular weights and oligoamines having two to four amine groups. Although most of these conjugates formed stable complexes with DNA as determined by the ethidium bromide quenching assay, only the dextran‐spermine polycations of defined molecular weights were found to be active in transfecting a wide range of cell lines in vitro . The transfection success of the dextran‐spermine conjugate was attributed to the spermine residues, which play a crucial role in cell transfection.…”

Section: Biodegradable Polymers In Sirna Delivery Technologymentioning

confidence: 99%

Biodegradable polymers in gene‐silencing technology

Hosseinkhani

Domb

2019

Polymers for Advanced Techs

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Small interfering RNAs (siRNAs) technology has shown great promise as a new class of therapeutics invention for treatment of cancer and other diseases. siRNA has been used extensively in blocking various genes and is presently being evaluated as a therapeutic for cancer and viral disease. Despite the excitement about this remarkable biological process for sequence specific gene regulation, the major limitations against the use of siRNAs‐based therapeutics are their rapid degradation by serum nuclease, poor cellular uptake, and rapid renal clearance following systemic delivery, off‐target effects, and induction of immune responses. Many researchers have tried to overcome these limitations with developing nuclease‐resistant chemically modified siRNAs and variety of synthetic and natural biodegradable lipids and polymers for siRNA delivery to enhance efficacy and safety profiles. An ideal siRNAs‐based delivery system must be clinically suitable, safe, and effective. This review discuss the recent progress of biodegradable polymers in siRNA delivery technology.

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Cationic Polymers for the Delivery of Therapeutic Nucleotides

Cited by 13 publications

References 126 publications

Dendrimer as a new potential carrier for topical delivery of siRNA: A comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA

Dendrimer as a new potential carrier for topical delivery of siRNA: A comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA

Overview on natural hydrophilic polysaccharide polymers in drug delivery

Biodegradable polymers in gene‐silencing technology

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