In Vitro in Vivo Evaluation of Niosomal Formulation of Famotidine

Abstract

Objective: The present study was aimed on formulation and evaluation of famotidine loaded niosomal formulation for in vitro and in vivo pharmacokinetic behaviour. Formulating it as niosomal formulation might be quite advantageous for prolonging the duration of pharmacological action and improved bioavailability. Methods:In the present study niosomal formulations were prepared by using most documented thin film hydration technique by using various grades of surfactants (span 20, 40, 60, 80) in varying ratios with cholesterol, negative charge inducer di cetyl phosphate (DCP) and drug famotidine. Suitable preformulation studies were conducted like identification of drug, excipient and drug compatibility study. The optimized drug loaded niosomes were characterized for size and morphology, polydispersity index, zeta potential, drug entrapment, in vitro release, in vivo study and stability study. Results:The results showed that the vesicles formed were spherical in shape, size ranging between 160.1 nm to 718.7 nm with zeta potential values indicating good stability and formulation containing span 60 (NMS7) showed the highest entrapment efficiency (73.234%). All the formulations showed prolonged release profile for more than 24 h with release kinetics better suited to zero order release pattern. In vivo study conducted on rabbits predicted a fourfold increase in pharmacokinetic parameter (area under curve)AUC and pharmacological action for more than 24 h as compared to free drug famotidine which showed its action only upto 12 h. Conclusion:Thus the famotidine loaded niosomal formulation may be considered as a very promising drug delivery system which could be successfully employed for prolonging the drug release and overcoming the drawbacks of conventional drug delivery systems.

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“…The independent variable (Factor B) was found to have a positive effect initially and reduces with further increase. The polydispersity index was observed negative with the independent variable (Factor C) and reflecting improved homogeneity in niosomal formulation [50].…”

Novasome: A Pioneering Advancementin Vesicular Drug Delivery

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Carmustine Loaded Nanosize Lipid Vesicles Showed Preferential Cytotoxicity and Internalization in U87mg Cell Line Along With Improved Pharmacokinetic Profile in Mice: A Strategy for Treatment of Glioma

“…For the determination of mean vesicle diameter (Z-average), polydispersity index (PDI) and overall surface charge (zeta potential) of the experimental formulations, a weighed amount of the formulation was dispersed in milli Q water, sonicated for 5 min and observed under a dynamic light scattering (DLS) instrument (DLS-nano ZS, Zetasizer, Malvern Instrument Ltd, UK) [16]. The data was interpretated by the instrument software.…”

“…After that, it was vortexed for another 3 min followed by centrifugation at 15 000 rpm. After centrifugation, the sediments were discarded and the absorbance of the collected supernatant was measured at 230 nm in UV-visible spectrophotometer (Advanced Microprocessor UV-Visible single beam, Intech 295, India) [15,16].…”

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