Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by <i>Lentinus squarrosulus</i>

Abstract

The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for both mycelium biomass and EPS production. The yeast extract concentrations and initial pH showed the strongest effect (p < 0.001) meanwhile sucrose concentrations showed significant value at p < 0.005 for mycelium biomass. For EPS production, only sucrose and yeast extract concentrations gave significant value with p < 0.001 and p < 0.005 respectively. The model was validated by applying the optimized conditions and 25.8 ± 0.9 g/L of mycelium biomass, and 5.7 ± 0.4 mg/mL EPS concentration was obtained. The estimated optimum conditions of the variables for the production of mycelium biomass and EPS production by Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81; sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

Antioxidant Properties and Glucan Compositions of Various Crude Extract from &lt;i&gt;Lentinus squarrosulus&lt;/i&gt; Mycelial Culture

“…Submerged liquid fermentation was carried out for production of mycelium using optimized culture conditions (sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81) as described by [2]. A 5 mm plug of Lentinus sq.…”

“…The tough fruit body is rich in proteins, sugars, lipid, amino acids, vitamin B, C, and D, and minerals [1]. Liquid fermentation of Lentinus squarrosulus mushroom produces high amounts of uniform mycelia biomass in the culture broth which was found as source of polysaccharide [2]. Previous research reported that batch cultures using Pleurotus ferulae, Pleurotus nebrodensis, and Agrocybe aegerita effectively produced polysaccharides and mycelia growth from liquid cultures [3]- [7].…”

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by &lt;i&gt;Lentinus squarrosulus&lt;/i&gt;

“…To date there are only two reports on the effect of nutrient factors on vegetative mycelia growth in L. squarrosulus [15] [16]. The authors studied the effect of medium components on only one strain, but did not consider agitation and scalability.…”

Optimization of Biomass Production of Two Ectomycorrhizal Fungi (Lactarius quieticolor and Rhizopogon roseolus) for the Future Pinus radiata Nursery Inoculation

“…For the ectomycorrhizal fungus P. tinctorius (Srinivasan et al, 2000), an optimum growth range was found at pH 5.8-6 and a temperature of 29-30°C; when cultivated between 40 and 45 days in these conditions, the maximum biomass production was 1.13 g L -1 , far below what was reported for L. quieticolor and R. roseolus and mainly due to the slow growth of P. tinctorius in artificial culture media. Other biomass optimization processes have found a 1.4-fold increase in the biomass production of the saprophytic fungus C. versicolor (Wang et al, 2012) and 1.9-fold in the entomopathogenic fungus C. militaris (Ahmad et al, 2013). This underscores the importance of applying statistical designs to produce fungal biomass.…”

“…Examples of this include research being conducted on the entomopathogenic fungus Cordyceps militaris and Metarhizium rileyi for biomass production (Hsieh et al, 2009;Cui and Yuan, 2011;Song et al, 2017), saprophytic fungi Lentinus squarrosulus (Ahmad et al, 2013) for mycelium and exopolysaccharide production, Ganoderma lucidum for phenolic compound production and biomass (Zárate et al, 2013;Goh et al, 2016), Pleurotus sp. for enzyme production (Saravanakumar et al, 2010) and Filamentous Fungi for Extracellular Lipase Production (Gaurav et al, 2017).…”