Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice

Abstract

Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM; however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential.

Clinical efficacy and safety of acellular dermal matrix as a method of volume replacement after breast-conserving surgery

“…Qing-Qing Qiu et al [13] reported that E-beam irradiation sterilization cuts the collagen matrix of the ADM. Other studies also report that the gamma and E-beam irradiation used for sterilization accelerate the rate of collagen ber degradation and fragmentation in the ADM [14,15]. The increased rate of degradation and fragmentation compromises the tensile strength of the ADM [14,15]. Since most patients who have undergone BCS surgery undergo radiation therapy, it is accepted that some shrinkage of the ADM will occur.…”

The effect of source animal age, decellularization protocol, and sterilization method on bovine acellular dermal matrix as a scaffold for wound healing and skin regeneration

“…Strong permeability, no residual toxic agents, and ambient processing temperature are advantages of the method. However, physical, chemical, mechanical, and biological changes bring some troubles 66 . EO reacts with sulfhydryl, amino, and carboxyl groups in proteins and nucleic acid molecules within the microorganisms, leading to their inactivation 67 .…”

“…However, physical, chemical, mechanical, and biological changes bring some troubles. 66 EO reacts with sulfhydryl, amino, and carboxyl groups in proteins and nucleic acid molecules within the microorganisms, leading to their inactivation. 67 Sterilization with EO gas is also conducted at room temperature.…”

Supercriticality, Glassy Dynamics, and the New Insight into Melting/Freezing Discontinuous Transition in Linseed Oil

“…These opinions are shared by doctors and nutritionists also nowadays [4,5]. Generally, the pro-health properties of linseed oil are explained by specific features of their components [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]. This report shows that yet another factor, omitted so far, can be significant: supercriticality [24][25][26][27][28].…”

“…The positive influence of linseed oil is broadly recognized in the fight against obesity, arthritis, and hypertension [9,13]. Focusing on cosmetics-related issues, it can strengthen skin, support redness reductions, repair skin damage (including flaky epidermis), heal skin burns and frostbite, and create smooth and soft skin [5,9,[17][18][19]22]. Linseed is also indicated as a prophylactic anti-cancer agent for breast, colon, and prostate problems [4,17,19,23].…”