Optimization of nutritional and physical parameters for enhancing the keratinase activity of Bacillus cereus isolated from soil of poultry dump site in Gurugram, Haryana

Response surface methodology based optimization of keratinase from Bacillus velezensis strain ZBE1 and nanoparticle synthesis, biological and molecular characterization

“…Researchers have reported different keratinolytic bacterial cultures isolated from diverse sources, such as Bacillus sp. NDS-10 from soil ( Akram et al, 2021 ), Pseudomonas aeruginosa 4–3 from slaughterhouses ( Pei et al, 2023 ), Bacillus cereus from poultry dump soil ( Yadav et al, 2022 ), Bacillus thuringiensis strain MT1 from cattle-yard ( Hassan et al, 2020 ), Bacillus pumilus AR57 from the slaughterhouse soil ( Jagadeesan et al, 2020 ) etc. Bacillus sps are highlighted for their display of considerable power in the bioremediation aspects of protein enriched keratinous waste which is usually considered as recalcitrant.…”

“…Keratin azure was utilized as the substrate for keratinase assay following the protocol explained by Yadav et al, (2022) . The reaction constituted of 900 μl keratin azure prepared by suspending 4 mg in 50 mM Tris–HCl buffer pH 8.0 and 100 μl enzyme (supernatant).…”