Antioxidant Properties and Glucan Compositions of Various Crude Extract from <i>Lentinus squarrosulus</i> Mycelial Culture

Abstract

Various crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth were prepared for antioxidant studies, glucan and carbohydrate compound analysis. Crude extract from ethanol, cold and hot water extraction was tested on several complementary test systems namely DPPH free radical scavenging, β-carotene linoleic acid oxidation, total phenolic compound, total flavonoid compound, total carbohydrate content and total glucan compositions. The antioxidant activity of the crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth was determined by 2,2' diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and β-carotene linoleic acid assay. Crude extracted from culture broth EPS EE recorded the highest radical scavenging effects at 20 mg/mL (85%) which is closer to the control antioxidants (ascorbic acid 97%; BHT 92.4%; BHA 93.6%). Both the ethanol extract (IPS EE and EPS EE) exhibited the highest β-carotene linoleic acid oxidation by recording IC50 values of 0.6 and 0.65 mg/mL respectively. IPS EE (0.59 mg/g) and EPS WE (0.58 mg/g) showed high flavonoid content whereas total phenol content was high in EPS WE (0.85 mg/g) and IPS EE (0.84 mg/g). Total carbohydrate concentrations were high in IPS HWE and IPS CWE with 19.1 and 16.3 mg/mL respectively and these data correlate with the β-glucan content in both extracts. The highest β-glucan content found in IPS CWE and IPS HWE were 12.2 and 11.4% w/w respectively.

Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway

“…Firstly, chemical parameters of MLHWP were analysed spectroscopically which can be ranked in the following order: carbohydrates > protein (Table ). Carbohydrates, especially β‐glucan constituted the major proportion of this crude polysaccharide and its relative composition of is very much allied with previous published research findings on Russula alatoreticula , Macrocybe gigantea , Lentinus squarrosulus , Ganoderma applanatum and others.…”

Antioxidant Activities of Methanolic Extracts From Ten Pleurotus Species

“…P. ostreatus showed highest FRS activity in different organic and inorganic extract was ranged 58.76 % to 89.93 % at a concentration of 20 mg/ml 3 . Crude Extract from Lentinus squarrosulus mycelial culture showed highest FRS activity of 86 % at 20 mg/ml concentration 22 . Highest percentage of DPPH scavenging activity at 4 mg/ml concentration of Pleurotus pulmonarius showed 87.17±0.86; Pleurotus eryngii showed 75.00±1.88 and EC50 was 1.21, 2.67 mg/ml, respectively 23 but Ferreira et al 24 found EC50 value of Pleurotus eryngii was 9.21 mg/ml.…”

Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc

“…It has been reported that mushrooms contain different compounds of phenolics. Some authors have stated that mushrooms contain mainly phenolics acids [7,50], while others reported a high amount of flavonoids in extracts [5,37,51]. The total flavonoid content in different species of Pleurotus was found at the level from 1.2 to 2.9 µg/g of extract [49], even to 7.79 mg/g of extract [37].…”