Optimization of Non-Nutritional Factors for a Cost-Effective Enhancement of Nisin Production Using Orthogonal Array Method

Abstract

In order to increase nisin production in a cost-effective manner, non-nutritional factors as well as nutritional parameters must be optimized. In this study, optimization of the most important non-nutritional factors for nisin production using orthogonal array method was performed. Optimization of temperature, agitation, age and size of inoculum, medium initial pH value and flask volume/medium volume ratio in de Man, Rogosa and Sharpe (MRS) medium in batch fermentation was accomplished. Nisin was produced by Lactococcus lactis subsp. lactis PTCC 1336 and measured by bioassay method using Micrococcus luteus PTCC 1169 as the nisin-sensitive strain. The optimum levels of non-nutritional factors for maximum nisin production and productivity were obtained as: flask volume/medium volume ratio: 5.00, medium initial pH value: 8.00, inoculum size: 1%, inoculum age: 24 h old (A = 1.7), agitation: 100 rpm and temperature: 27 °C. Under the optimized conditions, maximum nisin production and maximum nisin productivity were 599.70 IU/mL and 37.48 IU/mL/h, respectively.

ComX improves acid tolerance by regulating the expression of late competence proteins in Lactococcus lactis F44

“…Recently, nisin has been shown to have potential clinical use in the treatment of various diseases, such as cancer, oral diseases, and immunological disorders (Shin et al, 2016;Małaczewska et al, 2019;Qu et al, 2019). Based on this wide range of potential applications, many approaches have been attempted to improve the nisin yield, such as optimization of fermentation conditions, traditional random mutagenesis and selection, genome shuffling, and more (Wu et al, 2009;Tafreshi et al, 2010;Zhang et al, 2014). The growth of L. lactis, a major producer of nisin, is usually inhibited by the high concentration of lactic acid secreted during fermentation (Zhang et al, 2014), which has become the greatest challenge to the biotechnological engineering of this strain.…”

Over 2000-Fold Increased Production of the Leaderless Bacteriocin Garvicin KS by Increasing Gene Dose and Optimization of Culture Conditions

“…Nevertheless, poor production is often a bottleneck in large-scaled production of bacteriocins. Previous studies have shown that bacteriocin production can be increased by optimization of growth conditions such as cultivation temperature, pH, aeration and growth medium (Biswas et al, 1991; Parente and Ricciardi, 1994; Aasen et al, 2000; Cabo et al, 2001; Nel et al, 2001; Guerra and Pastrana, 2002; Penna and Moraes, 2002; Tafreshi et al, 2010). In addition, various heterologous expression systems have been reported for increased bacteriocin production (Horn et al, 2004; Kong and Lu, 2014; Jimenez et al, 2015; Jiang et al, 2016; Mesa-Pereira et al, 2017).…”