Optimization of Mixing Temperature and Sonication Duration in Liposome Preparation

Putri, Dina Christin Ayuning; Dwiastuti, Rini; Marchaban, Marchaban; Nugroho, Akhmad Kharis

doi:10.24071/jpsc.142728

Cited by 80 publications

(58 citation statements)

References 8 publications

(12 reference statements)

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“…PDI and PS of lipid‐based carriers are critical factors influencing the appearance, bulk properties, stability, processability, and performance of the encapsulated products (Danaei et al., ). Phospholipid vesicles with PDI less than 0.3 is said to be mono disperse or homogenous, which is the tolerable limit for lipid‐based carriers, particularly those used for drug delivery (Putri, Dwiastuti, Marchaban, & Nugroho, ). Therefore, PDI of all LE‐ECHE samples were relatively mono dispersed and highly stable.…”

Section: Resultsmentioning

confidence: 99%

Liposomal Encapsulated Ethanolic Coconut Husk Extract: Antioxidant and Antibacterial Properties

Olatunde

Benjakul

Vongkamjan

et al. 2019

Journal of Food Science

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Characteristics of liposomal encapsulated ethanolic coconut husk extract (LE-ECHE) prepared using two levels of lipid phase (LP) containing soybean phosphatidylcholine/cholesterol mixture of 4:1 mol ratio (60 and 80 µmol/mL) and two ECHE concentrations (1% and 2%) were investigated. Poly-dispersity index, zeta-potential, and particle size of LE-ECHE samples were 0.22% to 0.28%, −70.4 to −53.63 mV, and 232 to 697.65 nm, respectively. Encapsulation efficiency of all samples was 75.25% to 90.11%. LE-ECHE prepared with LP content of 60 µmol/mL and 1% ECHE (LP60-EC1) was milky, whereas UN-EC1 (un-encapsulated ECHE) was brownish in color. ECHE retained its antioxidant activity even after entrapment in liposome, although higher activity was recorded for UN-EC1. Encapsulation of ECHE in liposome enhanced antibacterial properties of ECHE. Hence, LP60-EC1 showed promising potential as a delivery based system for lowering dark color, a drawback associated with ECHE as well as improving the antibacterial properties of ECHE.Practical Application: Ethanolic coconut husk extract (ECHE) contains polyphenols with diverse biological activities such as antimicrobial and antioxidant properties. However, there are limited applications of ECHE in food industries, mainly because of its distinctive dark brown color. A homogeneous and stable liposomal system was demonstrated to be an efficient delivery based system for ECHE. Remarkably, antimicrobial property of ECHE was enhanced with liposomal encapsulation, whereas antioxidant activities of ECHE were retained. Also, liposomal encapsulation was shown as the potential technique to mask the undesirable dark brown, a drawback associated with ECHE for wider application.

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Section: Resultsmentioning

confidence: 99%

Liposomal Encapsulated Ethanolic Coconut Husk Extract: Antioxidant and Antibacterial Properties

Olatunde

Benjakul

Vongkamjan

et al. 2019

Journal of Food Science

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“…Values of 0.2 and below are most commonly deemed acceptable in practice for polymer-based nanoparticle materials [ 82 ]. In drug delivery applications using lipid-based carriers, such as liposome and nanoliposome formulations, a PDI of 0.3 and below is considered to be acceptable and indicates a homogenous population of phospholipid vesicles [ 83 , 84 , 85 ]. Although the last edition of the FDA’s “Guidance for Industry” concerning liposome drug products [ 86 ] emphasizes the importance of size and size distribution as “critical quality attributes (CQAs)”, as well as essential components of stability studies of these products, it does not mention the criteria for an acceptable PDI.…”

Section: Polydispersity Indexmentioning

confidence: 99%

Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems

et al. 2018

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Lipid-based drug delivery systems, or lipidic carriers, are being extensively employed to enhance the bioavailability of poorly-soluble drugs. They have the ability to incorporate both lipophilic and hydrophilic molecules and protecting them against degradation in vitro and in vivo. There is a number of physical attributes of lipid-based nanocarriers that determine their safety, stability, efficacy, as well as their in vitro and in vivo behaviour. These include average particle size/diameter and the polydispersity index (PDI), which is an indication of their quality with respect to the size distribution. The suitability of nanocarrier formulations for a particular route of drug administration depends on their average diameter, PDI and size stability, among other parameters. Controlling and validating these parameters are of key importance for the effective clinical applications of nanocarrier formulations. This review highlights the significance of size and PDI in the successful design, formulation and development of nanosystems for pharmaceutical, nutraceutical and other applications. Liposomes, nanoliposomes, vesicular phospholipid gels, solid lipid nanoparticles, transfersomes and tocosomes are presented as frequently-used lipidic drug carriers. The advantages and limitations of a range of available analytical techniques used to characterize lipidic nanocarrier formulations are also covered.

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“…The numerical value of PDI ranges from 0.0 for perfectly uniform sample with respect to particle size whereas a value approaching 1.0 represents a highly polydisperse sample with multiple particle size populations. In drug delivery applications using lipid based carriers such as liposomes and niosomes formulations, a PDI of 0.3 and below is considered to be acceptable and indicates a homogenous population of phospholipid vesicles [34][35][36] The optimized niosomal formulations showed polydispersity index in the range of 0.114 to 0.514 given in (table 3) indicating the homogeneity of the formulations.…”

Section: Vesicle Size Zeta Potential and Polydispersity Indexmentioning

confidence: 99%

In Vitro in Vivo Evaluation of Niosomal Formulation of Famotidine

Khan

Irchhaiya

2020

Int J Pharm Pharm Sci

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Objective: The present study was aimed on formulation and evaluation of famotidine loaded niosomal formulation for in vitro and in vivo pharmacokinetic behaviour. Formulating it as niosomal formulation might be quite advantageous for prolonging the duration of pharmacological action and improved bioavailability. Methods:In the present study niosomal formulations were prepared by using most documented thin film hydration technique by using various grades of surfactants (span 20, 40, 60, 80) in varying ratios with cholesterol, negative charge inducer di cetyl phosphate (DCP) and drug famotidine. Suitable preformulation studies were conducted like identification of drug, excipient and drug compatibility study. The optimized drug loaded niosomes were characterized for size and morphology, polydispersity index, zeta potential, drug entrapment, in vitro release, in vivo study and stability study. Results:The results showed that the vesicles formed were spherical in shape, size ranging between 160.1 nm to 718.7 nm with zeta potential values indicating good stability and formulation containing span 60 (NMS7) showed the highest entrapment efficiency (73.234%). All the formulations showed prolonged release profile for more than 24 h with release kinetics better suited to zero order release pattern. In vivo study conducted on rabbits predicted a fourfold increase in pharmacokinetic parameter (area under curve)AUC and pharmacological action for more than 24 h as compared to free drug famotidine which showed its action only upto 12 h. Conclusion:Thus the famotidine loaded niosomal formulation may be considered as a very promising drug delivery system which could be successfully employed for prolonging the drug release and overcoming the drawbacks of conventional drug delivery systems.

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Optimization of Mixing Temperature and Sonication Duration in Liposome Preparation

Abstract: ABSTRACT

Cited by 80 publications

References 8 publications

Liposomal Encapsulated Ethanolic Coconut Husk Extract: Antioxidant and Antibacterial Properties

Liposomal Encapsulated Ethanolic Coconut Husk Extract: Antioxidant and Antibacterial Properties

Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems

In Vitro in Vivo Evaluation of Niosomal Formulation of Famotidine

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