Optimization of medium for phenylalanine ammonia lyase production in E. coli using response surface methodology

Cui, Jiandong

doi:10.1007/s11814-009-0234-3

Cited by 20 publications

(11 citation statements)

References 17 publications

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“…Response surface methodology is a statistical method that can be used to study the optimum conditions under which the interactions between factors reach the maximum response value [30][31][32]. In this study, FFD experiments determined three key factors with important effects on response value.…”

Section: Central Composite Design and Response Surface Methodologymentioning

confidence: 99%

Enhanced adenosine triphosphate production by Saccharomyces cerevisiae using an efficient energy regeneration system

Yao

Xiong

Chen

et al. 2010

Korean J. Chem. Eng.

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The process of ATP biosynthesis from adenosine catalyzed by Saccharomyces cerevisiae was studied using an efficient energy regeneration system. A fractional factorial design (2 9-5 ) was used to evaluate the effects of different components in the medium. Magnesium chloride, toluene, and acetaldehyde were found to significantly influence ATP production. The concentrations of the three factors were then optimized using central composition design and response surface analysis. Based on the second-order polynomial model obtained from the experiments, the optimal parameters were obtained as follows: adenosine 20 g/L; glucose 67 g/L; S. cerevisiae cells 250 g/L; magnesium chloride 4.37 g/L; potassium dihydrogen phosphate 67 g/L; toluene 1.40 mL/L; acetaldehyde 2.67 mL/L; pH 7.0; and temperature 37.0 o C. Under the condition, the yield and concentration of ATP reached 97.5% and 37 g/L, respectively. The yield was nearly 10% higher than the level before optimization and the concentration increased two-fold. In addition, the utilization efficiency of energy after optimization increased nearly 6%.

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Section: Central Composite Design and Response Surface Methodologymentioning

confidence: 99%

Enhanced adenosine triphosphate production by Saccharomyces cerevisiae using an efficient energy regeneration system

Yao

Xiong

Chen

et al. 2010

Korean J. Chem. Eng.

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“…In this study, the five factors, viz. salinity (NaCl, m/V, %), Tween80 (V/V, %), temperature (˚C), pH, and dye concentration (mg/L), were chosen on the basis of previous studies [37,[39][40][41], and they were optimized to enhance the target dye Congo Red decolorization rate by the adsorbents obtained from T. pubescens pellets. Table 2 listed the levels and actual values of the five variables as well as the experimental design and results of BBD.…”

Section: Decolorization Rate-dependent Influencing Factors Optimizationmentioning

confidence: 99%

Dye Congo Red adsorptive decolorization by adsorbents obtained fromTrametes pubescenspellets

Si¹,

Cui²

2013

Desalination and Water Treatment

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A B S T R A C TA design of various dyes decolorization was conducted with the adsorbents obtained from Trametes pubescens pellets in submerged cultures. The results demonstrated that the adsorbents prepared by moist heat sterilization exhibited preferable decolorization capacity on azo dye Congo Red. A maximum decolorization rate of Congo Red of 72.31% was observed, which was all around 2.25 times higher than that of anthraquinone dye Disperse Blue B at the same level. Moreover, the decolorization rate-dependent influencing factors, i.e. salinity, Tween80, temperature, pH, and dye concentration, were optimized by Box-Behnken full factorial design. When they were 1.06% (m/V), 3.50% (V/V), 41.0˚C, 6.15, and 114.34 mg/L respectively, the decolorization rate was up to 100% after a 7-day incubation period. Adsorptive decolorization was caused by the electrostatic forces between dye molecules and adsorbents obtained from T. pubescens pellets, amido group played a major role during the biosorption process, and this course did not alter any functional groups at all, as evidenced by fourier transform infrared spectroscopy, chemical modifications, and scanning electron microscope data. All the results indicated that the adsorbents performed well at lowering the possible limitations arising out of the poor stability and enhancing convenient operability.

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“…An alternate strategy is a statistical approach such as orthogonal array method (OAM) and response surface method (RSM), involving the minimum number of experiments for a large number of factors [4,22].…”

Section: Most Commercial Cellulases Have Been Produced Bymentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

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Optimization of medium for phenylalanine ammonia lyase production in E. coli using response surface methodology

Cited by 20 publications

References 17 publications

Enhanced adenosine triphosphate production by Saccharomyces cerevisiae using an efficient energy regeneration system

Enhanced adenosine triphosphate production by Saccharomyces cerevisiae using an efficient energy regeneration system

Dye Congo Red adsorptive decolorization by adsorbents obtained fromTrametes pubescenspellets

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

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